Highly sensitive capacitance-based nitrite sensing using polydopamine/AuNPs-modified screen-printed carbon electrode

Algethami, Faisal K.; Rabti, Amal; Abdulkhair, Babiker Y.; Aoun, Sami Ben; Raouafi, Noureddine

doi:10.1039/d3ra03898j

Cited by 4 publications

(2 citation statements)

References 66 publications

(88 reference statements)

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“…Electrochemical methods have gained widespread attention due to their simplicity, cost-effectiveness, ease of operation, high sensitivity, and rapid sensing capabilities. 5,14–18 Indeed, they allow the preparation of sensing devices that fit the World Health Organization “ASSURED: Affordable, Sensitive, Selective, User-friendly, Equipment-free and Deliverable to end-users” criteria. 19,20 For instance, Gökçe et al 21 developed an aptamer-based electrochemical sensor for OTA detection, demonstrating practical application in samples using pencil graphite electrodes.…”

Section: Introductionmentioning

confidence: 99%

Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes

Argoubi,

Algethami,

Raouafi

2024

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes

Argoubi,

Algethami,

Raouafi

2024

RSC Adv.

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“…Biosensors are currently sought for uses in different fields of applications such diagnostics, food safety and environmental monitoring because of their cost-effectiveness and amiability [10][11][12][13][14][15]. Peptides have recently been re-examined and their uses as recognition element have been investigated [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

In-silico selection of peptides for the recognition of imidacloprid

Aldulaijan

2023

PLoS ONE

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The sensitive detection of pesticides using low-cost receptors designed from peptides can widen their uses in the environmental surveillance for emerging pollutants. In-silico selection of peptides can help accelerate the design of receptor sequence banks for a given target of interest. In this work, we started from Lymnaea stagnalis acetylcholine-binding protein Q55R mutant receptor-imidacloprid complex, available in the PDB databank, to select three primary short peptides (YSP09, DMR12, WQW13 respectively having 9, 12 and 13 amino acids (AA) in length) from the pesticide interacting zones with the A, B and C chains of the nicotinic receptor. Using molecular docking and molecular dynamics (MD) simulations, we showed that the three peptides can form complexes with the target imidacloprid, having energies close to that obtained from a reference RNR12 peptide. Combination of these peptides allowed preparing a new set of longer peptides (YSM21, PSM22, PSW31 and WQA34) that have higher stability and affinity as shown by the MM-PBSA calculations. In particular, the WQA34 peptide displayed an average binding free energy of –6.44±0.27 kcal/mol, which is three times higher than that of the reference RNR12 peptide (–2.29±0.25 kcal/mol) and formed a stable complex with imidacloprid. Furthermore, the dissociation constants (Kd), calculated from the binding free energy, showed that WQA32 (40 μM) has three orders of magnitude lower Kd than the reference RNR12 peptide (3.4 × 104 μM). Docking and RMSD scores showed that the WQA34 peptide is potentially selective to the target imidacloprid with respect to acetamiprid and clothianidin. Therefore, this peptide can be used in wet-lab experiments to prepare a biosensor to selectively detect imidacloprid.

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Gold-modified laser-scribed lettuce-like graphene electrodes for ultrasensitive detection of bioactive molecules

Raouafi,

Diedhiou,

Almarri

et al. 2024

emergent mater.

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Highly sensitive capacitance-based nitrite sensing using polydopamine/AuNPs-modified screen-printed carbon electrode

Abstract: Polydopamine/AuNPs/SPCE electrodes are useful for the detection of nitrite ions in processed meat samples using redox capacitance spectroscopy.

Cited by 4 publications

References 66 publications

Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes

Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes

In-silico selection of peptides for the recognition of imidacloprid

Gold-modified laser-scribed lettuce-like graphene electrodes for ultrasensitive detection of bioactive molecules

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