Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

Hurot, Charlotte; Brenet, Sophie; Buhot, Arnaud; Barou, Emilie; Belloir, Christine; Briand, Loı̈c; Hou, Yanxia

doi:10.1016/j.bios.2018.08.072

Cited by 49 publications

(29 citation statements)

References 39 publications

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“…SPRi has been largely used for the development of biochips for monitoring biomolecular binding events [18][19][20] as well as the development of electronic tongues for the analysis of proteins [22] and complex mixtures in liquid [23] [24] [25]. Recently, we have demonstrated its efficiency for the development of olfactory biosensors using OBPs [26]. Furthermore, in another study, for the first time, we have proven that SPRi is very efficient for the development of the eNs for sensing VOCs in gas phase [27].…”

Section: Introductionmentioning

confidence: 99%

Development of an optoelectronic nose based on surface plasmon resonance imaging with peptide and hairpin DNA for sensing volatile organic compounds

Gaggiotti

Hurot

Weerakkody

et al. 2020

Sensors and Actuators B: Chemical

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Nowadays, the analysis of volatile organic compounds (VOCs) is very important in various domains. For this, in the last decades, electronic noses have emerged as promising alternatives to traditional analytical methods. Nevertheless, their wide use is still limited by their performances such as low selectivity. Herein, we developed an optoelectronic nose using virtually screened peptides and hairpin DNA (hpDNA) with improved selectivity as sensing materials and surface plasmon resonance imaging (SPRi) as the detection system. Thanks to the complementarity of their binding properties towards target VOCs, the obtained optoelectronic nose has very good selectivity, being able to discriminate not only between VOCs of different chemical families, but also VOCs of the same family with only 1-carbon difference. We thus confirmed that computational virtual screening, which allows 'in silico' testing of VOC-peptide binding in a fast and low-cost way, is very promising for the selection of sensing elements with higher sensitivity and selectivity as well as great diversity. The combination of these sensing materials with SPRi is relevant for the development of optoelectronic nose with large sensor arrays and improved performances.

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Section: Introductionmentioning

confidence: 99%

Development of an optoelectronic nose based on surface plasmon resonance imaging with peptide and hairpin DNA for sensing volatile organic compounds

Gaggiotti

Hurot

Weerakkody

et al. 2020

Sensors and Actuators B: Chemical

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“…Biological olfaction uses a wider variety of sensing materials (up to 1,000 receptor subtypes, although all may not be used, see [23]) and further development should produce new sensing materials which can be immobilized on the surface to increase the number of olfactory receptors (see [11] for the use of odorant binding proteins and [10] for the use of hairpin DNA). Further studies should be conducted on a wider range of chiral pairs, but also on enantiomeric mixtures.…”

Section: Resultsmentioning

confidence: 99%

Reliable chiral recognition with an optoelectronic nose

Maho

Herrier²,

Livache³

et al. 2020

Biosensors and Bioelectronics

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Chiral discrimination is a key problem in analytical chemistry. It is generally performed using expensive instruments or highly-specific miniaturized sensors. An electronic nose is a bio-inspired instrument capable after training of discriminating a wide variety of analytes. However, generality is achieved at the cost of specificity which makes chiral recognition a challenging task for this kind of device. Recently, a peptide-based optoelectronic nose which can board up to hundreds of different sensing materials has shown promising results, especially in terms of specificity. In line with these results, we describe here its use for chiral recognition. This challenging task requires care, especially in terms of statistical reliability and experimental confounds. For these reasons, we set up an automatic gas sampling system and recorded data over two long sessions, taking care to exclude possible confounds. Two couples of chiral molecules, namely (R) and (S) Limonene and (R) and (S) Carvone, were tested and several statistical analyses indicate the almost perfect discrimination of their two enantiomers. A method to highlight discriminative sensing materials is also proposed and shows that successful discrimination is likely achieved using just a few peptides.

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“…They bind VOCs with affinities in the micromolar range, with relatively low specificity. However, it is possible to tune their binding properties by site-directed mutagenesis [37]. Insect OBPs have a more rigid structure composed of six α-helices stabilized with three disulfide bridges [38].…”

Section: Bio-sourced Materialsmentioning

confidence: 99%

“…In the literature, various ingenious strategies have been developed for this purpose. OBPs can be immobilized using a cysteine [37] or histidine tag [96,113] at their N-terminus so as to keep a good access of the VOCs to the binding pocket. Kotlowski et al [114], Larisika et al [114], and Zhang et al [115] proposed a solution with a bi-functional linker (Figure 7f).…”

Section: Increase Of the Sensitivitymentioning

confidence: 99%

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Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing materials, their immobilization on the sensing surface, the sampling of VOCs, the choice of a transduction method, and the data processing. This reflection could help address new applications in domains where high-performance artificial noses are required such as public security and safety, environment, industry, or healthcare.

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Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

Cited by 49 publications

References 39 publications

Development of an optoelectronic nose based on surface plasmon resonance imaging with peptide and hairpin DNA for sensing volatile organic compounds

Development of an optoelectronic nose based on surface plasmon resonance imaging with peptide and hairpin DNA for sensing volatile organic compounds

Reliable chiral recognition with an optoelectronic nose

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

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