Highly sensitive spectrofluorimetric determination of lomefloxacin in spiked human plasma, urine and pharmaceutical preparations

Ulu, Sevgi Tatar

doi:10.1016/j.ejmech.2009.02.019

Cited by 16 publications

(4 citation statements)

References 19 publications

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“…The detection limit and the limit of quantification were found to be 16 nM and 55 nM, calculated by using the relation 3σ/b and 10σ/b, respectively, where σ is the standard deviation of the blank solution and b is the slope of the calibration plot. Table II summarizes the comparison of analytical performances of voltammetric methods reported for the determination of LMF [30][31][32][33] and it can be seen that the developed sensor has superior detection limit over the ones reported earlier. The results show that the developed method has a potential for the sensitive determination of LMF in biological fluids.…”

Section: Resultsmentioning

confidence: 99%

A Sensitive Polymelamine Modified Sensor for the Determination of Lomefloxacin in Biological Fluids

Gupta

Yadav

Goyal

2014

J. Electrochem. Soc.

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A sensitive electrochemical method for lomefloxacin (LMF) determination has been developed by using poly-melamine layer modified glassy carbon electrode [p-(melamine)/GCE]. The surface morphology of the modified sensor was characterized by Horizontal Attenuated Total Reflectance-infrared spectroscopy (HATR-IR), Field emission scanning electron microscopy (FE-SEM) and electrochemical impedance spectroscopy (EIS). The electrochemical measurements were carried out using square wave voltammetry and cyclic voltammetry. The polymer modified electrode showed an excellent electrocatalytic activity toward the electrochemical oxidation of LMF with a well-defined voltammetric peak at ∼980 mV. The peak current of LMF was found to be linear in the range of 0.1–500 μM and a detection limit and sensitivity of 16 nM and 0.0346 μA μM−1, respectively, were observed. The modified electrode shows good selectivity toward the specific recognition of LMF in the presence of common biological interferents. The analytical applicability of the proposed method was successfully demonstrated in different pharmaceutical formulations, human urine and serum samples. The results obtained from the voltammetry were also validated with HPLC.

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Section: Resultsmentioning

confidence: 99%

A Sensitive Polymelamine Modified Sensor for the Determination of Lomefloxacin in Biological Fluids

Gupta

Yadav

Goyal

2014

J. Electrochem. Soc.

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“…[1,2] Several reports have described the determination of LMX in pure form, dosage forms and bio-fluids, using spectroscopic, [3][4][5][6][7][8][9][10][11][12][13][14][15] chromatographic [16][17][18][19][20][21][22][23] and electrochemical [24,25] methods. Despite some papers having been published for fluorimetric analysis of LMX, [10][11][12][13][14][15] the presented method has merits of being the most sensitive fluorimetric method for determination of LMX to date. A comparison of the developed method with other published fluorimetric methods for detection of LMX is shown in Supporting Information, Table S1.…”

Section: Introductionmentioning

confidence: 99%

A novel spectrofluorimetric method for determination of lomefloxacin adopting on zinc(II) chelation strategy: Application in human plasma

et al. 2021

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A new sensitive and instantaneous spectrofluorimetric method for efficient determination of lomefloxacin (LMX) in its pure, dosage form and human plasma was designed.The developed method depends on formation of a metal-chelation compound of LMX as a ligand with zinc(II) in a buffer of acetate (pH 5.5). The following parameters; type of metal, concentration of metal, pH, type of buffer and diluting solvent were optimized. After carefully investigation; 0.2 mM zinc, 2.0 ml acetate buffer (pH 5.5) and water as diluting solvent were set as optimum reaction conditions. Under these conditions, a large increase in the intensity of the fluorescence of LMX was attained at 450 after excitation at 284 nm. The limits of detection and quantification were 5.8 and 1.9 ng ml À1 , respectively, with linearity range of 10.0 to 500.0 ng ml À1 . The binding mode of LMX and zinc(II) ion (Zn 2+ ) was found to be 2:1, respectively, and confirmed by Job's plot method. Furthermore, it extended to the analysis of LMX in the spiked plasma of humans with percentage recovery (98.70 ± 0.97 to 100.30 ± 1.69%, n = 3).

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“…Fluorescence spectrometry is an exceptionally quick, efficient, selective, and profoundly responsive procedure for evaluation of drug in plasma. [13][14][15][16] Extensive literature review revealed that report describing an analytical…”

Section: Introductionmentioning

confidence: 99%

<p>Validated Microwell-Based Spectrofluorimetric Method for Quantification of Ravidasvir (New Anti-Chronic Hepatitis C Virus-GT4) in Rat Plasma and Its Application to Pharmacokinetic Study</p>

Hefnawy

Al‐Shehri

Al-Rashood

et al. 2020

DDDT

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Background: Ravidasvir (RAV) has been regarded as a potent new NS5A inhibitor with a magnificent safety and tolerability in the management of genotype 4 hepatitis C virus (HCV) patients. Suitable analytical techniques are needed for the measurement of RAV in different biological matrices. Methods: We have developed a fast, sensitive and economical 96-microwell-based spectrofluorimetric technique combined with one-step protein precipitation extraction strategy for the measurement of RAV in rat plasma. Results: Under the optimum conditions, the direct relationship in rat plasma was accomplished between the RAV concentrations and the fluorescence (FL) intensity in a scope of 2.5-200 ng/mL with 0.9998 and 0.9999 for the quantification and correlation coefficients, respectively. The lower limit of detection (LLOD) was 0.840 ng/mL and this demonstrates the high sensitivity of the proposed assay. The accuracy (RE%) ranged from 95.34% to 102.29%, and the precision (RSD%) was less than 3.59%. The recovery was ranged from 93.12% to 96.26%. The stability of RAV in rat plasma was carried out and established its good stability in the range of room conventional temperature and at long-term stability (−80° C, 30 days). The developed technique was validated as stated by the United States Food and Drug Administration (US-FDA) guidelines for bioanalytical technique verification. Conclusion: The approved technique was effectively applied for a pharmacokinetic (PK) study after single oral gavage administration of RAV at a dose of 35 mg/kg and it could be presumed that the proposed assay can be applied to clinical trials.

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Highly sensitive spectrofluorimetric determination of lomefloxacin in spiked human plasma, urine and pharmaceutical preparations

Cited by 16 publications

References 19 publications

A Sensitive Polymelamine Modified Sensor for the Determination of Lomefloxacin in Biological Fluids

A Sensitive Polymelamine Modified Sensor for the Determination of Lomefloxacin in Biological Fluids

A novel spectrofluorimetric method for determination of lomefloxacin adopting on zinc(II) chelation strategy: Application in human plasma

<p>Validated Microwell-Based Spectrofluorimetric Method for Quantification of Ravidasvir (New Anti-Chronic Hepatitis C Virus-GT4) in Rat Plasma and Its Application to Pharmacokinetic Study</p>

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