Hijacking of the jasmonate pathway by the mycotoxin fumonisin B1 (FB1) to initiate programmed cell death in Arabidopsis is modulated by RGLG3 and RGLG4

Zhang, Xu; Wu, Qian; Cui, Shao; Ren, Jiao; Qian, Wanqiang; Yang, Yang; He, Shuchang; Chu, Jinfang; Sun, Xiaohong; Yan, Cunyu; Yu, Xiangchun; Chen, An

doi:10.1093/jxb/erv068

Cited by 29 publications

(18 citation statements)

References 73 publications

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“…Treated seedlings were collected for GUS staining, which was performed as described previously (Zhang et al, 2012), and stained plants were visualized under a Leica E24HD stereo microscope. GUS activity was measured as reported recently (Zhang et al, 2015). Briefly, total protein was extracted from 7-d-old seedling tissues using GUS extraction buffer (50 mM sodium phosphate buffer, pH 7.5, 10 mM b-mercaptoethanol, 0.1% Triton X-100, and 10 mM EDTA), followed by quantification by Bradford assays.…”

Section: Gus Assaysmentioning

confidence: 99%

“…RGLG2 catalyzes the formation of ubiquitin Lys-63linked chains when combined with the heterodimeric E2 enzyme formed by MMZ2 and UBC35 (Yin et al, 2007). Recently, it was reported that RGLG3 and RGLG4 are essential regulators of the jasmonate pathway (Zhang et al, 2012(Zhang et al, , 2015. However, in these studies, the molecular targets of RGLG1-4 were not identified.…”

Section: Introductionmentioning

confidence: 98%

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Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

et al. 2016

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Abscisic acid (ABA) is an essential hormone for plant development and stress responses. ABA signaling is suppressed by clade A PP2C phosphatases, which function as key repressors of this pathway through inhibiting ABA-activated SnRK2s (SNF1-related protein kinases). Upon ABA perception, the PYR/PYL/RCAR ABA receptors bind to PP2Cs with high affinity and biochemically inhibit their activity. While this mechanism has been extensively studied, how PP2Cs are regulated at the protein level is only starting to be explored. RING DOMAIN LIGASE5 (RGLG5) belongs to a five-member E3 ubiquitin ligase family whose target proteins remain unknown. We report that RGLG5, together with RGLG1, releases the PP2C blockade of ABA signaling by mediating PP2CA protein degradation. ABA promotes the interaction of PP2CA with both E3 ligases, which mediate ubiquitination of PP2CA and are required for ABA-dependent PP2CA turnover. Downregulation of and stabilizes endogenous PP2CA and diminishes ABA-mediated responses. Moreover, the reduced response to ABA in germination assays is suppressed in the (artificial microRNA) triple mutant, supporting a functional link among these loci. Overall, our data indicate that RGLG1 and RGLG5 are important modulators of ABA signaling, and they unveil a mechanism for activation of the ABA pathway by controlling PP2C half-life.

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Section: Gus Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

et al. 2016

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“…These E3 ligases have been reported to function redundantly as positive regulators of JA responses [ 99 ]. Furthermore, RGLG3 and RGLG4 are involved in the regulation of crosstalk between SA and JA in response to infection with the fungal pathogen Fusarium moniliforme [ 98 ]. The molecular mechanisms underlying RGLG3 and RGLG4 function in these processes and the ubiquitination target(s) of RGLG3 and RGLG4 remain to be identified.…”

Section: Ubiquitination In Ja Signaling Beyond the Core Modulementioning

confidence: 99%

The Ubiquitin System and Jasmonate Signaling

Durand

Pauwels

Goossens

2016

Plants

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The ubiquitin (Ub) system is involved in most, if not all, biological processes in eukaryotes. The major specificity determinants of this system are the E3 ligases, which bind and ubiquitinate specific sets of proteins and are thereby responsible for target recruitment to the proteasome or other cellular processing machineries. The Ub system contributes to the regulation of the production, perception and signal transduction of plant hormones. Jasmonic acid (JA) and its derivatives, known as jasmonates (JAs), act as signaling compounds regulating plant development and plant responses to various biotic and abiotic stress conditions. We provide here an overview of the current understanding of the Ub system involved in JA signaling.

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“…FB1 hijacks the JA pathway to initiate PCD. FB1 induces JA-responsive defense genes but represses growth-related and JA biosynthesis-related genes, thereby reducing JA contents in plants (Zhang et al, 2015). Furthermore, the ubiquitin ligases ring domain ligase 3 (RGLG3) and RGLG4 coordinately and positively regulate FB1triggered PCD by modulating the JA signaling pathway in a coronatine insensitive 1 (COI1)-and MYC2-dependent manner in Arabidopsis (Zhang et al, 2015).…”

Section: Hormone Signalingmentioning

confidence: 99%

“…Indeed, SA promotes FB1-induced cell death (Smith et al, 2015). Moreover, FB1-induced SA likely suppresses the JA pathway to facilitate cell death, since exogenously applied SA inhibited JA signaling additively with FB1 treatment (Zhang et al, 2015).…”

Section: Hormone Signalingmentioning

confidence: 99%

Fumonisin B1: A Tool for Exploring the Multiple Functions of Sphingolipids in Plants

Zeng

Yao

2020

Front. Plant Sci.

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Fumonisin toxins are produced by Fusarium fungal pathogens. Fumonisins are structural analogs of sphingosine and potent inhibitors of ceramide synthases (CerSs); they disrupt sphingolipid metabolism and cause disease in plants and animals. Over the past three decades, researchers have used fumonisin B1 (FB1), the most common fumonisin, as a probe to investigate sphingolipid metabolism in yeast and animals. Although the physiological effects of FB1 in plants have yet to be investigated in detail, forward and reverse genetic approaches have revealed many genes involved in these processes. In this review, we discuss the intricate network of signaling pathways affected by FB1, including changes in sphingolipid metabolism and the effects of these changes, with a focus on our current understanding of the multiple effects of FB1 on plant cell death and plant growth. We analyze the major findings that highlight the connections between sphingolipid metabolism and FB1-induced signaling, and we point out where additional research is needed to fill the gaps in our understanding of FB1-induced signaling pathways in plants.

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Hijacking of the jasmonate pathway by the mycotoxin fumonisin B1 (FB1) to initiate programmed cell death in Arabidopsis is modulated by RGLG3 and RGLG4

Abstract: HighlightTwo ubiquitin ligases control fumonisin B1-elicited programmed cell death by modulating jasmonate signalling transduction in Arabidopsis.

Cited by 29 publications

References 73 publications

Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

The Ubiquitin System and Jasmonate Signaling

Fumonisin B1: A Tool for Exploring the Multiple Functions of Sphingolipids in Plants

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