Highly sensitive detection of cardiac troponin I in human serum using gold nanoparticle-based enhanced sandwich immunoassay

Kim, Wan‐Joong; Cho, Hyo Young; Kim, Bong Kyu; Huh, Chul; Chung, Kwang Hyo; Ahn, Chang–Geun; Kim, Young Jun; Kim, Ansoon

doi:10.1016/j.snb.2015.06.128

Cited by 32 publications

(14 citation statements)

References 26 publications

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“…AuNP•antiCRP-HRPs were prepared using AuNPs of different diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm and 50 nm) based on procedures previously described [ 22 ]. Briefly, using AuNP of 10 nm as an example, 1.0 mL of the AuNP solutions (9.46 nM) was mixed with 20 μL of potassium carbonate (K 2 CO 3 , 40 mM) by adjusting pH at 9.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Kim

Cho

Jeong

et al. 2017

Sensors

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Using gold nanoparticles (AuNPs) on “capillary enzyme-linked immunosorbent assay (ELISA)”, we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of AuNPs, AuNPs of varying diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm, and 50 nm) conjugated with Horseradish Peroxidase (HRP)-labeled anti-C reactive protein (antiCRP) (AuNP•antiCRP-HRP) were used for well-plate ELISA. AuNP of 10 nm produced the largest optical density, enabling detection of 0.1 ng/mL of CRP with only 30 s of incubation, in contrast to 10 ng/mL for the ELISA run in the absence of AuNP. Then, AuNP of 10 nm conjugated with antiCRP-HRP (AuNP•antiCRP-HRP) was used for “capillary ELISA” to detect as low as 0.1 ng/mL of CRP. Also, kinetic study on both 96-well plates and in a capillary tube using antiCRP-HRP or AuNP•antiCRP-HRP showed a synergistic effect between AuNP and the capillary system, in which the fastest assay was observed from the “AuNP capillary ELISA”, with its maximum absorbance reaching 2.5 min, while the slowest was the typical well-plate ELISA with its maximum absorbance reaching in 13.5 min.

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Section: Methodsmentioning

confidence: 99%

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Kim

Cho

Jeong

et al. 2017

Sensors

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“…65,66 For example, troponin I antigen, anti-Listeria monocytogenes monoclonal antibodies and anti-HIV-1 p24 monoclonal antibody functionalized Au NPs were used for the detection of cardiac troponin I, Listeria monocytogenes, HIV-1 p24 antigen, with LODs of 15 pg mL −1 , 3.5 CFU mL −1 and 1.49 pg mL −1 , respectively. [67][68][69] On the basis of aptamerprotein interactions, Chang et al achieved a sensitive detection of platelet derived growth factors (PDGFs) using aptamer-Au NP probes. 70 Combining aptamer modified Au NPs (13 nm) and fibrinogen adsorbed Au NPs (56 nm), they realized PDGF detection with improved sensitivity at the picomolar level.…”

Section: Colorimetric Assaysmentioning

confidence: 99%

Gold nanoparticles as sensitive optical probes

Yuan

Chang

et al. 2016

Analyst

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Gold nanoparticles (Au NPs) have become one of the most popular materials for sensing of analytes of interest in the last decade, mainly because of their ease in preparation and conjugation, stability, biocompatibility, and size-dependent optical properties. We have witnessed many sensitive and selective Au NP based optical systems for the quantitation of metal ions, anions, proteins, and DNA, based on analyte induced changes in their absorption, fluorescence, and scattering. In this tutorial review, we briefly discuss wet chemical approaches for the preparation of Au NPs. Sensing mechanisms and strategies of Au NP based optical systems are provided to show basic concepts in designing sensitive and selective sensing systems. Strategies for signal amplification applied in Au NP based systems are emphasized for the analysis of trace amounts of analytes in real samples. Many excellent Au NP based optical sensing systems are discussed to highlight their practicality for the analysis of complicated biological and environmental samples. The tutorial review ends with the discussion of the challenges and future trends of Au NP based optical sensing systems.

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“…Labelling requires an additional preparation step and, depending on the selected label, suitable storage conditions should be determined. Kim et al have proposed an enhanced immunogold assay using a silver staining technique for signal amplification [130]. The immunoassay was conducted in a direct and enhanced manner, the latter involving binding of the antigen to AuNP-labelled detection Abs in a sample solution prior to being captured.…”

Section: Impact Of Labelsmentioning

confidence: 99%

“…Figure 3 and Figure 4: [37,38,48,49,50,57,58,61,65,66,68,69,70,71,74,84,85,86,87,88,89,93,94,95,96,97,104,105,111,112,115,116,117,120,121,122,123,125,126,127,128,130,132,133,134,136,138,144,145,148,150,151,159,161,174,175,176,184,185,186,187,188,189,190,…”

Section: Reprinting Figuresmentioning

confidence: 99%

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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Cardiac biomarkers are frequently measured to provide guidance on the well-being of a patient in relation to cardiac health with many assays having been developed and widely utilised in clinical assessment. Effectively treating and managing cardiovascular disease (CVD) relies on swiftly responding to signs of cardiac symptoms, thus providing a basis for enhanced patient management and an overall better health outcome. Ultra-sensitive cardiac biomarker detection techniques play a pivotal role in improving the diagnostic capacity of an assay and thus enabling a better-informed decision. However, currently, the typical approach taken within healthcare depends on centralised laboratories performing analysis of cardiac biomarkers, thus restricting the roll-out of rapid diagnostics. Point-of-care testing (POCT) involves conducting the diagnostic test in the presence of the patient, with a short turnaround time, requiring small sample volumes without compromising the sensitivity of the assay. This technology is ideal for combatting CVD, thus the formulation of ultra-sensitive assays and the design of biosensors will be critically evaluated, focusing on the feasibility of these techniques for point-of-care (POC) integration. Moreover, there are several key factors, which in combination, contribute to the development of ultra-sensitive techniques, namely the incorporation of nanomaterials for sensitivity enhancement and manipulation of labelling methods. This review will explore the latest developments in cardiac biomarker detection, primarily focusing on the detection of cardiac troponin I (cTnI). Highly sensitive detection of cTnI is of paramount importance regarding the rapid rule-in/rule-out of acute myocardial infarction (AMI). Thus the challenges encountered during cTnI measurements are outlined in detail to assist in demonstrating the drawbacks of current commercial assays and the obstructions to standardisation. Furthermore, the added benefits of introducing multi-biomarker panels are reviewed, several key biomarkers are evaluated and the analytical benefits provided by multimarkers-based methods are highlighted.

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Highly sensitive detection of cardiac troponin I in human serum using gold nanoparticle-based enhanced sandwich immunoassay

Cited by 32 publications

References 26 publications

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Gold nanoparticles as sensitive optical probes

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

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