Highly sensitive fluorescent detection of p53 protein based on DNA functionalized Fe3O4 nanoparticles

Xu, Qingsong; Liang, Kaixin; Liu, Ren-Yu; Liu, Deng; Zhang, Min; Shen, Liangfang; Liu, You-Nian

doi:10.1016/j.talanta.2018.05.009

Cited by 20 publications

(10 citation statements)

References 28 publications

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“…In general, aptamers and ribozymes are two types of nucleic acid recognition elements for non-nucleic acid biomarkers. In this section, we mainly introduce nucleic acid recognition elements for the detection of small molecules, ,,− proteins, ,,− cells, and metal ions ,− based on aptamers and ribozymes (Figure ).…”

Section: Clinical Translation-driven Nucleic Acid-based Testsmentioning

confidence: 99%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are natural biopolymers composed of nucleotides that store, transmit, and express genetic information. Overexpressed or underexpressed as well as mutated nucleic acids have been implicated in many diseases. Therefore, nucleic acid tests (NATs) are extremely important. Inspired by intracellular DNA replication and RNA transcription, in vitro NATs have been extensively developed to improve the detection specificity, sensitivity, and simplicity. The principles of NATs can be in general classified into three categories: nucleic acid hybridization, thermal-cycle or isothermal amplification, and signal amplification. Driven by pressing needs in clinical diagnosis and prevention of infectious diseases, NATs have evolved to be a rapidly advancing field. During the past ten years, an explosive increase of research interest in both basic research and clinical translation has been witnessed. In this review, we aim to provide comprehensive coverage of the progress to analyze nucleic acids, use nucleic acids as recognition probes, construct detection devices based on nucleic acids, and utilize nucleic acids in clinical diagnosis and other important fields. We also discuss the new frontiers in the field and the challenges to be addressed.

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Section: Clinical Translation-driven Nucleic Acid-based Testsmentioning

confidence: 99%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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“…Another example of spectrometric detection in biosensor systems is fluorescence registration. A fluorescent biosensor for p53 protein quantification was explored using DNA/dextran/PAA/Fe 3 O 4 nanocarriers by Xu et al [ 76 ]. Dextran-aminated MNPs were used to functionalize the consensus DNA that can selectively bind wild-type p53 protein ( Figure 6 ).…”

Section: Synthetic Magnetic Nanoparticlesmentioning

confidence: 99%

“…The sensor can realize ultrasensitive detection of p53 protein in real cell lysate. Reprinted from [ 76 ] with permission of Elsevier provided by Copyright Clearance Center.…”

Section: Figurementioning

confidence: 99%

Biosensors and Drug Delivery in Oncotheranostics Using Inorganic Synthetic and Biogenic Magnetic Nanoparticles

et al. 2022

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Magnetic nanocarriers have attracted attention in translational oncology due to their ability to be employed both for tumor diagnostics and therapy. This review summarizes data on applications of synthetic and biogenic magnetic nanoparticles (MNPs) in oncological theranostics and related areas. The basics of both types of MNPs including synthesis approaches, structure, and physicochemical properties are discussed. The properties of synthetic MNPs and biogenic MNPs are compared with regard to their antitumor therapeutic efficiency, diagnostic potential, biocompatibility, and cellular toxicity. The comparative analysis demonstrates that both synthetic and biogenic MNPs could be efficiently used for cancer theranostics, including biosensorics and drug delivery. At the same time, reduced toxicity of biogenic particles was noted, which makes them advantageous for in vivo applications, such as drug delivery, or MRI imaging of tumors. Adaptability to surface modification based on natural biochemical processes is also noted, as well as good compatibility with tumor cells and proliferation in them. Advances in the bionanotechnology field should lead to the implementation of MNPs in clinical trials.

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“…Xu and co-workers designed a DNA-based MNPs (DNA/dextran/PAA/Fe 3 O 4 NPs) sensor working as a signal-off fluorescent assay for the sensitive determination of p53 protein expression [55]. The fluorescent sensor showed a dose-response in the linear range from 50 pM to 2 nM and detected p53 low to 8 pM (LOD).…”

Section: Biosensingmentioning

confidence: 99%

“…The combination of the PEC enhanced features of the ZnIn 2 S 4 /ZnO-NRs/indium tin oxide photoelectrode and the highly effective his-Fe 3 O 4 @nanozyme enabled the determination of PSA down to fg/mL range (LOD = 18 fg/mL). Xu and co-workers designed a DNA-based MNPs (DNA/dextran/PAA/Fe3O4 NPs) sensor working as a signal-off fluorescent assay for the sensitive determination of p53 protein expression [55]. The fluorescent sensor showed a dose-response in the linear range from 50 pM to 2 nM and detected p53 low to 8 pM (LOD).…”

Section: Biosensingmentioning

confidence: 99%

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

2019

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During the last few decades, magnetic nanoparticles have been evaluated as promising materials in the field of cancer detection, screening, and treatment. Early diagnosis and screening of cancer may be achieved using magnetic nanoparticles either within the magnetic resonance imaging technique and/or sensing systems. These sensors are designed to selectively detect specific biomarkers, compounds that can be related to the onset or evolution of cancer, during and after the treatment of this widespread disease. Some of the particular properties of magnetic nanoparticles are extensively exploited in cancer therapy as drug delivery agents to selectively target the envisaged location by tailored in vivo manipulation using an external magnetic field. Furthermore, individualized treatment with antineoplastic drugs may be combined with magnetic resonance imaging to achieve an efficient therapy. This review summarizes the studies about the implications of magnetic nanoparticles in cancer diagnosis, treatment and drug delivery as well as prospects for future development and challenges of magnetic nanoparticles in the field of oncology.

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Highly sensitive fluorescent detection of p53 protein based on DNA functionalized Fe3O4 nanoparticles

Cited by 20 publications

References 28 publications

Nucleic Acid Tests for Clinical Translation

Nucleic Acid Tests for Clinical Translation

Biosensors and Drug Delivery in Oncotheranostics Using Inorganic Synthetic and Biogenic Magnetic Nanoparticles

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

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