2018
DOI: 10.3389/fmicb.2018.00179
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Highly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles

Abstract: A simple, sensitive and selective colorimetric biosensor for the detection of Staphylococcal enterotoxin B (SEB) was developed using SEB-binding aptamer (SEB2) as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric probes. The assay is based on color change from red to purple due to conformational change of aptamer in the presence of SEB, and the phenomenon of salt-induced AuNPs aggregation which could be monitored by naked eye or UV–vis spectrometer. Results showed that the AuNPs can… Show more

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Cited by 73 publications
(28 citation statements)
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“…Absorbance values of 520 nm and 630 nm represent the dispersion and aggregation phenomenon of AuNPs. Therefore, the absorbance peak ratio of A 630/520 nm was used to determine the aggregation 26 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Absorbance values of 520 nm and 630 nm represent the dispersion and aggregation phenomenon of AuNPs. Therefore, the absorbance peak ratio of A 630/520 nm was used to determine the aggregation 26 .…”
Section: Resultsmentioning
confidence: 99%
“…The characterization of apt/AuNPs nanocomplex (+/− Afl M1) was done by UV-Vis spectrophotometer, DLS (Dynamic Light Scattering) for hydrodynamic diameter and zeta potential measurements and TEM (Transmission Electron Microscope) for morphological and size measurements. The Afl M1 apt/AuNPs complex was formed via simple physisorption of specific aptamers onto the surface of AuNPs 26 . Afl M1 was spiked in water and milk, followed by direct application on the µPAD and change in colour was observed immediately after addition.…”
mentioning
confidence: 99%
“…On the other hand, if the solution presents aptamer targets, the structure of the aptamer will be changed, and a new structure will be formed with the target. Therefore, gold nanoparticles lose the aptamer on their surface when induced with NaCl, resulting in nanoparticle aggregation and a visible color change of the solution from red to purple [ 24 25 26 ]. The 1F aptamer was the best aptamer among the candidates and showed specificity against the PRSSV by a colorimetric assay involving 3 different porcine viruses.…”
Section: Discussionmentioning
confidence: 99%
“…• Based on the dimension: The nanobiosensor products can be classified into three categories based on the dimensions which are pushed to the nanometer scale: a. [63], [64] Optical absorption-based nanobiosensors [65] Fluorescent nanobiosensors [66], [67] Light scattering nanobiosensors [68], [69] Surface plasmon resonance (SPR) based nanobiosensors [70], [71] Surface-enhanced Raman scattering (SERS) nanobiosensors [72], [73] Inductively coupled plasma mass spectroscopy (ICP-MS) nanobiosensors [74], [75] • Ultrahigh sensitivity: Use of nanotechnology has made biosensors more adept in terms of detection capability and sensitivity.…”
Section: ) Classifications Of Nanobiosensorsmentioning
confidence: 99%