Highly Sensitive Detection of Carbaryl Pesticides Using Potentiometric Biosensor with Nanocomposite Ag/r-Graphene Oxide/Chitosan Immobilized Acetylcholinesterase Enzyme

Abstract

Novel, sensitive, selective, efficient and portable electrochemical biosensors are needed to detect residual contaminants of the pesticide 1-naphthyl methylcarbamate (carbaryl) in the environment, food, and essential biological fluids. In this work, a study of nanocomposite-based Ag reduced graphene oxide (rGO) and chitosan (CS) that optimise surface conditions for immobilisation of acetylcholinesterase (AChE) enzyme to improve the performance of catalytic biosensors is examined. The Ag/rGO/CS nanocomposite membrane was used to determine carbaryl pesticide using a potentiometer transducer. The AChE enzyme-based biosensor exhibits a good affinity for acetylthiocholine chloride (ATCl). It can catalyse the hydrolysis of ATCl with a potential value of 197.06 mV, which is then oxidised to produce a detectable and rapid response. Under optimal conditions, the biosensor detected carbaryl pesticide at concentrations in the linear range of 1.0 × 10−8 to 1.0 μg mL−1 with a limit of detection (LoD) of 1.0 × 10−9 μg mL−1. The developed biosensor exhibits a wide working concentration range, detection at low concentrations, high sensitivity, acceptable stability, reproducibility and simple fabrication, thus providing a promising tool for pesticide residue analysis.

Manufacture and characterization of indicator electrodes from PPy + H2SO4 and PPy + Sulfonic acid as a urea sensor using urease enzyme immobilization technique in PVA

“…The procedure of this study followed (a) Analysis of transmittance, edx, xrd [27], edx, xrd, linear curve, sensitivity and detection range [28], transmittance, concentration, sensitivity [29][30][31]; (b) The biosensor system [20], namely (1) selectivity, (2) sensitivity, (3) linearity response, namely the concentration range of the target analyte to be measured, (4) reproducibility of signal response, samples having a different concentration. the same analyzed several times should give the same response, (5) fast response time and recovery time for reusability of the biosensor system, (6) stability and operating life; (c) The [7] immobilization technique was developed based on three important mechanisms, namely (1) physical adsorption, (2) covalent immobilization, (3) streptavidin-biotin immobilization.…”

Development of Fluorescent Co (II)-Integrated Carbon Dots and Their Application as a Off–On Mesotrione Detection Sensor

“…A thorough comparison of the results obtained in the current study with those of the reported literature being summarized in Table supports the superiority of the sensing probe designed in the current study. The parameters selected for comparison are percentage recovery, determination coefficient ( R 2 ), limit of detection, limit of quantification, % relative standard deviation, and linear range. ,− …”

“…The parameters selected for comparison are percentage recovery, determination coefficient ( R 2 ), limit of detection, limit of quantification, % relative standard deviation, and linear range. 49 , 53 − 54 55 56 57 58 59 60 61 …”

Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice

“…The AChE/CS-GO/GO/CNFs layers demonstrated excellent surface conductivity due to the presence of the GO, which has a large surface area, high electrical conductivity, a high electron transfer rate, and the ability to immobilize various molecules. 46 AChE was loaded deeply into the layers with the aid of a chitosan adhesive agent, 47 enhancing the sensitivity and stability of the modified electrode. Then, in order to confirm the sensitivity and viability of our biosensor for the detection of chlorpyrifos and other metal ions in actual applications, we tested it on commercial drinking water and juice samples.…”