Highly sensitive fluorescence biosensing of BCR-ABL1 fusion gene based on exponential transcription-triggered hemin catalysis

CATCH: high specific transcriptome-focused fusion gene variants discrimination

“…S5 and Table S3, ESI†). 14,21–28 Furthermore, among the mixed samples with different concentrations of RNA targets, we observed that the LOD of the samples containing EML4-ALK V1 in 10 ng total RNA was 0.5% (Fig. 2D).…”

“…Fig.2A-Cshowed a good linear relationship between the peak of fluorescence spectra and input concentrations (R 2 = 0.993). The proposed CATCH approach enabled EML4-ALK V1 detection in the femtogram range, which has been improved by four orders of magnitude compared with the CATCH assay without subsequent CHA amplification, and also had a better analytical performance than other methods (Fig.S5and TableS3, ESI †) 14,[21][22][23][24][25][26][27][28]. Furthermore, among the mixed samples with different concentrations of RNA targets, we observed that the LOD of the samples containing EML4-ALK V1 in 10 ng total RNA was 0.5% (Fig.2D).We also evaluated the detection capability of the proposed approach by a standard recovery experiment and repeatability study.…”

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

“…As a benefit from this, the interference of non-fluorescent components can be considerably avoided, which is the optimum choice for most trace target analysis ( Casto-Boggess et al, 2022 ). Traditional fluorescence detection methods mostly use ready-made peroxidases such as HRP ( Lu and Xu, 2019 ; Heo et al, 2021 ) and ALP ( Han et al, 2020 ; Wu et al, 2022 ) to catalyze the substrate such as tyramine ( Zhang et al, 2020a ; Kang et al, 2020 ) and thioflavin T ( Wu et al, 2018 ; Pramanik et al, 2021 ) to output fluorescent signals. The disadvantages of this method lie in that the enzyme protein is relatively unstable and has harsh requirements on reaction conditions, which make researchers turn their attention to the G4-hemin biomimetic enzyme ( Li et al, 2021a ; Huang et al, 2021 ).…”