Highly Sensitive Determination for Catecholamines Using Boronate Affinity Polymer Monolith Microextraction with In-Situ Derivatization and HPLC Fluorescence Detection

Fang, Wen-Le; Xia, Lijun; Huang, Xiao; Guo, Xiao‐Feng; Ding, Jun; Wang, Hong; Feng, Yun

doi:10.1007/s10337-018-3592-3

Cited by 27 publications

(3 citation statements)

References 38 publications

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“…The loading buffer contained a sample solution that contained galactose (16 mg mL -1 ) which was pumped using a micropipette. The extraction efficiency is significantly impacted by the sample pH value since the current form of sorbent and analytes are impacted by the sample pH value [63]. Since the boronate affinity chromatography provides for the binding pH buffer to be greater than or equal to the pKa of the boronic acid ligand, which has a moderately low pKa value of AAPBA (8.2 Fig.…”

Section: Capture Of Carbohydrate and Catecholmentioning

confidence: 99%

Organic Boronate Affinity Sorbent for Capture of cis-Diol Containing Compounds Eman Alzahrani

Alzahrani

2019

Asian J. Chem.

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Boronate affinity chromatography (BAC) is argued to be a critical tool in specific capture and separation of cis-diol containing compounds. In present study, organic boronate affinity monolith poly(3-acrylamido phenylboronic acid-co-ethylene dimethacrylate) (AAPBA-co-EDMA) is prepared through one-step in situ polymerization procedure within a micropipette through the application of a pre-polymerization mixture which contains functional monomer (3-acrylamido phenylboronic acid), cross-linker (ethylene dimethacrylate), porogenic solvent (methanol with poly ethylene glycol) and initiator (2,2-dimethoxy-2-phenyl-acetophenone). Following the optimization of time exposure to UV lamp with 365 nm, the macroporous organic boronate monolith was selected. Several approaches including SEM and BET analysis, FT-IR spectroscopy and measuring contact angle were applied in the characterization of the morphology of the monolith. Several cis-diol compounds that include catechol and galactose are applied in the assessment of the boronate affinity of the organic monolithic material. Additionally, the capture of glucose from urine sample is also conducted. The basic principle of the approach is that boronic acid forms covalent bond with cis-diols in basic solutions whereas the ester bonds are dissociated under acidic media. By using the study results, monolith demonstrate good selectivity towards cis-diol containing compounds. Due to the hydrophilic property of monolith, the affinity chromatography monolith can be performed for several cis-diol compounds including glycoproteins and nucleosides. Also, fabrication of the organic boronate monolithic in microfluidic equipment is essential in facilitating the extraction of boronate affinity using small-volume samples.

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Section: Capture Of Carbohydrate and Catecholmentioning

confidence: 99%

Organic Boronate Affinity Sorbent for Capture of cis-Diol Containing Compounds Eman Alzahrani

Alzahrani

2019

Asian J. Chem.

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“…However, large volume of sample and solvent or time-consuming extraction procedures were usually required for them. Polymer monolith micro-extraction (PMME) has gained much attention in sample preparation for various applications [20][21][22]. As a polymer extraction material, poly (methacrylic acid-ethylene glycol dimethacrylate) [poly (MAA-EGDMA)] could provide good biocompatibility for much biological samples, which possessed good permeability to make extraction faster and performed high-throughput analysis.…”

Section: Introductionmentioning

confidence: 99%

“…As a polymer extraction material, poly (methacrylic acid-ethylene glycol dimethacrylate) [poly (MAA-EGDMA)] could provide good biocompatibility for much biological samples, which possessed good permeability to make extraction faster and performed high-throughput analysis. Moreover, good stability in a wide range of pH was suitable for processing biological samples [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Determination of ligustrazine in rat serum by polymer monolithic micro-extraction combined with ultra high performance liquid chromatography-tandem mass spectrometry

Sun

Peng

et al. 2021

Acta Chromatographica

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This study focused on developing an effective and environmentally friendly method to measure ligustrazine in rat serum by using polymer monolith micro-extraction (PMME) technique. A poly (methacrylic acid-ethylene glycol dimethacrylate) material was used to extract ligustrazine through hydrophobic and ion-exchange interaction. Qualitative and quantitative analysis was performed by a liquid chromatography and tandem mass spectrometry. After optimization of several PMME conditions, the developed method exhibited excellent extraction performance to the ligustrazine. Good linearity was acquired ranging from 10 to 2,000 ng mL−1, and the limit of detection of the proposed method was 0.14 ng mL−1. The recoveries measured by spiking three different concentrations in rat serum ranged from 82.6 to 95.3%, and excellent precision was found with relative standard deviations (RSDs) less than 8.3% for intra-day and 9.7% for inter-day, respectively. At last, the applicability of the method was further confirmed through continuous monitoring of ligustrazine in rat serum after dosing of ligustrazine tablets to rats.

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Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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Highly Sensitive Determination for Catecholamines Using Boronate Affinity Polymer Monolith Microextraction with In-Situ Derivatization and HPLC Fluorescence Detection

Cited by 27 publications

References 38 publications

Organic Boronate Affinity Sorbent for Capture of cis-Diol Containing Compounds Eman Alzahrani

Organic Boronate Affinity Sorbent for Capture of cis-Diol Containing Compounds Eman Alzahrani

Determination of ligustrazine in rat serum by polymer monolithic micro-extraction combined with ultra high performance liquid chromatography-tandem mass spectrometry

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

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