Highly sensitive detection of Salmonella typhi using surface aminated polycarbonate membrane enhanced-ELISA

Jain, Swati; Chattopadhyay, S.; Jackeray, Richa; Abid, Cyrine; Kohli, Guneet; Singh, Harpal

doi:10.1016/j.bios.2011.09.031

Cited by 75 publications

(43 citation statements)

References 31 publications

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“…For immobilization of BR on the polycarbonate surface, the modification should be done. So far different modifications have been done (Abenojar et al 2009;Asad Ali et al 2010;Baumann et al 2013;Carosio et al 2013;Dehaye et al 2003;Gao et al 2013;Goyal et al 2012;Heymans 1997;Iwata et al 2003;Jackeray et al 2011;Jain et al 2012;Jang et al 2014a, b;Keil et al 1998;Khan et al 2006;Kim and Masuoka 2009;Kumar et al 2003Kumar et al , 2006Kumar et al , 2012Lub et al 1988;Motaung et al 2013;Resta et al 2013;Rodríguez et al 2002;Saarikoski et al 2009;Singh et al 2007;Srivastava et al 2002;Sun et al 2003;van der Wel et al 1993;Verkuijlen et al 2014;Wang et al 2000;Zajíčková et al 2001;Zhou and Osby 2010). In the present study we try to immobilize bacteriorhodopsin on polycarbonate surface by modifying of polycarbonate functional groups in two ways.…”

Section: Introductionmentioning

confidence: 94%

Assay of bacteriorhodopsin stability on polycarbonate surface by using of FTIR-ATR: a model of disk-based bioassays

Molaeirad

Asl

Khayati

et al. 2015

J Bioenerg Biomembr

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Bacteriorhodopsin (BR) is a transmembrane protein which able to transport protons through cell membrane and thus converting solar energy to electrical energy. Up to now different strategies have been used to immobilize BR. In the present study the BR has been immobilized on polycarbonate surface with two different methods. The functional groups of polycarbonate were modified in two ways (sulfuric acid, PDAC and HNO3) and then the BR was immobilized on two different modified polycarbonate surfaces. The modified surfaces were characterized by ATR-FTIR and AFM techniques. Afterward the activity of bounded BR to two different modified polycarbonate surfaces was measured. Our results show that BR bounded to modified polycarbonate surface with HNO3 (nitrated polycarbonate) has higher activity in comparison to modified with sulfuric acid (electrostatically bounded BR). Also the activities of both types of Bounded BR after 10 days were measured. The results showed that unlike electrostatically bounded BR, bounding BR to nitrated polycarbonate keeps its activity after 10 days. In conclusion, nitrated polycarbonate surface is a suitable candidate due to immobilizing BR in order to manufacture of BioCDs.

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Section: Introductionmentioning

confidence: 94%

Assay of bacteriorhodopsin stability on polycarbonate surface by using of FTIR-ATR: a model of disk-based bioassays

Molaeirad

Asl

Khayati

et al. 2015

J Bioenerg Biomembr

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“…In the previous works, the majority of the reported ELISA and immunochromatographic strips were specific to only one or two serotypes of Salmonella , with sensitivities ranged from 10 3 to 10 5 CFU mL -1 [15,31,46]. Although the tests were very sensitive, the applications for detection of the genus of Salmonella were limited.…”

Section: Sensitivity and Cross-reactivity Of The Immunochromatographimentioning

confidence: 99%

“…Due to the specificity of the selected aptamer, aptamer-based sensors are mainly used for rapid detection of common serotypes such as S. typhimurium and S. enteritidis, rather than detection of the genus [5,13,14]. Immunoassays based on the specific antigen and antibody reaction have been widely used in Salmonella detection either with the classic enzyme-linked immunosorbent assay (ELISA) [15][16][17][18] or novel nanomaterials-based biosensors [19][20][21][22]. However, due to the drawbacks of multiple steps and high-cost of these methods there is a huge demand for a low cost, portable, rapid and stable method.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody

et al. 2016

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In this study, an innovative competitive immunochromatographic strip sensor was developed for rapid detection of Salmonella based on a genus-specific antilipopolysaccharide (LPS) monoclonal antibody (mAb) and the heterogeneous coating antigen of a LPS-bovine serum albumin conjugate. Gold nanoparticles labeled anti-LPS mAb specifically reacted with the conserved outer core of the Salmonella LPS in the sample and the color formed on the T line was negatively correlated with the number of Salmonella cells. The sensitivity of Ra mutant LPS (without O-specific chains but has the conserved outer core) was 25 ng mL -1 , which explained the detection of Salmonella at the genus level. Based on the gray values on the test line, the limit of detection of Salmonella was 10 3 colony-forming unit (CFU) for all twelve typical strains of Salmonella. The analysis of common Gram-negative and Gram-positive bacteria demonstrated that the strip assay was specific to Salmonella. A milk sample test showed that Salmonella at a low level (1-5 CFU mL -1 ) was detected without complex biochemical confirmation steps, sophisticated instruments and professional training.

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“…Existing bacteria detection methods such as culturing (Allen et al 2004), immunology assays (ELISA) (Jain et al 2012;Cho and Irudayaraj 2013) and molecular biology methods (PCR) (Opet and Levin 2013; Liu et al 2013), are limited by low sensitivity, complex operation (Wang et al 2007) or high costs (Mollasalehi and Yazdanparast 2013). The requirement for big equipment is a major bottleneck for realtime and rapid detection of bacteria.…”

Section: Introductionmentioning

confidence: 99%

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

Wang

Liu

et al. 2017

Biomed Microdevices

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An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10 m, and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria. The microfluidic chip with interdigitated microelectrodes was manufactured by microfabrication methods. The interdigital microelectrode array has sufficient contact area with a bacterial suspension to improve enrichment efficiency and detection sensitivity. Bacteria in the interdigital microelectrode area of the microfluidic chip were firstly captured and enriched by pDEP. Then, in situ impedance detection of the enriched bacteria was realized by switching test conditions. Using the self-assembly microsystem, a novel quantitative detection method was established and demonstrated to detect Escherichia coli O157:H7. Experimental results showed that the detection limits of Escherichia coli O157:H7 was 5 × 10 cfu mL, and testing time was only 6 min under the optimized detection voltage of 100 mV and frequency of 500 KHz. The method was successfully used to detect Escherichia coli O157:H7 in synthetic chicken synthetic samples.

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Highly sensitive detection of Salmonella typhi using surface aminated polycarbonate membrane enhanced-ELISA

Cited by 75 publications

References 31 publications

Assay of bacteriorhodopsin stability on polycarbonate surface by using of FTIR-ATR: a model of disk-based bioassays

Assay of bacteriorhodopsin stability on polycarbonate surface by using of FTIR-ATR: a model of disk-based bioassays

Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

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