Highly sensitive and selective electrochemical detection of lipocalin 2 by NiO nanoparticles/perovskite CeCuOx based immunosensor to diagnose renal failure

Chakraborty, Titisha; Das, Madhusweta; Lin, Chan Yu; Lei, Kin Fong; Kao, Chiang

doi:10.1016/j.aca.2022.339754

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…blank. Although others have reported a similar performance using gold electrodes in a standard three-electrode cell or improved sensitivity using a combination of carbon electrodes and nanomaterials, as presented in Table 7, this work was the first in which such a detection was demonstrated using a commercially available gold SPE for the electrochemical immunosensor in bodily fluids [47,[58][59][60][61][62][63][64][65][66]. The logistic regression equation is given as 𝐼 = 𝐴 + 𝐵 × ln (𝑥), where A is 228 and B is 202, with an r 2 = 0.9822.…”

Section: Sensing Platform Evaluationmentioning

confidence: 70%

“…It shows a linear relationship between the steady-state current and the logarithmic values of the antigen concentration in the range from 3.5 ng/mL to 80 ng/mL with a theoretical detection limit (LOD) of 1.0 ng/mL, estimated as the mean blank level + 3 × standard deviation of the blank. Although others have reported a similar performance using gold electrodes in a standard three-electrode cell or improved sensitivity using a combination of carbon electrodes and nanomaterials, as presented in Table 7, this work was the first in which such a detection was demonstrated using a commercially available gold SPE for the electrochemical immunosensor in bodily fluids [47,[58][59][60][61][62][63][64][65][66].…”

Section: Sensing Platform Evaluationmentioning

confidence: 71%

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Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

et al. 2023

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State-of-the-art clinical detection methods typically involve standard immunoassay methods, requiring specialized equipment and trained personnel. This impedes their use in the Point-of-Care (PoC) environment, where ease of operation, portability, and cost efficiency are prioritized. Small, robust electrochemical biosensors provide a means with which to analyze biomarkers in biological fluids in PoC environments. Optimized sensing surfaces, immobilization strategies, and efficient reporter systems are key to improving biosensor detection systems. The signal transduction and general performance of electrochemical sensors are determined by surface properties that link the sensing element to the biological sample. We analyzed the surface characteristics of screen-printed and thin-film electrodes using scanning electron microscopy and atomic force microscopy. An enzyme-linked immunosorbent assay (ELISA) was adapted for use in an electrochemical sensor. The robustness and reproducibility of the developed electrochemical immunosensor were investigated by detecting Neutrophil Gelatinase-Associated Lipocalin (NGAL) in urine. The sensor showed a detection limit of 1 ng/mL, a linear range of 3.5–80 ng/mL, and a CV% of 8%. The results demonstrate that the developed platform technology is suitable for immunoassay-based sensors on either screen-printed or thin-film gold electrodes.

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Section: Sensing Platform Evaluationmentioning

confidence: 70%

Section: Sensing Platform Evaluationmentioning

confidence: 71%

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

et al. 2023

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“…Lipocalin 2 (LCN 2) is a protein with a molecular weight of 25 kDa that mainly exists in the kidney, liver, epithelial cells, and other tissues. LCN 2 is considered a promising biomarker for the prediction of kidney-related diseases 111 . Kao et al 111 prepared NiO NPs-modified CeCuO x (NiO NPs/CeCuO x ) thin film-based biosensors.…”

Section: Application Of Electrochemical Protein Sensors In the Detect...mentioning

confidence: 99%

“…LCN 2 is considered a promising biomarker for the prediction of kidney-related diseases 111 . Kao et al 111 prepared NiO NPs-modified CeCuO x (NiO NPs/CeCuO x ) thin film-based biosensors. First, CeCuO x and NiO were deposited successively on a silicon substrate to prepare the electrode, and then, anti-LCN 2 was immobilized on its surface to detect the presence of LCN 2.…”

Section: Application Of Electrochemical Protein Sensors In the Detect...mentioning

confidence: 99%

Electrochemical protein biosensors for disease marker detection: progress and opportunities

Guo,

Zhao,

Huang

et al. 2024

Microsyst Nanoeng

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The development of artificial intelligence-enabled medical health care has created both opportunities and challenges for next-generation biosensor technology. Proteins are extensively used as biological macromolecular markers in disease diagnosis and the analysis of therapeutic effects. Electrochemical protein biosensors have achieved desirable specificity by using the specific antibody–antigen binding principle in immunology. However, the active centers of protein biomarkers are surrounded by a peptide matrix, which hinders charge transfer and results in insufficient sensor sensitivity. Therefore, electrode-modified materials and transducer devices have been designed to increase the sensitivity and improve the practical application prospects of electrochemical protein sensors. In this review, we summarize recent reports of electrochemical biosensors for protein biomarker detection. We highlight the latest research on electrochemical protein biosensors for the detection of cancer, viral infectious diseases, inflammation, and other diseases. The corresponding sensitive materials, transducer structures, and detection principles associated with such biosensors are also addressed generally. Finally, we present an outlook on the use of electrochemical protein biosensors for disease marker detection for the next few years.

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“…X-ray diffraction (XRD) is commonly employed to elucidate the perovskite crystalline structure. Ideally, perovskites of the form ABX 3 are arranged into a cubic cell, where the atoms are just touching each other, and the B-X distance is equal to a/2 and the A-X distance is 2a/2, where a is the cube unit cell length and the relationship between radii of ions holds RA + Rx = 2RB + Rx [113][114][115]. However, distorted structures with tetragonal, rhombohedral, or orthorhombic symmetry have been found, mainly arising from smaller A ions that induce a tilting of the BX 6 octahedra to optimize A-X bonding.…”

Section: Sensing Of Hydrogen Peroxide (H 2 O 2 )mentioning

confidence: 99%

Electrochemical Perovskite-Based Sensors for the Detection of Relevant Biomarkers for Human Kidney Health

Piñón-Balderrama,

Leyva-Porras,

Conejo-Dávila

et al. 2023

Chemosensors

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The importance of biomarker quantification in technology cannot be overstated. It has numerous applications in medical diagnostics, drug delivery, and the timely implementation of prevention and control strategies for highly prevalent diseases worldwide. However, the discovery of new tools for detection has become increasingly necessary. One promising avenue is the use of perovskite-based materials, which exhibit excellent catalytic activity and redox properties. These make them ideal candidates for the development of electrochemical sensors. In this review, the advances of purely non-enzymatic electrochemical detection of bio-analytes, with ABO3 perovskite form, are presented. The work allows the visualization of some of the modifications in the composition and crystal lattice of the perovskites and some variations in the assembly of the electrodes, which can result in systems with a better response to the detection of analytes of interest. These findings have significant implications for improving the accuracy and speed of biomarker detection, ultimately benefiting patients and healthcare professionals alike.

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Highly sensitive and selective electrochemical detection of lipocalin 2 by NiO nanoparticles/perovskite CeCuOx based immunosensor to diagnose renal failure

Cited by 8 publications

References 40 publications

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

Electrochemical protein biosensors for disease marker detection: progress and opportunities

Electrochemical Perovskite-Based Sensors for the Detection of Relevant Biomarkers for Human Kidney Health

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