Highly Stable PEGylated Poly(lactic-co-glycolic acid) (PLGA) Nanoparticles for the Effective Delivery of Docetaxel in Prostate Cancers

Abstract

In the present study, a highly stable luteinizing-hormone-releasing hormone (LHRH)-conjugated PEGylated poly(lactic-co-glycolic acid) (PLGA) nanoparticles were developed for the successful treatment of prostate cancers. We have demonstrated that a unique combination of targeted drug delivery and controlled drug release is effective against prostate cancer therapy. The docetaxel (DTX)/PLGA-LHRH micelles possessed a uniform spherical shape with an average diameter of ~170 nm. The micelles exhibited a controlled drug release for up to 96 h which can minimize the non-specific systemic spread of toxic drugs during circulation while maximizing the efficiency of tumor-targeted drug delivery. The LHRH-conjugated micelles showed enhanced cellular uptake and exhibited significantly higher cytotoxicity against LNCaP cancer cells. We have showed that PLGA-LHRH induced greater caspase-3 activity indicating its superior apoptosis potential. Consistently, LHRH-conjugated micelles induced threefold and twofold higher G2/M phase arrest than compared to free DTX or PLGA NP-treated groups. Overall, results indicate that use of LHRH-conjugated nanocarriers may potentially be an effective nanocarrier to effectively treat prostate cancer.

In Vitro Antifungal and Antivirulence Activities of Biologically Synthesized Ethanolic Extract of Propolis-Loaded PLGA Nanoparticles against Candida albicans

“…The amount of EEP in supernatant of EEP-NPs was calculated with the EEP standard calibration curve obtained by using the peak area. The EE and LC of EEP-NPs were quantified using the following equations [22]:EE%=amount of EEP encapsulated in NPsinitial EEP added×100,LC%=amount of EEP in NPstotal amount of NPs×100.…”

Synergistic Enhancement of Antitumor Efficacy by PEGylated Multi-walled Carbon Nanotubes Modified with Cell-Penetrating Peptide TAT

“…During the last few decades, increasing attention has been paid to multifunctional drug delivery systems using nanotechnology to improve the overall therapeutic efficacy of anticancer activity [ 1 , 2 ]. Among all those systems, carbon nanotubes (CNTs) are one of the most interesting nanocarries currently under investigation.…”

Sustained Inhibition of the IL4/IL13 Receptor Complex during Allergen Specific Immunotherapy

“…As In order to prolong the presence of IL-4 Mutein, which is characterized by a very short serum half-life, a PLGA-PEG-PLGA based hydrogel was tested as delivery matrix for its sustained release in vivo. The biodegradable (Choi et al, 2004), nonimmunogenic (Cao et al, 2016) Furthermore, the polymer was tested as delivery matrix of allergens during AIT.…”