Highly Stable Gold NanoparticlesusingGel as Reducing Agent and Stability: evaluation of its free radical scavenging activity

Abstract

The research was focused on the production, characterization and application of Gold nanoparticles (AuNP s), which can be utilized in biomedical research and environmental cleaning applications.In vitro free radical scavenging activity of synthesized gold nanoparticles (AuNPs) was investigated. There are many studies use chemical method to synthesis gold nanoparticles (AuNPs) using various chemicals do as reducing agents at different conditions. In this study, gold nanoparticles (AuNPs)were synthesized by gel as reducing and stabilizing agent to reduce Au +3 ions form chloroauric acid (HAuCl 4 .3H 2 O) to nano gold .The reactionwere required heating around 60-80 o C.Synthesis of colloidal Au NPs was monitored by UV-Visible spectroscopy. The UV-Visible spectrum showed 524 nm , The characterization of the AuNPs such as their size , shape and stabile was performed by zeta potential , Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM) techniques which indicated a size range of 14 to 25 nm. The in vitroantioxidant properties of AuNPs using DPPH assay have been evaluated and these nanoparticles were found to have