Highly sensitive spectrofluorimetric method for the determination of two antimigraine drugs in their tablets and in biological fluids. Application to content uniformity testing

Abstract

A highly sensitive and simple spectrofluorimetric method has been developed for the determination of almotriptan malate (ALM) and zolmitriptan (ZOL) in their pharmaceutical preparations. The proposed method is based on the investigation of the fluorescence spectral behavior of the two drugs in aqueous acidic systems. The fluorescence intensity was measured at 360 and 350 nm after excitation at 270 nm for ALM and ZOL, respectively. The fluorescence-concentration plots were rectilinear over the range 0.1-1.2 mg mL À1 and 5-40 ng mL À1 , with lower detection limits of 0.03 mg mL À1 and 0.70 ng mL À1 for ALM and ZOL, respectively. The proposed method was successfully applied for the assay of commercial tablets as well as for content uniformity testing. The application of the proposed method was extended to the assay of ZOL in biological fluids. The results were statistically compared to those obtained by comparison methods and were found to be in good agreement.

Validation of Rapid and Sensitive Spectrofluorimetric Assay for Determination of Four Triptans in Pure and Dosage Forms; Application to Human Plasma and Content Uniformity Testing

“…Application to assay of commercial samples: The proposed analytical procedure has been used for the analysis of pharmaceutical dosage forms of the investigated drugs and results obtained were compared by those of the described ones [3,5,8,11] by applying student's t-test and F-test at the 95% confidence level [38]. No significant difference was noticed between the values of the suggested and reported assays indicating high accuracy as well as good precision in the assay of the investigated compounds in their dosage forms (Table 5).…”

“…[1,2]. Different analytical techniques were reported for their determination in different matrices including spectrophotometry [3][4][5][6][7][8], spectrofluorimetry [9][10][11], conductometry [12] and HPLC [13][14][15][16][17][18][19][20]. The complex formation that occurs between a lot of drugs and Eosin Y as an ion pairing agent has been considerably investigated using spectrophotometric and spectrofluorimetric assay of such drugs with or without using of metal [21][22][23][24][25][26][27].…”

Two spectroscopic methods for estimation of anti‐migraine medications: Sumatriptan and Zolmitriptan based on nucleophilic substitution reaction

“…Method I Method II Comparison methods [4,26] Amount taken (μg/ml) Amount found (μg/ml) % found Amount taken (μg/ml) *The figures between parentheses are the tabulated t-and F-values at P = 0.05. [28] NB: Each result is the average of three separate determinations.…”

“…NBD-Cl is an electroactive halide reagent, which was considered as a likely target for good nucleophiles, the studied drugs have a secondary amino group in an indole ring considered as good nucleophile, and react with NBD-Cl through nucleophilic substitution forming a highly T A B L E 4 Assay results for determination of the SUM and ZOL in their tablet form by proposed method Preparation Amount taken (μg/ml) Amount found (μg/ml) % found Comparison method [4,26] *The figures between parentheses are the tabulated t-and F-values at P = 0.05. [28] NB: Each result was the average of three separate determinations.…”

“…The reported methods for its estimation include spectrophotometry, [8,[19][20][21] HPLC, [15,[22][23][24] liquid chromatographymass spectrometry (LC-MS) [25] and spectrofluorimetric methods. [9,26] The recent study helps to validate an uncomplicated, selective and sensitive spectrophotometric method and a spectrofluorimetric method for estimation of both drugs. The suggested methods were fully validated according to ICH guidelines.…”