Highly Sensitive and Selective Sensor Chips with Graphene-Oxide Linking Layer

Abstract

The development of sensing interfaces can significantly improve the performance of biological sensors. Graphene oxide provides a remarkable immobilization platform for surface plasmon resonance (SPR) biosensors due to its excellent optical and biochemical properties. Here, we describe a novel sensor chip for SPR biosensors based on graphene-oxide linking layers. The biosensing assay model was based on a graphene oxide film containing streptavidin. The proposed sensor chip has three times higher sensitivity than the carboxymethylated dextran surface of a commercial sensor chip. Moreover, the demonstrated sensor chips are bioselective with more than 25 times reduced binding for nonspecific interaction and can be used multiple times. We consider the results presented here of importance for any future applications of highly sensitive SPR biosensing.

Graphene Oxide as an Optical Biosensing Platform: A Progress Report

“…[91] Chen and co-workers explored an optical fiber (particularly, dual-peak long-period grating) decorated with GO and antibodies targeting anti-IgG, see Figure 5A. A 2.9-fold enhancement was discussed in this biosensing approach studied by Stebunov et al, [98] see Figure 5B. [92] In fact, the GO-coated fiber approach showed a limit of detection tenfold lower than that of the noncoated fiber.…”

“…[90] Optical fibers are a particular example of optical waveguides. [98] In fact, GO-coated SPR substrates have been reported to outperform the bioanalytical sensitivity of commercially available biochips containing a 3D linker. The authors reported that the studied biodetection platform surpasses its counterpart without GO due to the improved chemical bonding and physical adsorption of the biorecognition enabled by GO.…”

“…[20,[111][112][113] iv) A charge transferring phenomenon that improves SERS performance. [98] Copyright 2015 American Chemical Society. [115] Moreover, GO leads to generation of flexible membrane-like SERS substrates than can be utilized as pre-concentration platforms for highly sensitive analysis of large volumes.…”

“…[97] Moreover, spectroscopic ellipsometry revealed that GO-coated SPR substrates displayed much lower optical absorption in the visible range than its counterpart coated with pristine graphene. [98] In fact, GO-coated SPR substrates have been reported to outperform the bioanalytical sensitivity of commercially available biochips containing a 3D linker. A 2.9-fold enhancement was discussed in this biosensing approach studied by Stebunov et al, [98] see Figure 5B.…”

Graphene based biosensors—Accelerating medical diagnostics to new-dimensions

“…Numerous graphene-based biosensors have shown remarkable performance from diagnosis of disease to their treatment and have begun to make their way to the market place. For instance, Stebunov and co-workers 173 have demonstrated the first SPR biosensor chip by using a linked layer for biomolecule immobilization, comprising of a thin film of graphene or GO, for optical biosensing applications. The proposed biosensor chip showed good sensitivity with high binding sites and less nonspecific binding.…”