Highly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles

Mondal, Bhairab; Ramlal, Shylaja; Lavu, Padma Sudha Rani; Bhavanashri, N; Kingston, Joseph J.

doi:10.3389/fmicb.2018.00179

Cited by 66 publications

(23 citation statements)

References 22 publications

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“…Absorbance values of 520 nm and 630 nm represent the dispersion and aggregation phenomenon of AuNPs. Therefore, the absorbance peak ratio of A 630/520 nm was used to determine the aggregation 26 .…”

Section: Resultsmentioning

confidence: 99%

“…The characterization of apt/AuNPs nanocomplex (+/− Afl M1) was done by UV-Vis spectrophotometer, DLS (Dynamic Light Scattering) for hydrodynamic diameter and zeta potential measurements and TEM (Transmission Electron Microscope) for morphological and size measurements. The Afl M1 apt/AuNPs complex was formed via simple physisorption of specific aptamers onto the surface of AuNPs 26 . Afl M1 was spiked in water and milk, followed by direct application on the µPAD and change in colour was observed immediately after addition.…”

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confidence: 99%

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Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Kasoju

Shahdeo

Khan

et al. 2020

Sci Rep

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This study describes the colorimetric detection of aflatoxin M1 (Afl M1) in milk samples using a microfluidic paper-based analytical device (µPAD). Fabrication of µPADs was done using a simple and quick approach. Each μPAD contained a detection zone and a sample zone interconnected by microchannels. The colorimetric assay was developed using unmodified AuNPs as a probe and 21-mer aptamer as a recognition molecule. The free aptamers were adsorbed onto the surface of AuNPs in absence of Afl M1, even at high salt concentrations. The salt induced aggregation of specific aptamers occurred in presence of Afl M1. Under optimum conditions, the analytical linear range was found to be 1 µM to 1 pM with limit of detection 3 pM and 10 nM in standard buffer and spiked milk samples respectively. The proposed aptamer based colorimetric assay was repeatable, quick, selective, and can be used for on-site detection of other toxins in milk and meat samples. Mycotoxins are secondary metabolites produced by filamentous fungi belonging to the genera Aspergillus and Penicillium 1. Mycotoxins are also found in animal derived foods such as milk due to intake of contaminated feed 2,3. Considering agricultural and economical aspects and possible implications on public health, the most relevant mycotoxins are aflatoxins, ochratoxins, fuminosins, T-2 toxin, and zearalenone (ZEA) 4. Aflatoxins pose a huge economic burden as they cause around 25% or more loss of the world's food crops every year 5. Afl M1 (4-hydroxy aflatoxin B1) and M2 (4-dihydroxy aflatoxin B1) have well proven carcinogenic and mutagenic potentiality and pose severe health consequences on milk consumers, including the risk of cancer and stunted growth in children below the age of 5 years 6. When B1 is ingested by a cow, it is secreted as hydroxylated metabolite aflatoxin M1 (Afl M1) in the urine and milk of the cow 7. Consumption of food containing aflatoxin concentrations of one milligram/kilogram or higher has been suspected to cause aflatoxicosis 8 , the prognosis of which consists of acute liver failure, jaundice, lethargy, and nausea, eventually leading to death within 1 to 2 weeks, based on past reports 9. Thus there is a need to develop rapid low cost technology based highly specific methods for detection of aflatoxins to improve surveillance and control in rural areas. A plethora of analytical techniques are available for aflatoxin M1 detection, ranging from chromatography and HPLC-MS used for regulatory control in official laboratories to rapid test kits for grain silos and farmers, especially for surveys when outbreaks occur 10,11. Conventional techniques for the detection of aflatoxins include gas chromatography (GC), fluorescence or UV based detection, thin layer chromatography (TLC) and enzyme linked immunosorbent assay (ELISA) 6-12. These techniques are routinely used and yield reliable results, however they are expensive, time consuming, require large scale instrumentation and large amounts of hazardous chemical reagents. Advancement in the development of bios...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Kasoju

