Highly tunable bioactive fiber-reinforced hydrogel for guided bone regeneration

Dubey, Nileshkumar; Ferreira, Jéssica A.; Daghrery, Arwa; Aytaç, Zeynep; Malda, Jos; Bhaduri, Sarit B.; Bottino, Marco C.

doi:10.1016/j.actbio.2020.06.011

Cited by 83 publications

(73 citation statements)

References 44 publications

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“…[ 19,20 ] MEW has been considered as a suitable and advantageous approach for engineering tissue scaffolds, as it enables the production of scaffold geometries with unprecedented precision and resolution, which can mimic the complex fibrous 3D morphology of the natural extracellular matrix (ECM) to support cell growth and proliferation. [ 21,22 ] Furthermore, MEW has been mostly reported due to its ease of use, cost‐effectiveness, precise geometric control, and compatibility with various natural and synthetic materials. Micro/nanofibers were directly written on a substrate using molten fluid columns under strong electrical fields.…”

Section: Introductionmentioning

confidence: 99%

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Chen

Jiang

Zhu

et al. 2021

Macromolecular Bioscience

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Tissue engineering scaffolds provide an encouraging alternative for nerve injuries due to their biological support for nerve cell growth, which can be used for neuronal repair. Nerve cells have been reported to be mostly cultured on 2D scaffolds that cannot mimic the native extracellular matrix. Herein, highly ordered 3D scaffolds are fabricated for nerve cell culture by melt electrospinning writing, the microstructures and geometries of the scaffolds could be well modulated. An effective strategy for scaffold surface modification to promote nerve cell growth is proposed. The effects of scaffolds with different surface modifications, viz., plasma treatment, single poly-D-lysine (PDL) coating after plasma treatment, single laminin (LM) coating after plasma treatment, double PDL and LM coatings after plasma treatment, on PC12 cell growth are evaluated. Experiments show the scaffold modified with double PDL and LM coatings after plasma treatment facilitated the growth of PC12 cells most effectively, indicating the synergistic effect of PDL and LM on the growth of nerve cells. This is the first systematic and quantitative study of the effects of different scaffold surface modifications on nerve cell growth. The above results provide a versatile culture platform for growing nerve cells, and for recovery from peripheral nerve injury.

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Section: Introductionmentioning

confidence: 99%

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Chen

Jiang

Zhu

et al. 2021

Macromolecular Bioscience

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Section: Soft Network Compositesmentioning

confidence: 99%

“…[37] Similar to the previous studies, the combination of a porous MEW mesh with a soft material allows flexibility regarding the mechanical properties and additionally, for further modification by doping the hydrogels with bioactive cues. Dubey et al [72] upgraded a GelMA hydrogel with amorphous magnesium phosphate to improve the osteogenic ability of the reinforced constructs for guided bone regeneration, shown in Figure 6B…”

Section: Soft Network Compositesmentioning

confidence: 99%

Polymers for Melt Electrowriting

Kade

Dalton

2020

Adv Healthcare Materials

135

155

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Melt electrowriting (MEW) is an emerging high‐resolution additive manufacturing technique based on the electrohydrodynamic processing of polymers. MEW is predominantly used to fabricate scaffolds for biomedical applications, where the microscale fiber positioning has substantial implications in its macroscopic mechanical properties. This review gives an update on the increasing number of polymers processed via MEW and different commercial sources of the gold standard poly(ε‐caprolactone) (PCL). A description of MEW‐processed polymers beyond PCL is introduced, including blends and coated fibers to provide specific advantages in biomedical applications. Furthermore, a perspective on printer designs and developments is highlighted, to keep expanding the variety of processable polymers for MEW.

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“…A tissue engineering and regeneration application of written PCL fibers was demonstrated in 2020, by Dubey et al who MEW manufactured PCL scaffolds to evaluate their ability to reinforce GelMA hydrogels and improve the composite material’s osteogenic ability for guided bone regeneration [ 13 ]. A 3DDiscovery system by regenHU was used to MEW extrude PCL fibers at optimized parameters of 90 °C melt temperature, 0.07 MPa feed pressure, 26 G spinneret, −7 kV applied voltage, 4 mm air gap, and 40 mm/s translational speed to produce fibers with a mean diameter of 3.16 µm.…”

Section: Fiber Writing Of Biomedical Polymersmentioning

confidence: 99%

Near-Field Electrospinning and Melt Electrowriting of Biomedical Polymers—Progress and Limitations

King

Bowlin

2021

Polymers

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Near-field electrospinning (NFES) and melt electrowriting (MEW) are the process of extruding a fiber due to the force exerted by an electric field and collecting the fiber before bending instabilities occur. When paired with precise relative motion between the polymer source and the collector, a fiber can be directly written as dictated by preprogrammed geometry. As a result, this precise fiber control results in another dimension of scaffold tailorability for biomedical applications. In this review, biomedically relevant polymers that to date have manufactured fibers by NFES/MEW are explored and the present limitations in direct fiber writing of standardization in published setup details, fiber write throughput, and increased ease in the creation of complex scaffold geometries are discussed.

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Highly tunable bioactive fiber-reinforced hydrogel for guided bone regeneration

Cited by 83 publications

References 44 publications

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Polymers for Melt Electrowriting

Near-Field Electrospinning and Melt Electrowriting of Biomedical Polymers—Progress and Limitations

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