Highly sensitive flow-injection determination of glucose in plasma using an immobilized pyranose oxidase and a chemiluminometric peroxidase sensor

Kiba, Nobutoshi; Itagaki, Akiko; Fukumura, Satoru; Saegusa, Kazuya; Furusawa, Motohisa

doi:10.1016/s0003-2670(97)00442-x

Cited by 24 publications

(13 citation statements)

References 23 publications

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“…When not in use, the immobilized POx flow cell was stored under the same conditions as described in previous paper. 11 The present method was compared with HPLC. 2 Fish meat (2 g) was suspended in a phosphate buffer (20 ml), milled mechanically and centrifuged (13000g) for 15 min at 4˚C.…”

Section: Flow System and Proceduresmentioning

confidence: 99%

Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

et al. 2006

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Section: Flow System and Proceduresmentioning

confidence: 99%

Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

et al. 2006

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“…27 The tresyalte-beads were suspended in 5 ml of 0.1 M phosphate buffer (pH 7.0) containing ChOx (2 mg), POx (2 mg), AChE (2 mg) or ChOx/POx (2 mg:1 mg) at ambient temperature (25 ± 2˚C) for 2 h with occasional shaking. During the immobilization process for ChOx, the absorbance at 280 nm was monitored.…”

Section: Preparation Of Immobilized Enzymesmentioning

confidence: 99%

Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System

et al. 2003

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The development of methods for rapid and sensitive determination of neurotransmitters and their precursors and/or metabolites has been a challenging goal for many years since they provide valuable information for disposition dynamics rather than the static levels of neurotransmitters. Acetylcholine (ACh), a main neurotransmitter in both the peripheral and central nervous systems, is found in mammalian brain tissues. Despite intensive research, the roles of neurotransmitters in the central nervous system are much less delineated, although it is generally considered that ACh is involved in temperature and blood pressure regulation, motor coordination, learning, memory, etc.1 Furthermore, ACh and choline (Ch) levels in the brain are very important due to the significant role in the neuropsychiatric diseases; 2-6 Ch serves both as the precursor and metabolite of ACh.Numerous chromatographic methods have been described for the simultaneous measurement of Ch and ACh. Though high performance liquid chromatography (HPLC) coupled with MS or with tandem MS required minimal sample preparation and no derivatization, it had relatively inadequate quantitation limits 9-12 HPLC-electrochemical detection (ECD) with immobilized acetylcholinesterase (EC 3.1.1.7, AChE) and Ch oxidase (EC 1.1.3.7, ChOx) reactors as column reactor 13,14 or with coimmobilized AChE/ChOx bienzyme electrode 15 were suited for the sensitive determinations of Ch and ACh; the detection limits and sample throughput have been reported to be 1 and 3 nM for Ch and ACh, respectively, and 3/h. 15 The slower flow rate is preferable to the conversion efficencies for the analytes to H2O2 and to the sensitive detection of the H2O2, but the decreased sample throughput is undesirable to follow minutely the dynamics of ACh and Ch.For the rapid assays, though there have been many flow injection (FI) methods with immobilized ChOx or coimmobilized AChE/ChOx for the determination of Ch or ACh, 16-20 a split-steam FI system with chemiluminometric detection using three immobilized enzyme column reactors such as an immobilized ChOx, an immobilized catalase and a coimmobilized AChE/ChOx, and using ferricyanide-catalyzed luminol reaction have been developed for simultaneous determination of Ch and ACh. 21In a split-stream FI system with column reactors, generally, sample plugs from the reactors disperse into the carrier stream and are, moreover, diluted with the carrier solution from other streams; the resulting peak broadening causes lowering of sensitivity and sample throughput. Flow-through sensors based on the integration of conversion reaction and detection reaction in a flow cell have been designed to solve the problems. [22][23][24] We have recently proposed chemiluminometric biosensors for simultaneous determination of oxidase substrates on the basis of integration of oxidase reactions and peroxidase (POx)-catalyzed H2O2/luminol detection reactions, which involved no separation process. 25,26 This paper describes the performance of a rapid and sensitive flow-through sen...

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“…The tube was coiled spirally and used as a flow cell. 22,23 The immobilized enzymes were washed with 0.1 M Tris-HCl buffer (pH 8.0) to saturate the free linking sites.…”

Section: Construction Of the Immobilized Enzyme Flow Cellmentioning

confidence: 99%

Flow-through Chemiluminescence Sensor Using Immobilized Oxidases for the Selective Determination of l-Glutamate in a Flow-Injection System

et al. 2001

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A selective and sensitive chemiluminometric flow sensor for the determination of L-glutamate in serum, based on immobilized oxidases such as glutamate oxidase (GOD), uricase (UC) and peroxidase (POD), is described herein. The principle for the selective chemiluminometric detection for L-glutamate is based on coupled reactions of four sequentially aligned immobilized oxidases, UC/POD/GOD/POD in a flow cell. The immobilized UC was employed to decompose urate, which is one of the major interfering components in serum for a luminol-H2O2 chemiluminescence reaction. The H2O2 produced from the UC reaction readily reacted with reducing components, such as ascorbate and glutathione, and then the excess H2O2 was decomposed by the immobilized POD. L-Glutamate in the sample plug was enzymatically converted to H2O2 with immobilized GOD. Subsequently, the peroxide reacts with luminol on the immobilized POD to produce chemiluminescenece, proportional to glutamate concentration. The enzymes were immobilized on tresylated poly(vinyl alcohol beads). The immobilized enzymes were packed into a TPFE tube (1.0 mm i.d. × 60 cm), in turn, and used as a flow cell. The sampling rate was 30 h -1 . The calibration graph for L-glutamate is linear for 20 nM -5 µM; the detection limit (signal-to-noise = 3) is 10 nM.

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Highly sensitive flow-injection determination of glucose in plasma using an immobilized pyranose oxidase and a chemiluminometric peroxidase sensor

Cited by 24 publications

References 23 publications

Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection

Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System

Flow-through Chemiluminescence Sensor Using Immobilized Oxidases for the Selective Determination of l-Glutamate in a Flow-Injection System

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