Highly sensitive detection of influenza virus in saliva by real-time PCR method using sugar chain-immobilized gold nanoparticles; application to clinical studies

Suda, Yasuo; Nagatomo, Mami; Yokoyama, Risa; Ohzono, Mami; Aoyama, Kazue; Zhang, Xu; Nakajima, Kazuhiko; Murakami, Naoki; Shinoda, Tadashi; Hirota, Toshiyuki; Yanagihara, Sae; Nishi, Junichiro

doi:10.1016/j.btre.2015.05.004

Cited by 13 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oral administration of L. acidophilus L-92 [ 3 ] has been reported to improve symptoms of atopic dermatitis [ 4 , 5 , 6 ], pollen allergy [ 7 ] and perennial allergic rhinitis [ 8 ] in humans and mice. In addition, oral intake of the strain protected against influenza infection in the mouse and human [ 9 , 10 ]. Strain L-92 has immune-regulating activities in mice, such as the regulation of cytokine production, suppression of antigen-specific IgE, induction of apoptosis of antigen-stimulated T cells and induction of regulatory T cells [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Functional role of surface layer proteins of<i> Lactobacillus acidophilus</i> L-92 in stress tolerance and binding to host cell proteins

Wakai¹,

Kano²,

Karsens

et al. 2021

Bioscience of Microbiota, Food and Health

View full text Add to dashboard Cite

Lactobacillus acidophilus surface layer proteins (SLPs) self-assemble into a monolayer that is non-covalently bound to the outer surface of the cells. There they are in direct contact with the environment, environmental stressors and gut components of the host in which the organism resides. The role of L. acidophilus SLPs is not entirely understood, although SLPs seem to be essential for bacterial growth. We constructed three L. acidophilus L-92 strains, each expressing a mutant of the most abundant SLP, SlpA. Each carried a 12-amino acid c-myc epitope substitution at a different position in the protein. A strain was also obtained that expressed the SlpA paralog SlpB from an originally silent slpB gene. All four strains behaved differently with respect to growth under various stress conditions, such as the presence of salt, ox gall or ethanol, suggesting that SlpA affects stress tolerance in L. acidophilus L-92. Also, the four mutants showed differential in vitro binding ability to human host cell proteins such as uromodulin or dendritic cell (DC)-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN). Furthermore, co-culture of murine immature DCs with a mutant strain expressing one of the recombinant SlpA proteins changed the concentrations of the cytokines IL-10 and IL-12. Our data suggest that SlpA and SlpB of L. acidophilus participate in bacterial stress tolerance and binding to uromodulin or DC-SIGN, possibly leading to effective immune-modification.

show abstract

Section: Introductionmentioning

confidence: 99%

Functional role of surface layer proteins of<i> Lactobacillus acidophilus</i> L-92 in stress tolerance and binding to host cell proteins

Wakai¹,

Kano²,

Karsens

et al. 2021

Bioscience of Microbiota, Food and Health

View full text Add to dashboard Cite

show abstract

“…25,26 However, influenza A and B were detected in 20 to 60% of asymptomatic individuals. 27 Taken together, these studies suggest that (a) the oral viral community is acquired through a non-random process of microbial assembly that is partly dictated by individual genotype (b) viral communities are temporally stable once acquired and (c) exogenous viruses are present in saliva during acute phase infection, but most do not persist following resolution of disease.…”

Section: The Oral Cavity As a Reservoir For Viruses In Health And Dismentioning

confidence: 92%

Demystifying the mist: Sources of microbial bioload in dental aerosols

Kumar

Subramanian

2020

Journal of Periodontology

View full text Add to dashboard Cite

The risk of transmitting airborne pathogens is an important consideration in dentistry and has acquired special significance in the context of recent respiratory disease epidemics. The purpose of this review, therefore, is to examine (1) what is currently known regarding the physics of aerosol creation, (2) the types of environmental contaminants generated by dental procedures, (3) the nature, quantity, and sources of microbiota in these contaminants and (4) the risk of disease transmission from patients to dental healthcare workers. Most dental procedures that use ultrasonics, handpieces, air‐water syringes, and lasers generate sprays, a fraction of which are aerosolized. The vast heterogeneity in the types of airborne samples collected (spatter, settled aerosol, or harvested air), the presence and type of at‐source aerosol reduction methods (high‐volume evacuators, low volume suction, or none), the methods of microbial sampling (petri dishes with solid media, filter paper discs, air harvesters, and liquid transport media) and assessment of microbial bioload (growth conditions, time of growth, specificity of microbial characterization) are barriers to drawing robust conclusions. For example, although several studies have reported the presence of microorganisms in aerosols generated by ultrasonic scalers and high‐speed turbines, the specific types of organisms or their source is not as well studied. This paucity of data does not allow for definitive conclusions to be drawn regarding saliva as a major source of airborne microorganisms during aerosol generating dental procedures. Well‐controlled, large‐scale, multi center studies using atraumatic air harvesters, open‐ended methods for microbial characterization and integrated data modeling are urgently needed to characterize the microbial constituents of aerosols created during dental procedures and to estimate time and extent of spread of these infectious agents.

show abstract

“…Even though the capability of the Boom method has been improved by several companies including Qiagen, it still takes time and it also extracts and purifies free RNA of SARS-CoV-2 in specimens that have no infectious activity due to the loss of spike protein or envelope in the matured viral structure. We applied our nano-biotechnology, SGNP method [10][11][12][13][14], in which sugar chain-immobilized magnetized gold nanoparticles and micro-meter sized magnetic particles were used to collect and purify virions that have matured spike protein and viral particles. Then, by adding detergent solution to the collected mixture, viral RNAs were extracted from the purified virion, followed by applying the solution to qRT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients’ Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients

Kajiya¹,

Sawayama

Arima

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

Background: The transmissible capacity and toxicity of SARS-CoV-2 variants are continually changing. We report here the follow-up study of hospitalized COVID-19 patients from 2020 to 2022. It is known that the PCR diagnosis for hospitalized patients sometimes causes confusion because of the incompatibility between their diagnosis and symptoms. We applied our sugar chain-immobilized gold-nanoparticles for the extraction and partial purification of RNA from specimens for quantitative RT-PCR assay and evaluated whether the results correlate with patients’ symptoms. Methods and Results: Saliva specimens were taken from hospitalized patients with mild or moderate symptoms every early morning. At the time of RT-PCR diagnosis, two methods for the extraction and partial purification of RNA from the specimen were performed: a commonly used Boom (Qiagen) method and our original sugar chain-immobilized gold nanoparticle (SGNP) method. For symptoms, body temperature and oxygen saturation (SpO2) of patients were monitored every 4 h. Conclusions: It was clear that patients infected with the Delta variant needed more time to recover than those with the Omicron variant, and that the SGNP method showed more realistic correlation with the symptoms of patients compared with the common Qiagen method.

show abstract

Highly sensitive detection of influenza virus in saliva by real-time PCR method using sugar chain-immobilized gold nanoparticles; application to clinical studies

Abstract: Graphical abstract

Cited by 13 publications

References 23 publications

Functional role of surface layer proteins of<i> Lactobacillus acidophilus</i> L-92 in stress tolerance and binding to host cell proteins

Functional role of surface layer proteins of<i> Lactobacillus acidophilus</i> L-92 in stress tolerance and binding to host cell proteins

Demystifying the mist: Sources of microbial bioload in dental aerosols

Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients’ Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients

Contact Info

Product

Resources

About