Highly sensitive detection of aflatoxin B1 byCRISPR/Cas12a-assisted single nanoparticle counting

Single Particle Analysis-Enhanced DNA Walking Machine for Sensitive miRNA Detection

“…37 SP-ICPMS offers superior sensitivity and can effectively distinguish between background noise and detection signals. 38,39 In recent years, many groups have been dedicated to developing reliable SP-ICPMS bioanalytical strategies. 40−43 For example, Zhang et al combined SP-ICPMS with CRISPR/ Cas12a and proposed a SP-ICPMS-based CRISPR/Cas12a bioanalytical counting strategy to achieve sensitive detection.…”

“…40−43 For example, Zhang et al combined SP-ICPMS with CRISPR/ Cas12a and proposed a SP-ICPMS-based CRISPR/Cas12a bioanalytical counting strategy to achieve sensitive detection. 38 Huang et al provided a differential assay strategy, which calculated the difference in the number of unbound nanoparticle tags on the surface of the magnetic beads (MBs) before and after the immune response by SP-ICPMS, to accurately evaluate the biomarkers of gastric cancer without washing. 44 Prostate cancer (PCa) stands as one of the leading causes of cancer-related mortality among males.…”

“…It not only preserves the advantages of ICPMS but also rapidly provides information such as size, size distribution, nanoparticle concentration, and agglomeration of metal nanoparticles. , Hence, SP-ICPMS can readily reveal the differentiation between individual nanoparticles . In addition, owing to the large amount of metal atoms in a nanoparticle and the concentrating effect of single nanoparticle detection, SP-ICPMS offers superior sensitivity and can effectively distinguish between background noise and detection signals. , In recent years, many groups have been dedicated to developing reliable SP-ICPMS bioanalytical strategies. − For example, Zhang et al combined SP-ICPMS with CRISPR/Cas12a and proposed a SP-ICPMS-based CRISPR/Cas12a bioanalytical counting strategy to achieve sensitive detection . Huang et al provided a differential assay strategy, which calculated the difference in the number of unbound nanoparticle tags on the surface of the magnetic beads (MBs) before and after the immune response by SP-ICPMS, to accurately evaluate the biomarkers of gastric cancer without washing …”