Highly stable enzyme precipitate coatings and their electrochemical applications

Immobilization and Stabilization of Acylase on Carboxylated Polyaniline Nanofibers for Highly Effective Antifouling Application via Quorum Quenching

“…The step of enzyme precipitation by adding ammonium sulfate is critical for the efficient formation of chemical linkages during the follow-up enzyme crosslinking by shortening the distance among enzymes when enzyme molecules are precipitated. [32][33] To investigate the contribution of enzyme leaching to the enzyme inactivation, the amount of 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 30% of their initial activities, respectively ( Figure S3...…”

Enzyme precipitate coatings of lipase on polymer nanofibers

“…As a bypass, the approach of EPC introduces the enzyme precipitation just before the enzyme crosslinking. By that way, the enzyme precipitates would allow for closer contact between LP molecules that potentially enables more effective enzyme crosslinking, as demonstrated in the case of EPC-GOx [22].…”

“…Interestingly, however, the EC approach could not stabilize the activity of glucose oxidase (GOx), and an approach of enzyme precipitate coating (EPC) was proposed as a bypass and employed for the successful stabilization of GOx on polymer nanofibers [22]. The EPC approach, consisting of covalent attachment, enzyme precipitation and enzyme crosslinking, enables effective enzyme crosslinking by allowing for close contact between GOx molecules in their precipitate form.…”

“…Especially, the recent developments of nanobiocatalysis have gathered growing attention due to their successful results in achieving high enzyme loading and stability [9]. Various nanostructured materials, such as mesoporous materials [10][11][12][13][14][15], nanoparticles [16][17][18], carbon nanotubes [19][20][21][22][23], and electrospun nanofibers [22,[24][25][26][27][28][29][30][31][32][33], have been used for enzyme immobilization because their large surface areas can improve the loading and activity of enzymes per unit mass of materials. Among them, polymer nanofibers offer many advantageous features, such as durability, easy recovery, and lower mass transfer limitation by immobilizing enzymes on the nanofiber surface [34,35].…”

Recent progress in nanobiocatalysis for enzyme immobilization and its application

“…In the viewpoint of stability, nanobiocatalysis can also provide an environment to prevent the enzyme from leaching and/or losing its 3-dimensional structure via nanoconfinement and/or multipoint attachment. Kim et al described the precipitate coating of glucose oxidase on a carbon nanotube as the anode in biofuel cell exhibited a 10% decrease of power density after 2 h incubation at 50 o C. However, the power density of the covalently attached glucose oxidase electrode was reduced by 92% after the same thermal treatment [49]. Kwon et al prepared a nanoscale enzyme reactor (NER) of glucose oxidase in conductive mesoporous carbon via a two-step process of adsorption and followed-up crosslinking for preventing enzyme leaching via nanoconfinement.…”