Highly specific Electrochemical Sensing of Pseudomonas aeruginosa in patients suffering from corneal ulcers: A comparative study

Abstract

Pseudomonas aeruginosa is the most common pathogenic gram-negative bacteria causing corneal ulcers globally. In severe cases, often after trauma and eye injury, corneal destruction progresses rapidly and may be completed within 24–48 h causing blindness. In our preliminary work, we have established an ultrasensitive polyaniline (PANI)/gold nanoparticles (Au NPs)/indium tin oxide (ITO) modified sensor for rapid detection of pyocyanin (PYO) in P. aeruginosa infections with a linear range from 238 μM to 1.9 μM and a detection limit of 500 nM. In the present study, we evaluated the efficiency of the established modified electrochemical sensor in the diagnosis of P. aeruginosa in 50 samples collected from patients suffering from corneal ulcers. The obtained results were compared with the results gained by the screen-printed electrode, conventional techniques, automated identification method, and the amplification of the 16 s rRNA gene by PCR as a gold standard test for P. aeruginosa identification. We have found that the electrochemical detection of PYO by square wave voltammetry technique using PANI/Au NPs modified ITO electrode was the only technique showing 100% agreement with the molecular method in sensitivity, specificity, positive and negative predictive values when compared with the SPE, conventional and automated methods.

Plasma‐Functionalized Isotactic Polypropylene Assembled with Conducting Polymers for Bacterial Quantification by NADH Sensing

“…In recent times, within the focus of biomedical applications, some relevant contributions to the utilization of sensors for the detection of bacterial growth using nonimpedance techniques have been published. [ 8–14 ] Cernat et al. [ 8 ] reported a nanohybrid sensor applicable to human serum, saliva, and tap water made of graphene, polypyrrole, and gold using pyoverdine as chemical marker, the siderophore of Pseudonomas aeruginosa ( P. aeruginosa ).…”

“…More recently, Khalifa et al. [ 9 ] utilized a polyaniline/gold nanoparticles‐modified indium tin oxide (ITO) electrode to detect pyocyanin in P. aeruginosa infections, using samples collected from patients suffering from corneal ulcers. Simoska et al.…”

Application of Conducting Polymer Nanostructures to Electrochemical Biosensors

“…The enhancement of the electrochemical activity of PANI/Au NPs/ITO modified electrode towards the PYO related to the presence of positive charges on its surface that could enhance the mass transfer rate of the negatively charged PYO based on the electrostatic attraction force. The same group has extended the uses of PANI/Au NPs/ ITO electrode for diagnosis of P. aeruginosa in 50 samples collected from patients suffering from corneal ulcers as shown in Figure 3b [43]. The obtained results were compared with the results gained by the screen-printed electrode (SPE), conventional techniques, automated identification method, and the amplification of the 16 s rRNA gene by polymerase chain reaction (PCR) as a standard test for P. aeruginosa identification.…”

“…Copyright 2014 Elsevier. (b) The schematic diagram for the fabrication of polyaniline (PANI)/Au NPs/ITO electrode and the interaction between PANI and pyocyanin (Pyocyanin (PYO)[43]. (c) Scheme depicting surface imprinted polymers (SIPs)-graphene oxide composites preparation on a gold surface for making the ZIKV biosensor.…”

Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein

“…Furthermore, electrochemical biosensors detect the most easy-to-use, accurate, quick, and sensitive biomarkers [14] , [20] , [21] . Several nanostructures modified electrodes have been reported to enhance the sensitivity and selectivity of the electrochemical biosensors [22] , [23] , [24] , [25] , [26] , [27] , [28] .…”