Highly sensitive double‐fluorescent dye staining on microchip electrophoresis for analysis of milk proteins

Okada, Hiroki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu

doi:10.1002/elps.200700775

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…Okada et al. used a highly sensitive double‐fluorescent dye staining and analyzed the proteins in a real sample of cow's milk by ME [97]. Similarly to what is achieved by Del Mar Barrios‐Romero et al.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

Authenticity assessment of dairy products by capillary electrophoresis

et al. 2021

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Authenticity assessment of dairy products by capillary electrophoresisMilk and derivatives are a very important part in the diet of the world population. Products from goat, buffalo, and sheep species have a greater economic value than the cow ones, therefore, authenticity frauds by improperly adding cow's milk occur frequently: dairy products are among the seven more attractive foods for adulteration. Milk from each of the above-cited animal species has its own definite profile of whey proteins (variants of α-lactalbumin and β-lactoglobulin) and its definite profile of caseins (variants of α S1 -, α S2 -, β-, and κ-casein). Such proteins can be usefully exploited as markers of authenticity by using capillary electrophoresis which is the technique of choice for the analysis of proteins. Due to the multiple adjustable parameters that are unknown to other analytical techniques, capillary electrophoresis is able to detect frauds in milk mixtures and cheese with little use of solvents, fast analysis time, and ease of operation. This makes it attractive and competitive for routine checks that are very important to fight the adulteration market. Advantages and limitations are discussed.

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Section: Capillary Electrophoresismentioning

confidence: 99%

Authenticity assessment of dairy products by capillary electrophoresis

et al. 2021

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“…A highly sensitive double‐fluorescent dye staining in ME for analysis of milk proteins has also been reported . Firstly, before the electrophoresis method; the proteins extracted from the sample were covalently bound to an amine‐reactive fluorescent dye, Cy5.…”

Section: Optical Detection On Microchips Electrophoresismentioning

confidence: 99%

“…BSA (68 kDa) was used. Each arrow shows the concentration of BSA and insets (magnified electropherograms) are available for easy observation and even have detectable peaks (S/N .3) (Reprinted with permission from; Copyright 2008 John Wiley and Sons).…”

Section: Optical Detection On Microchips Electrophoresismentioning

confidence: 99%

Food analysis on microchip electrophoresis: An updated review

2012

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From 2008 to date, basically, single‐cross microchip electrophoresis (ME) design has been used for food analysis with electrochemicaland laser‐induced fluorescencedetection being the most commonprinciples coupled. In the last 4 years, the main outlines were: (i) the exploration of new analytes such as heavy metals, nitrite, micotoxins, microorganisms, and allergens; (ii) the development of electrokinetic microfluidic (bio‐) sensors into microchip format for the detection of toxins; and interestingly (iii) although sample preparation is still performed off‐chip, an important increase in works dealing with complicated food samples has been clearly noticed. Although microchip technology based on electrokinetics is emerging from important fields such as authentication of foods, detection of frauds, toxics, and allergens; the marriage between micro‐ and nanotechnologies and total integration approaches has not reached the expected impact in the field but it is still a great promise for the development of ME of new generations for food analysis.

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“…Above all, this coupling has been mainly used for protein analysis. It was selected for the determination of amino acids, biogenic amines and proteins in several food stuff, such as sport or fermented beverages and milk 113–115. For example, a quite interesting work was the one developed by Jayarajah et al .…”

Section: Applications Of Miniaturized Techniques In Food Analysismentioning

confidence: 99%

Food analysis: A continuous challenge for miniaturized separation techniques

Asensio-Ramos

Hernández-Borges

Rocco

et al. 2009

J of Separation Science

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One of the current trends of modern analytical chemistry is the miniaturization of the various tools daily used by a large number of researchers. Ultrafast separations, consumption of small amounts of both samples and reagents as well as a high sensitivity and automation are some of the most important goals desired to be achieved. For many years a large number of research laboratories and analytical instrument manufacturing companies have been investing their efforts in this field, which includes miniaturized extraction materials, sample pre‐treatment procedures and separation techniques. Among the separation techniques, capillary electromigration methods (which also include CEC), microchip and nano‐LC/capillary LC have received especial attention. Besides their well‐known advantages over other separation tools, the role of these miniaturized techniques in food analysis is still probably in an early stage. In fact, applications in this field carried out by CEC, microchip, nano‐LC and capillary LC are only a few when compared with other more established procedures such as conventional GC or HPLC. The scope of this review is to gather and discuss the different applications of such miniaturized techniques in this field. Concerning CE, microchip‐CE and CEC works, emphasis has been placed on articles published after January 2007.

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Highly sensitive double‐fluorescent dye staining on microchip electrophoresis for analysis of milk proteins

Cited by 8 publications

References 21 publications

Authenticity assessment of dairy products by capillary electrophoresis

Authenticity assessment of dairy products by capillary electrophoresis

Food analysis on microchip electrophoresis: An updated review

Food analysis: A continuous challenge for miniaturized separation techniques

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