Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

Rakovich, Tatsiana; Mahfoud, Omar K.; Mohamed, Bashir M.; Prina-Mello, Adriele; Crosbie-Staunton, Kieran; Broeck, Tina Van Den; Kimpe, Line De; Sukhanova, Alyona; Baty, Daniel; Rakovich, Aliaksandra; Maier, Stefan A.; Alves, Frauke; Nauwelaers, Frans; Nabiev, Igor; Chames, Patrick; Volkov, Yuri

doi:10.1021/nn500212h

Cited by 90 publications

(44 citation statements)

References 62 publications

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“…In most cases, antibodies appended with fluorescent molecules or nanomaterials are used to visualize these endogenous biomarkers on living cell surfaces. [116][117][118][119] However, the intrinsic fluorescent property of these components inevitably requires additional washing operations for accurate molecular imaging on cells. The target-responsive fluorescence switching mechanism is particularly promising to solve this problem.…”

Section: Cancer Protein Biomarkersmentioning

confidence: 99%

Cancer biomarker detection: recent achievements and challenges

2015

Chem. Soc. Rev.

948

568

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The early detection of cancer can significantly reduce cancer mortality and saves lives. Thus, a great deal of effort has been devoted to the exploration of new technologies to detect early signs of the disease. Cancer biomarkers cover a broad range of biochemical entities, such as nucleic acids, proteins, sugars, small metabolites, and cytogenetic and cytokinetic parameters, as well as entire tumour cells found in the body fluid. They can be used for risk assessment, diagnosis, prognosis, and for the prediction of treatment efficacy and toxicity and recurrence. In this review, we provide an overview of recent advances in cancer biomarker detection. Several representative examples using different approaches for each biomarker have been reviewed, and all these cases demonstrate that the multidisciplinary technology-based cancer diagnostics are becoming an increasingly relevant alternative to traditional techniques. In addition, we also discuss the unsolved problems and future challenges in the evaluation of cancer biomarkers. Clearly, solving these hurdles requires great effort and collaboration from different communities of chemists, physicists, biologists, clinicians, material-scientists, and engineering and technical researchers. A successful outcome will result in the realization of point-of-care diagnosis and individualized treatment of cancers by non-invasive and convenient tests in the future.

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Section: Cancer Protein Biomarkersmentioning

confidence: 99%

Cancer biomarker detection: recent achievements and challenges

2015

Chem. Soc. Rev.

948

568

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“…This shows that the use of QD-based labels in fluorogenic assays is decisively advantageous. Additionally, the better diffusibility of sdAb-QD nanoprobes through tissues facilitates the access to small metastases and complex structures, making them powerful tools for cancer diagnostic and therapeutic applications [9]. …”

Section: Resultsmentioning

confidence: 99%

Quantum Dot Conjugates in Functional Imaging and Highly Sensitive Biochemical Assays

Nabiev¹

2018

KEn

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Semiconductor quantum dots (QDs) are characterized by orders of magnitude higher multiphoton linear absorption cross-sections compared with conventional organic dyes. Combined with the QD photoluminescence quantum yield approaching 100%, this fact opens new prospects for the two-photon functional imaging of QDs tagged with highly specific recognition molecules, thus permitting high-quality images with a very low autofluorescence contribution to be obtained. Additionally, unique photostability of QDs enables signal accumulation and significant enhancement of the sensitivity of routine biochemical and immunohistochemical assays to be obtained when the conjugates of QDs, instead of organic dyes, are used.

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“…QDs and nanocrystal-encoded microbeads coupled with antibodies against tumour markers can be useful tools for ultra-sensitive micrometastases detection in serum and tissues [94]. Single domain antibodies-quantum dot conjugates, being much smaller in size compared to similar conventional antibody-based systems, hence offering a deeper tissue penetration in clinical biopsy samples, have been shown to be promising candidates for detection of lung and breast cancer biomarkers [95].…”

Section: Quantum Dots For Diagnostics and Therapymentioning

confidence: 99%

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Volkov

2015

Biochemical and Biophysical Research Communications

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a b s t r a c tThe review addresses the current state of progress in the use of ultra-small nanoparticles from the category of quantum dots (QDs), which presently embraces a widening range of nanomaterials of different nature, including "classical" semiconductor groups III-V and II-VI nanocrystals, along with more recently emerged carbon, silicon, gold and other types of nanoparticles falling into this class of nanomaterials due to their similar physical characteristics such as small size and associated quantum confinement effects. A diverse range of QDs applications in nanomedicine has been extensively summarised previously in numerous publications. Therefore, this review is not intended to provide an all-embracing survey of the well documented QDs uses, but is rather focused on the most recent emerging developments, concepts and outstanding unresolved problematic and sometimes controversial issues. Over 125 publications are overviewed and discussed here in the context of major nanomedicine domains, i.e. medical imaging, diagnostics, therapeutic applications and combination of them in multifunctional theranostic systems.© 2015 Elsevier Inc. All rights reserved. Quantum dots in nanomedicine: recent trendsWithin a steadily increasing database of diverse nanomaterials reported as suitable for applications in nanomedicine [1e10], quantum dots (QDs) deservedly occupy a special niche as nanoparticles with a unique track record full of high expectations, dramatic pitfalls and often controversial experimental evidence. From the early optimistic aspirations of them as breakthrough tools for multipurpose in vitro and in vivo applications [11e17], they have been subject to a period of partial relinquishment following the sombre realisation that in the original unmodified state QDs did not stand a chance to deserve a unanimous approval as imaging or drug delivery vehicles in humans, due to their intrinsic toxicity and lack of biodegradability perspective. The disappointingly low yield of exploitable outputs following the initial rounds of heavy investments in the field has also contributed to the overall decline in research productivity metrics. The analysis of the recent ten years' trends in publications number in this area related to the QDs medical applications in vivo in general (Fig. 1 A), as well as for imaging and diagnostics purposes (Fig. 1 B, C) according to Thomson Reuters Web of Science™ database clearly reflects such "cooling down" period after 2010 resulting in a significant reduction in the initially steady exponential growth of publication rates. This trend has been partially or completely reversed by 2014 and it is intriguing whether we will see it sustained in 2015 and beyond.Such returning enthusiasm has been reinvigorated by a number of objective tendencies in the related technological developments, including the arrival of innovative nanotechnology-enabled tools for diagnostic and ex vivo imaging applications [18,19], the arrival of new types of QDs of alternative nature offering the opportunities of r...

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Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

Cited by 90 publications

References 62 publications

Cancer biomarker detection: recent achievements and challenges

Cancer biomarker detection: recent achievements and challenges

Quantum Dot Conjugates in Functional Imaging and Highly Sensitive Biochemical Assays

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

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