Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Batta, Ashok K.; Salen, Gerald; Rapole, Keshav R.; Batta, Manju; Batta, Priti; Alberts, David S.; Earnest, David L.

doi:10.1016/s0022-2275(20)33519-7

Cited by 78 publications

(9 citation statements)

References 19 publications

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“…We found that metabolites associated with primary and secondary bile acid metabolism subpathways were positively associated with CAC in AAs. Excess cholesterol is converted into bile acids in the liver and excreted from the body [ 30 ]. Compared to individuals without coronary artery disease (CAD), patients with CAD reportedly have lower fecal bile acid excretion, and this supports a potential protective effect of bile acid excretion on the development of CAD [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Plasma metabolomic profiling in subclinical atherosclerosis: the Diabetes Heart Study

Chevli

Freedman

Hsu

et al. 2021

Cardiovasc Diabetol

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Background Incidence rates of cardiovascular disease (CVD) are increasing, partly driven by the diabetes epidemic. Novel prediction tools and modifiable treatment targets are needed to enhance risk assessment and management. Plasma metabolite associations with subclinical atherosclerosis were investigated in the Diabetes Heart Study (DHS), a cohort enriched for type 2 diabetes (T2D). Methods The analysis included 700 DHS participants, 438 African Americans (AAs), and 262 European Americans (EAs), in whom coronary artery calcium (CAC) was assessed using ECG-gated computed tomography. Plasma metabolomics using liquid chromatography-mass spectrometry identified 853 known metabolites. An ancestry-specific marginal model incorporating generalized estimating equations examined associations between metabolites and CAC (log-transformed (CAC + 1) as outcome measure). Models were adjusted for age, sex, BMI, diabetes duration, date of plasma collection, time between plasma collection and CT exam, low-density lipoprotein cholesterol (LDL-C), and statin use. Results At an FDR-corrected p-value < 0.05, 33 metabolites were associated with CAC in AAs and 36 in EAs. The androgenic steroids, fatty acid, phosphatidylcholine, and bile acid metabolism subpathways were associated with CAC in AAs, whereas fatty acid, lysoplasmalogen, and branched-chain amino acid (BCAA) subpathways were associated with CAC in EAs. Conclusions Strikingly different metabolic signatures were associated with subclinical coronary atherosclerosis in AA and EA DHS participants.

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Section: Discussionmentioning

confidence: 99%

Plasma metabolomic profiling in subclinical atherosclerosis: the Diabetes Heart Study

Chevli

Freedman

Hsu

et al. 2021

Cardiovasc Diabetol

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“…It is known that cholesterol is mainly eliminated from the body via the liver in the form of bile acids [Batta et al 1999]. It is reasonable to speculate that a reduced ability to convert cholesterol to bile acids would lead to body cholesterol overload, with the subsequent development of atherosclerosis [Assmann et al 2006;Sudhop et al 2002;Rajaratnam et al 2000Rajaratnam et al , 1999Glucc et al1991].…”

Section: Introductionmentioning

confidence: 99%

The association of bile acid excretion and atherosclerotic coronary artery disease

Charach

Grosskopf

Rabinovich

et al. 2010

Therap Adv Gastroenterol

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Abstract:Background: Excess cholesterol is usually eliminated from the body by conversion to bile acids excreted in feces as bile salts. The excretion of large amounts of bile protects against atherosclerosis, while diminished excretion may lead to coronary artery disease (CAD). Objective: To investigate a relationship between CAD and bile acid excretion. Methods: Bile acid excretion was compared between 36 patients with proven CAD and 37 CAD-free individuals (controls). The groups were comparable for demographics and selected risk factors. All subjects received a 4-day standard diet that included $500 mg of cholesterol. Fecal bile acids from 24-hour stool collections were measured by gas liquid chromatography. Results: CAD patients excreted lower amounts of total bile acids (358±156 mg) than controls (617±293 mg; p < 0.01) and less deoxycholic acid (188.29±98.12 mg versus 325.96±198.57 mg; p < 0.0001) and less lithocholic acid (115.43±71.89 mg versus 197.27±126.87 mg; p < 0.01). Advanced age, male gender, left ventricular ejection fraction and total bile acid levels were significant independent factors that predicted CAD (p < 0.05). Mortality, CAD and cerebrovascular accident development rates were significantly lower for the controls at the 13-year follow up. Conclusion: CAD patients have significantly decreased bile acid excretion levels than non-CAD patients. An impaired ability to excrete cholesterol may be an additional risk factor for CAD development.

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“…Bile acids are biological detergents and metabolic regulators required for proper lipid digestion and absorption. In addition, bile acids facilitate the excretion of cholesterol and other molecules, such as xenobiotics ( Batta et al, 1999 ). Multiple studies have reported a negative correlation between CHD and bile acid excretion, which is also confirmed in our study ( Assmann et al, 2006 ; Gylling et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments

et al. 2021

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To improve the treatment of patients with coronary heart disease (CHD), personalized treatments based on potential biomarkers could make a difference. To investigate if such potential biomarkers could be found for CHD inhomogeneous, we combined traditional Chinese medicine based diagnosis with untargeted and targeted metabolomics analyses. Shi and Xu patient subtype groups of CHD with angina pectoris were identified. Different metabolites including lipids, fatty acids and amino acids were further analyzed with targeted metabolomics and mapped to disease-related pathways. The long-chain unsaturated lipids ceramides metabolism, bile acid metabolism were differentially affected in the Xu subtype groups. While, Shi-subtype patients seemed to show inflammation, anomalous levels of bioactive phospholipids and antioxidant molecules. Furthermore, variations in the endothelial damage response and energy metabolism found based on ELISA analysis are the key divergence points between different CHD subtypes. The results showed Xu subtype patients might benefit from long-chain unsaturated lipids ceramides as therapeutic targets. Shi subtype patients might benefit more from levels of polyunsaturated fatty acid consumption and treatments that help in restoring energy balance. Metabolic differences can be essential for treatment protocols. Thus, patient group specific differences can serve as important information to refine current treatment approaches in a personalized manner.

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Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Cited by 78 publications

References 19 publications

Plasma metabolomic profiling in subclinical atherosclerosis: the Diabetes Heart Study

Plasma metabolomic profiling in subclinical atherosclerosis: the Diabetes Heart Study

The association of bile acid excretion and atherosclerotic coronary artery disease

Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments

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