Shahdeo

Khan

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…On the other hand, if the solution presents aptamer targets, the structure of the aptamer will be changed, and a new structure will be formed with the target. Therefore, gold nanoparticles lose the aptamer on their surface when induced with NaCl, resulting in nanoparticle aggregation and a visible color change of the solution from red to purple [ 24 25 26 ]. The 1F aptamer was the best aptamer among the candidates and showed specificity against the PRSSV by a colorimetric assay involving 3 different porcine viruses.…”

Section: Discussionmentioning

confidence: 99%

Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus

et al. 2020

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Background: Recently, the pork industry of Thailand faced an epidemic of highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), which spread throughout Southeast Asia, including the Lao People's Democratic Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on a farm is essential. Objectives: To develop the new aptamer as a biosensor for detection PRRSV which are rapid and on-site screening of infected pig. Methods: New aptamers against PRSSV were identified using the combined techniques of capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz crystal microbalance (QCM). Results: Thirty-six candidate aptamers of the PRRSV were identified from the systematic evolution of ligands by exponential enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, only the 1F aptamer could bind specifically to the PRSSV when presented with the classical swine fever virus and a pseudo rabies virus. The QCM was used to confirm the specificity and sensitivity of the 1F aptamer with a detection limit of 1.87 × 10 10 particles. Conclusions: SELEX screening of the aptamer equipped with capillary electrophoresis potentially revealed promising candidates for detecting the PRRSV. The 1F aptamer exhibited the highest specificity and selectivity against the PRRSV. These findings suggest that 1F is a promising aptamer for further developing a novel PRRSV rapid detection kit.

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“…• Based on the dimension: The nanobiosensor products can be classified into three categories based on the dimensions which are pushed to the nanometer scale: a. [63], [64] Optical absorption-based nanobiosensors [65] Fluorescent nanobiosensors [66], [67] Light scattering nanobiosensors [68], [69] Surface plasmon resonance (SPR) based nanobiosensors [70], [71] Surface-enhanced Raman scattering (SERS) nanobiosensors [72], [73] Inductively coupled plasma mass spectroscopy (ICP-MS) nanobiosensors [74], [75] • Ultrahigh sensitivity: Use of nanotechnology has made biosensors more adept in terms of detection capability and sensitivity.…”

Section: ) Classifications Of Nanobiosensorsmentioning

confidence: 99%

Advancing Modern Healthcare With Nanotechnology, Nanobiosensors, and Internet of Nano Things: Taxonomies, Applications, Architecture, and Challenges

Pramanik

Solanki

Debnath³

et al. 2020

IEEE Access

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Healthcare sector is probably the most benefited from the applications of nanotechnology. The nanotechnology, in the forms of nanomedicine, nanoimplants, nanobiosensors along with the internet of nano things (IoNT), has the potential to bring a revolutionizing advancement in the field of medicine and healthcare services. The primary aim of this paper is to explore the clinical and medical possibilities of these different implementations of nanotechnology. This paper provides a comprehensive overview of nanotechnology, biosensors, nanobiosensors, and IoNT. Furthermore, multilevel taxonomies of nanotechnology, nanoparticles, biosensors, nanobiosensors, and nanozymes are presented. The potential medical and clinical applications of these technologies are discussed in details with several examples. This paper specifically focuses on IoNT and its role in healthcare. In addition to describing a general architecture of IoNT for healthcare, the communication architecture of the IoNT is also explained. The challenges in the successful realization of IoNT are also discussed critically, along with a special discussion on internet of bio-nano things (IoBNT) and its potential in making IoNT more compatible to human body.

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Highly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles

Cited by 66 publications

References 22 publications

Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus

Advancing Modern Healthcare With Nanotechnology, Nanobiosensors, and Internet of Nano Things: Taxonomies, Applications, Architecture, and Challenges

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