Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting

Rega, Romina; Mugnano, Martina; Giudice, Danila del; Itri, Simona; Ткаченко, В. М.; Vespini, Veronica; Coppola, Sara; Mazzon, Emanuela; Oleandro, Emilia; Ferraro, Pietro; Grilli, Simonetta

doi:10.1117/12.2564859

Cited by 1 publication

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“…We call this drop the “mother drop” and Figure 1 d shows the schematic side view, where the dashed line corresponds to the liquid–air interface. The distance between the mother drop and the target slide is approximately 800 μm, which represents the activation distance for the jetting event [ 46 ]). The LN crystal is heated locally by the absorbed radiation of the CO 2 laser in case of the laser-based configuration and by the Joule effect in case of the micro-heater-based one.…”

Section: Resultsmentioning

confidence: 99%

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Rega

Mugnano

Oleandro

et al. 2020

Sensors

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The demand for sensors capable of measuring low-abundant collagen in human fluids has highly increased in recent years. Indeed, collagen is expected to be a biomarker for chronic diseases and could monitor their progression. Here we show detection of highly diluted samples of collagen at picogram level thanks to an innovative pyro-electrohydrodynamic jet (p-jet) system. Through the intense electric fields generated by the pyroelectric effect in a ferroelectric crystal, the collagen solution was concentrated on a small area of a slide that was appropriately functionalized to bind proteins. The collagen molecules were labeled by an appropriate fluorophore to show how the number of tiny droplets influences the limit of detection of the technique. The results show that the p-jet is extremely promising for overcoming the current detection limits of collagen-based products in human fluids, performing 10 times better than the enzyme-linked immunosorbent assay (ELISA) and thus paving the way for the early diagnosis of related chronic diseases.

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Section: Resultsmentioning

confidence: 99%

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Rega

Mugnano

Oleandro

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

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Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting

Abstract: This is the pre-peer reviewed version of the article "Highly sensitive detection of low abundant molecules by pyro-electrohydrodynamic jetting" whichhas been published in the final form as Invited Paper in Proc.

Cited by 1 publication

References 36 publications

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

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