Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor

Gnedenko, O. V.; Mezentsev, Yu. V.; Molnar, A. A.; Lisitsa, Andrey; Ivanov, A. S.; Archakov, Alexander I.

doi:10.1016/j.aca.2012.10.053

Cited by 100 publications

(53 citation statements)

References 27 publications

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“…The use of nanomaterials in signal enhancement for the detection of an analyte in SPR has been intensely studied. Most commonly applied nanomaterials include latex nanoparticles [38], metallic nanoparticles e.g., gold and silver nanoparticles [39,40], magnetic nanoparticles [41,42], carbon-based nanostructures e.g., graphene [40,43] and liposome nanoparticles [41,44]. However, the use of the above nanoparticles in the enhancement of SPR-based assays of biological materials are predominantly for protein and small molecules.…”

Section: Sandwich Assay With Signal Amplification Using Aunpsmentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Section: Sandwich Assay With Signal Amplification Using Aunpsmentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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“…A first monoclonal anti-cMB antibody was covalently immobilized on the sensor surface and then AuNPs were covalently immobilized to the second monoclonal anti-cMb antibody. The limit of detection of cMb in a human blood serum sample was found to be as low as 10 pM with an intra-assay coefficient variation of less than 3% [40]. …”

Section: Nanomodified Spr-abbs Applications For In Vitro Diagnosticsmentioning

confidence: 99%

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Antiochia

Bollella

Favero

et al. 2016

International Journal of Analytical Chemistry

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In the last decades, in vitro diagnostic devices (IVDDs) became a very important tool in medicine for an early and correct diagnosis, a proper screening of targeted population, and also assessing the efficiency of a specific therapy. In this review, the most recent developments regarding different configurations of surface plasmon resonance affinity biosensors modified by using several nanostructured materials for in vitro diagnostics are critically discussed. Both assembly and performances of the IVDDs tested in biological samples are reported and compared.

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“…A three-electrode system was employed with gold as the working electrode, silver/silver chloride as the reference electrode, and platinum as the counter electrode. CV and DPV were carried out in a degassed 10 …”

Section: Electrochemical Analysismentioning

confidence: 99%

“…[3][4][5] Optical biosensors, including a sandwich immunoassay with secondary labeled antibodies, enzyme-linked immunosorbent assay (ELISA), fluorescence, and surface plasma resonance (SPR), have been used for the detection of cardiac markers, such as troponin I, myoglobin, c-reactive protein (CRP), and myeloperoxidase (MPO). [6][7][8][9][10] Driven by a clinical need for pointof-care diagnostics, next-generation biosensors with improved sensitivity and specificity have attracted increasing attention in recent years, leading to the development of peptide-based sensing approaches. 11 Peptides derived from phage display are attractive alternatives to biosensor probes.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction

et al. 2015

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Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor

Cited by 100 publications

References 27 publications

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction

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