Highly sensitive quantification for human plasma-targeted metabolomics using an amine derivatization reagent

Arashida, Naoko; Nishimoto, Rumi; Harada, Masashi; Shimbo, Kazutaka; Yamada, Naoyuki

doi:10.1016/j.aca.2016.11.068

Cited by 18 publications

(12 citation statements)

References 52 publications

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“…The plasma samples were deproteinized using acetonitrile at a final concentration of 80% before the measurements. The concentrations of the plasma amino acids and their related metabolites were measured using high‐performance liquid chromatography‐electrospray ionization mass spectrometry, followed by pre‐column derivatization using previously described analytical methods …”

Section: Methodsmentioning

confidence: 99%

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Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Adachi

Toyoshima

Nishimoto

et al. 2018

Geriatrics Gerontology Int

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Aim To examine the association between depressive symptoms and plasma amino acid related metaboli in older adults. Methods A total of 152 older adults aged ≥65 years, residing in Niigata, Japan, were used for analysis. We evaluated depressive symptoms using the Geriatric Depression Scale‐15, which has been validated in older community‐dwelling individuals, and used a cut off score of ≥5 to classify participants as having depressive symptoms. We used high‐performance liquid chromatography‐electrospray ionization mass spectrometry to measure the concentrations of plasma amino acid‐related metabolites, and carried out logistic regression analysis to assess the association between depressive symptoms and plasma amino acid‐related metabolites. Results Of the 119 older adults (mean age 76.3 years) included in the analysis, 22 were classified as having depressive symptoms (depressive group). There were no significant differences in physical and cognitive impairments between participants in the depressive and non‐depressive groups. The plasma α‐aminobutyric acid (AABA) level was significantly lower in the depressive group than in the non‐depressive group (P < 0.001). Logistic regression analysis showed the best‐fit model, which included AABA, leucine, threonine, hydroxyl proline and histidine levels (area under the receiver operating characteristic curve 0.8346; 95% confidence interval 0.7365–0.9326). In particular, the plasma AABA level was strongly associated with depressive symptoms. Conclusions Plasma AABA level is significantly associated with depression symptoms in older community‐dwelling adults in Japan. Thus, plasma AABA might serve as a potential marker of depression in older adults aged ≥65 years. Geriatr Gerontol Int 2019; 19: 254–258.

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Section: Methodsmentioning

confidence: 99%

“…The concentrations of the plasma amino acids and their related metabolites were measured using high-performance liquid chromatography-electrospray ionization mass spectrometry, followed by pre-column derivatization using previously described analytical methods. [21][22][23]…”

Section: Geriatric Depressive Symptomsmentioning

confidence: 99%

Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Adachi

Toyoshima

Nishimoto

et al. 2018

Geriatrics Gerontology Int

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“…Tryptophan was analyzed and quantified using LC‐MS/MS (Agilent 1290 Infinity series liquid chromatography system; Agilent Technologies) coupled to an API4000 mass spectrometer (AB Sciex). Kynurenine, 5‐HTP, and serotonin were analyzed and quantified using LC‐MS/MS (ACQUITY UPLC system; Waters) coupled to a 5500 QTRAP mass spectrometer (AB Sciex) as described previously …”

Section: Methodsmentioning

confidence: 99%

Induction of tryptophan hydroxylase in the liver of s.c. tumor model of prostate cancer

et al. 2020

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Enhanced degradation of tryptophan (Trp) and thus decreased plasma Trp levels are common in several types of cancers. Although it is well known that Trp catabolism is induced in the tumor microenvironment by the enzymes expressed in cancer cells, immune cells, or both, few studies have examined systemic Trp catabolism in cancer pathophysiology. The present study aimed to evaluate Trp catabolism in both tumor and peripheral tissues using tumor-engrafted Copenhagen rats that were s.c. inoculated with AT-2 rat prostate cancer cells negative for expression of Trp catabolic enzymes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics showed significantly decreased plasma Trp levels in AT-2 engrafted rats, accompanied by increased kynurenine/Trp ratios in spleen and thymus and serotonin levels in liver and thymus. Quantitative PCR and enzymatic activity assays showed indoleamine-2, 3-dioxygenase, an inducible enzyme that catalyzes Trp to kynurenine, was increased in tumor tissues, whereas tryptophan-2,3-dioxygenase, a major Trp catabolic enzyme that regulates systemic level of Trp, tended to be increased in the liver of AT-2 engrafted rats. Furthermore, tryptophan hydroxylase-1 (TPH1), an enzyme that catalyzes the reaction of Trp to serotonin, was significantly increased in liver and spleen of AT-2 engrafted rats. Further histochemical analysis revealed that the induction of TPH1 in the liver could be attributed to infiltration of mast cells. A similar phenomenon was observed with nonneoplastic liver samples from colorectal cancer patients. These results suggested that Trp catabolism toward serotonin synthesis might be induced in peripheral remote tissues in cancer, which could have a pathophysiological effect on cancer. K E Y W O R D S liver, mast cell, serotonin, tryptophan, tryptophan hydroxylase | 1219 HAGIWARA et Al. S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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“…The concentrations of Trp and ILA in human plasma were found to be much higher than those of other metabolites, moreover, their peak intensities were saturated. To solve this problem, we selected the isotopic ion (m+1) of Trp and ILA for determination [25,27]. The extracted mass chromatograms under optimized analytical condition are shown in Fig.…”

Section: Optimization Of Lc-ms/ms Conditionsmentioning

confidence: 99%

“…Various methods of analyzing Trp metabolites have been reported [17][18][19][20][21][22][23][24]. In our previous studies, we have developed analytical methods to quantify amino acids and their metabolites in human plasma using pre-column derivatization with a 3-aminopyridyl-N-hydroxy succinimidyl carbamate (APDS) reagent and liquid chromatography tandem mass spectrometry (LC-MS/MS) [25]. However, some Trp metabolites could not be analyzed using the APDS reagent because of their lack of amino groups.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

et al. 2019

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A simplified method for analyzing tryptophan (Trp) and its metabolites in human plasma was developed using liquid chromatography-electrospray ionization tandem mass spectrometry. Trp and its metabolites have various chemical properties but have no common functional group for derivatization. Using a reversed-phase pentafluorophenyl (PFP) column for liquid chromatography separation, Trp and its 15 metabolites (3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, 3-indoleacetic acid, 5-hydroxyindole-3-acetic acid, 5-hydroxy-L-tryptophane, anthranilic acid, indole-3-lactic acid, kynurenine, kynurenic acid, melatonin, nicotinic acid, picolinic acid, quinolinic acid, serotonin, and xanthurenic acid were successfully separated within 15 min without derivatization. Neopterin which is known as a biomarker for inflammation and is often evaluated with Trp metabolites in several reports could also be simultaneously analyzed. With this method, Trp and its metabolites were detected with good sensitivity and selectivity without derivatization and solid-phase extraction. The method was validated in this study, showing that the relative standard deviation of 14 Trp metabolites was <15%, and accuracy was within 100% ± 20%, with the exception of nicotinic acid. The quantification range was optimized to 0.0150-100 µM covering the concentration of Trp metabolites in human plasma. Overall, 12 Trp metabolites in healthy human plasma were quantified with good precision and accuracy.

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Highly sensitive quantification for human plasma-targeted metabolomics using an amine derivatization reagent

Cited by 18 publications

References 52 publications

Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Induction of tryptophan hydroxylase in the liver of s.c. tumor model of prostate cancer

Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

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