Highly sensitive fluorescent aptasensor based on MoS2 nanosheets for one-step determination of methamphetamine

Yao, Wenjie; Jiang, Ling; Zhang, Wenqi; Ding, Yanjun

doi:10.2116/analsci.21p144

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…Fluorescence analysis is an advanced analytical method with a high sensitivity, selectivity, and practicality, which can qualitatively and quantitatively analyse the changes in fluorescence intensity, emission spectrum, and fluorescence molecular lifetime of substances [ 121 ]. Due to the strong adsorption capacity and wide absorption spectrum of MoS 2 NSs to ssDNA, MoS 2 NSs can quench fluorescent groups with different emission wavelengths, showing unique advantages in the construction of fluorescent biosensor platforms [ 122 ]. Liang et al [ 123 ] built a fluorescence sensing platform for the hepatocellular carcinoma (HCC) biomarker GP73 based on the Förster resonance energy transfer (FRET).…”

Section: Optical Biosensors For Cancer Biomarker Detection Based On Mosmentioning

confidence: 99%

Molybdenum Disulfide as Tunable Electrochemical and Optical Biosensing Platforms for Cancer Biomarker Detection: A Review

Qin,

Zhang,

2023

Biosensors

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Cancer is a common illness with a high mortality. Compared with traditional technologies, biomarker detection, with its low cost and simple operation, has a higher sensitivity and faster speed in the early screening and prognosis of cancer. Therefore, extensive research has focused on the development of biosensors and the construction of sensing interfaces. Molybdenum disulfide (MoS2) is a promising two-dimensional (2D) nanomaterial, whose unique adjustable bandgap shows excellent electronic and optical properties in the construction of biosensor interfaces. It not only has the advantages of a high catalytic activity and low manufacturing costs, but it can also further expand the application of hybrid structures through different functionalization, and it is widely used in various biosensors fields. Herein, we provide a detailed introduction to the structure and synthesis methods of MoS2, and explore the unique properties and advantages/disadvantages exhibited by different structures. Specifically, we focus on the excellent properties and application performance of MoS2 and its composite structures, and discuss the widespread application of MoS2 in cancer biomarkers detection from both electrochemical and optical dimensions. Additionally, with the cross development of emerging technologies, we have also expanded the application of other emerging sensors based on MoS2 for early cancer diagnosis. Finally, we summarized the challenges and prospects of MoS2 in the synthesis, functionalization of composite groups, and applications, and provided some insights into the potential applications of these emerging nanomaterials in a wider range of fields.

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Section: Optical Biosensors For Cancer Biomarker Detection Based On Mosmentioning

confidence: 99%

Molybdenum Disulfide as Tunable Electrochemical and Optical Biosensing Platforms for Cancer Biomarker Detection: A Review

Qin,

Zhang,

2023

Biosensors

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“…These methods could supply accurate results of MAMP in complex samples including human urine and blood. However, they have several drawbacks, such as expensive instruments, complex sample preparation and not suitable for real-time detection [11][12][13][14][15] . Therefore, it's very necessary to set up a simple, accurate and fast method for the detection of MAMP.…”

Section: Introductionmentioning

confidence: 99%

A signal-on electrochemical aptasensor for rapid detection of methylamphetamine based on competition with complementary DNA

Chang,

Zheng,

et al. 2023

Preprint

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A simple and rapid electrochemical sensing method with high sensitivity and specificity of aptamers was developed for the detection of methylamphetamine (MAMP).A short anti-MAMP thiolated aptamer (Apt) with a methylene blue (MB) probe at 3’-end was immobilized on the surface of a gold electrode (MB-Apt-S/GE). The electrochemical signal appeared when MAMP presenting in the sample solution competed with cDNA for binding with MB-Apt-S. Under optimized conditions, the liner range of this signal-on electrochemical aptasensor for the detection of MAMP achieved from 1.0 to 10.0 nmol/L and 10.0 to 400 nmol/L. Satisfactory spiked recoveries of saliva and urine were also obtained. In this method, only 5 minutes were needed to incubate before the square wave voltammetry (SWV) analysis, which was much more rapid than other electrochemical sensors, leading to a bright and broad prospect for the detection of MAMP in biological sample. This method can be used for on-site rapid detection on special occasions, such as drug driving scenes, entertainment venues suspected of drug use, etc.

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Aptamer‐based fluorescent sensor for highly sensitive detection of methamphetamine

Wang,

Tong

et al. 2024

Luminescence

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The construction of a fluorescence aptamer sensor was achieved by employing the fundamental principle of fluorescence resonance energy transfer. By employing molecular modeling technologies to identify the binding site, the high‐affinity aptamer APT‐40nt was derived from the whole sequence and utilized on the graphene oxide (GO) fluorescent platform for the purpose of achieving a highly sensitive detection of methamphetamine (METH). The aptamer tagged with fluorescein (FAM) dye undergoes quenching in the presence of GO due to π‐stacking interaction. With the addition of the target, the aptamer that has been tagged was detached from the GO surface, forming a stable complex with METH. This process resulted in fluorescence restoration of the system, and the degree of fluorescence restoration was proportional to METH concentration in the linear range of 1–50 and 50–200 nM. Notably, under optimized conditions, the detection limit of this aptasensor was as low as 0.78 nM, which meets the detection limit requirements of METH detection in saliva and urine in some countries and regions. Moreover, other common illicit drugs and metabolites had minimizing interference with the determination. The established aptasensor, therefore, has been successfully applied to detect METH in saliva and urine samples and exhibited satisfactory recoveries (87%–111%). This aptasensor has the advantages of low detection limit, excellent selectivity, ease of operation, and low cost, providing a promising strategy for on‐site detection of METH in saliva and urine.

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Highly sensitive fluorescent aptasensor based on MoS2 nanosheets for one-step determination of methamphetamine

Cited by 8 publications

References 28 publications

Molybdenum Disulfide as Tunable Electrochemical and Optical Biosensing Platforms for Cancer Biomarker Detection: A Review

Molybdenum Disulfide as Tunable Electrochemical and Optical Biosensing Platforms for Cancer Biomarker Detection: A Review

A signal-on electrochemical aptasensor for rapid detection of methylamphetamine based on competition with complementary DNA

Aptamer‐based fluorescent sensor for highly sensitive detection of methamphetamine

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