Highly Sensitive Detection of Non-Labeled Peptides Using UV Excitation Thermal Lens Microscope/Liquid Chromatography

Hiki, Shinichiro; Tokeshi, Manabu; Kakuta, Masaya; Mawatari, Kazuma; Sato, Katsuaki; Hibara, Akihide; Shimura, Kiyohito; Uchida, Naoyuki; Kitamori, Takehiko

doi:10.2116/bunsekikagaku.56.1

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…Thus, the combination of thermal lens detection with separation techniques is signicantly important for analytical chemistry as a means for distinguishing different molecules that have absorption bands at the same wavelengths. [21][22][23][24] Previously, we considered the possibility of using DIC-TLM as a detector for extended-nano chromatography. 25 Herein, the separation and sensitive detection of nonuorescent molecules were achieved using an extended-nano uidic device for the rst time.…”

Section: Introductionmentioning

confidence: 99%

Femtoliter-scale separation and sensitive detection of nonfluorescent samples in an extended-nano fluidic device

Shimizu

Mawatari

Kitamori

2014

Analyst

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The separation and sensitive detection of nonfluorescent molecules at the femtoliter (fL) scale has been achieved for the first time in a nanofluidic channel. Smaller sample volumes and higher separation efficiencies have been significant targets for liquid chromatography for many years. However, the use of packed columns hindered further miniaturization and improvement of separation efficiency. Our group recently developed a novel chromatographic method using an open nanofluidic channel to realize attoliter sample injection and a separation efficiency of several million plates per m. However, because of the extremely small optical path length, this detection method was limited to fluorescent molecules. Herein, we describe the combination of nanofluidic chromatography with differential interference contrast thermal lens microscopy (DIC-TLM), a sensitive detection method for nonfluorescent molecules developed by our group that has the ability to detect 0.61 zmol (370 molecules) with an optical path length of 350 nm. As a result, separation of a 21 fL sample containing 250 zmol was possible at the limit of detection (LOD).

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Section: Introductionmentioning

confidence: 99%

Femtoliter-scale separation and sensitive detection of nonfluorescent samples in an extended-nano fluidic device

Shimizu

Mawatari

Kitamori

2014

Analyst

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“…Since then, TLM has been successfully applied to various integrated analytical systems on microchips for environmental analysis, clinical diagnosis, food analysis, and single cell analysis [11][12][13][14][15]. TLM has also been coupled with separation methods like electrophoresis or HPLC [16][17][18]. In addition, we reported a palm-top-sized TLM device utilizing a rod lens (SELFOC microlens) and optical fiber technologies [19,20], and we integrated practical TLMs into various microchip-based analytical systems [21,22].…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of thermal lens effect for sensitive detection on microchips

et al. 2008

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Thermal lens microscope (TLM) is a sensitive detection method for nonfluorescent molecules and widely applied to detection in a capillary or on a microchip. In this paper, we developed a flexible design tool for TLM systems to meet various applications utilizing a microspace. The TL effect was modeled, including signal processing, and calculated by combining fluidic dynamics and wave optics software. The coincidence of the calculations and experiments was investigated by measuring the effects of optical path length or focus positions of the excitation beams on TL signals which are quite difficult to calculate by a conventional method. Good agreement was shown and the applicability of the TLM design tool was verified.

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Micro and Nano Chemical System on Chip

Kitamori

2007

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference

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Highly Sensitive Detection of Non-Labeled Peptides Using UV Excitation Thermal Lens Microscope/Liquid Chromatography

Cited by 4 publications

References 11 publications

Femtoliter-scale separation and sensitive detection of nonfluorescent samples in an extended-nano fluidic device

Femtoliter-scale separation and sensitive detection of nonfluorescent samples in an extended-nano fluidic device

Numerical analysis of thermal lens effect for sensitive detection on microchips

Micro and Nano Chemical System on Chip

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