Highly Stretchable Microelectrode Array for Free-form 3D Neuronal Tissue

Shim, Chaeyun; Jo, Yehhyun; Kyeong, Hyo; Kim, Mi Kyung; Kim, Hyojung; Kook, Geon; Kim, Ki-Up; Son, Gi Hoon; Lee, Hyunjoo Jenny

doi:10.1109/mems46641.2020.9056250

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…Combined with no observable changes in marker expression throughout development, this suggests device implantation does not interfere with typical developmental processes, including sarcomere assembly. A similar approach was recently reported, in which stretchable polyimide arrays were sandwiched around brain organoids, creating a stretched “pocket” capable of conforming to the organoids ( Shim et al, 2020 ; Figure 1C ). This approach is more limited to the outside surface of the organoid, but may provide more flexibility with timing and application, as it does not need to be integrated from the beginning of organoid development.…”

Section: Recent Advances In Electrophysiology—applicability To Brain mentioning

confidence: 74%

“…(B) Mesh nanoelectronics ( Li et al, 2019 ) are integrated throughout organoids in early stages of development, allowing for whole-organoid longitudinal recording. (C) Stretchable polyimide “pocket” arrays ( Shim et al, 2020 ) provide large-scale coverage of organoid surfaces, sacrificing inner region access for integration flexibility. (D) Nanoelectronics embedded into biomaterial scaffolds ( Tian et al, 2012 ) offer tunability and biocompatible support for organoid growth, as well as integrated 3D recording.…”

Section: Recent Advances In Electrophysiology—applicability To Brain mentioning

confidence: 99%

See 1 more Smart Citation

Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements

Passaro

Stice

2021

Front. Neurosci.

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Brain organoids, or cerebral organoids, have become widely used to study the human brain in vitro. As pluripotent stem cell-derived structures capable of self-organization and recapitulation of physiological cell types and architecture, brain organoids bridge the gap between relatively simple two-dimensional human cell cultures and non-human animal models. This allows for high complexity and physiological relevance in a controlled in vitro setting, opening the door for a variety of applications including development and disease modeling and high-throughput screening. While technologies such as single cell sequencing have led to significant advances in brain organoid characterization and understanding, improved functional analysis (especially electrophysiology) is needed to realize the full potential of brain organoids. In this review, we highlight key technologies for brain organoid development and characterization, then discuss current electrophysiological methods for brain organoid analysis. While electrophysiological approaches have improved rapidly for two-dimensional cultures, only in the past several years have advances been made to overcome limitations posed by the three-dimensionality of brain organoids. Here, we review major advances in electrophysiological technologies and analytical methods with a focus on advances with applicability for brain organoid analysis.

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Section: Recent Advances In Electrophysiology—applicability To Brain mentioning

confidence: 74%

Section: Recent Advances In Electrophysiology—applicability To Brain mentioning

confidence: 99%

Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements

Passaro

Stice

2021

Front. Neurosci.

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“…However, it is very difficult to directly process electrodes on non-planar surfaces. The current research mainly uses flexible materials to prepare 2D microelectrodes first, and then changes the arrangement direction of the two-dimensional MEA by external forces such as folding or stretching, so as to realize the preparation of 3D MEA [103,104]. By microfabrication techniques, 3D-structured electrodes are able to measure high-quality intracellular and extracellular potential changes within single neuron [105].…”

Section: Current Modeling and Application Of Neural System-on-a-chip ...mentioning

confidence: 99%

Microfabrication and lab-on-a-chip devices promote in vitro modeling of neural interfaces for neuroscience researches and preclinical applications

Liu,

Yao,

Fan

et al. 2023

Biofabrication

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Neural tissues react to injuries through the orchestration of cellular reprogramming, generating specialized cells and activating gene expression that helps with tissue remodeling and homeostasis. Simplified biomimetic models are encouraged to amplify the physiological and morphological changes during neural regeneration at cellular and molecular levels. Recent years have witnessed growing interest in lab-on-a-chip technologies for the fabrication of neural interfaces. Neural system-on-a-chip devices are promising in vitro microphysiological platforms that replicate the key structural and functional characteristics of neural tissues. Microfluidics and microelectrode arrays are two fundamental techniques that are leveraged to address the need for microfabricated neural devices. In this review, we explore the innovative fabrication, mechano-physiological parameters, spatiotemporal control of neural cell cultures and chip-based neurogenesis. Although the high variability in different constructs, and the restriction in experimental and analytical access limit the real-life applications of microphysiological models, neural system-on-a-chip devices have gained considerable translatability for modeling neuropathies, drug screening and personalized therapy.

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“…However, MEAs still have some problems including their limited contact with the organoids and the inconvenience arising from organoid culture protocols that require frequent media exchange. Shim et al (2020) recently fabricated a 3D dual-MEA to overcome these problems by sandwiching brain organoids between two stretchable polyimide layers, in which high stretchability was engineered with serpentine interconnects ( 143 ).…”

Section: Advanced Analysis Tools For Brain Organoidsmentioning

confidence: 99%

Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture

Jang

Kim

Koh

et al. 2022

IJSC

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The emergence of brain organoids as a model system has been a tremendously exciting development in the field of neuroscience. Brain organoids are a gateway to exploring the intricacies of human-specific neurogenesis that have so far eluded the neuroscience community. Regardless, current culture methods have a long way to go in terms of accuracy and reproducibility. To perfectly mimic the human brain, we need to recapitulate the complex in vivo context of the human fetal brain and achieve mature neural circuitry with an intact cytoarchitecture. In this review, we explore the major challenges facing the current brain organoid systems, potential technical breakthroughs to advance brain organoid techniques up to levels similar to an in vivo human developing brain, and the future prospects of this technology.

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Highly Stretchable Microelectrode Array for Free-form 3D Neuronal Tissue

Cited by 4 publications

References 12 publications

Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements

Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements

Microfabrication and lab-on-a-chip devices promote in vitro modeling of neural interfaces for neuroscience researches and preclinical applications

Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture

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