Highly viscous polysaccharide produced by an Enterobacter isolate on a hemicellulose hydrolysate

Abstract

A new anionic extracellular polysaccharide (AEPS) with high molecular mass (1700 kD) was produced by an isolate tentatively identified as Enterobacter cloacae isolated from an acid hydrolysate of wood. The sugar composition of this AEPS is glucose, galactose, glucuronic acid, and fucose (5:4:4:11). Under specified conditions this AEPS is theologically comparable to xanthan.

Novel polysaccharide produced by Azotobacter vinelandii isolated from plant rhizosphere

“…However, a similar composition ie. glucose, galactose, fucose and galactnronic acid in the molar ratio (5 : 4 : 4 : 11) has been reported for the EPS from a different organism, Enterobacter cloacae, a known pathogen, only when grown in the presence of a wood mineral acid hydrolysate (Meade et al 1993). …”

Production and rheological properties of a succinoglycan from Pseudomonas sp. 31260 grown on wood hydrolysates

“…Temperature ("C) 0.05% peptone plus 0.05% yeast extract slightly increased EPS yield over other additive combinations (Meade et al 1993). An inoculum of 1% v/v of a 24-h starter culture was chosen for EPS production conditions as a compromise between introduction of a sufficient microbial population and limitation of nutrient carryover.…”

“…must both be tolerant of inhibitory substances present (Perego et al 1990) and utilize xylose as a primary source of carbon to grow on the TVA hydrolysate. Unlike an Enterobacter cloacae isolate that produced EPS exclusively on the TVA hydrolysate (Meade et al 1993(Meade et al ,1994, Pseudomonas 3 1260 is capable of EPS production on a varietv of substrates. In the former case in which it was hypothesized that EPS was produced as a protective mechanism against the noxious furfurals present, obviously the Pseudomonas 31260 EPS is not produced expressly for this purpose since it is also synthesized on a variety of carbon sources (Williams and Wimpenny 1977;Fisher 199 1).…”

Comparative methods for isolation of Volcaniella eurihalina exopolysaccharide

“…There are many methods for recovery EPS, such as extraction with NaOH (Savidge and Calvin, 1985), EDTA (Mian et al, 1978), NaCl (Read and Costerton, 1987), heating (Allen et al, 1987) or filtration (Ceming et al, 1988); and still more, the precipitation can be carried out using different organic and inorganic compounds (Beech et al, 1991;Meade et al, 1994;Mody et al, 1989). There are many methods for recovery EPS, such as extraction with NaOH (Savidge and Calvin, 1985), EDTA (Mian et al, 1978), NaCl (Read and Costerton, 1987), heating (Allen et al, 1987) or filtration (Ceming et al, 1988); and still more, the precipitation can be carried out using different organic and inorganic compounds (Beech et al, 1991;Meade et al, 1994;Mody et al, 1989).…”