Highly Sensitive and Selective Electrochemical Sensor for Detection of Escherichia coli by Using L-Cysteine Functionalized Iron Nanoparticles

Abstract

The World Health Organization states that every year more than 3.4 million human deaths occur as a result of waterborne diseases. Here, we reported an innovative and amino functionalized electrochemical biosensor for the rapid detection of Escherichia coli (E. coli). Functionalized Fe 3 O 4 NPs developed the sensor with L-cysteine, and cyclic voltammetry (CV) was employed to detect the bacteria. The results from this work are the first step toward the development of a portable water sensor that addresses the challenges of conventional time consuming and more expensive laboratory-based analyses. The detection of the targeted bacteria was performed with different bacteria to check the validity of the developed biosensor. Hence, the biosensor was highly selective to detect the E. coli from tap water samples. The signals current were increasing linearly while increasing the concentration of E. coli with the ranging from 10 1 to 10 5 CFU/mL with the linear correlation of (r 2 = 0.879). The proposed electrochemical biosensor detects the E. coli in a lower concentration of 10 1 CFU/mL and with the maximum range of 10 5 CFU/mL. The results from this work demonstrate the first step in a portable biosensor for the routine monitoring of microbial contamination from water.

Effective Removal of Arsenic in Water by Inductively Coupled Plasma Mass Spectrometry: Equilibrium and Kinetic Study

“…9. The XRD peaks were matched with reported literature [36,37]. All peaks are matched with the characteristics of magnetite material and it proposed the core-shell structure of synthesized iron oxide NPs as shown in Fig.…”

Review Article: Synthesis of Fe3O4 Nanoparticle and Its Application for Glassy Carbon Electrode Modification

“…It has been recognized that the activity of magnetite (Fe 3 O 4 ) NPs intensively depends on their shape, size and crystal phase. Particularly, shape and size have a striking impact on the properties of magnetite Fe 3 O 4 NPs with 1 to 100 nm because of the high surface area and their potential applications (Panhwar et al 2019).…”

“…Fe 3 O 4 NPs have excellent properties so that they be used in various sectors. For example, to modify the Glassy Carbon Electrode (GCE) Panhwar et al 2019). Many studies have been conducting to modify GCE with Fe 3 O 4 nanoparticles with different applications.…”

“…Then the nafion ball rn50 was dripped to stabilize the GCE surface. Nafion can protect the L-Cys/Fe3O4 NPs layer from the leaching process which can degrade the layer from the GCE surface (Panhwar et al 2019). An illustration of GCE modification using Fe 3 O 4 is shown in Figure 6.…”

Dual Responsive Disposable Electrode for the Enumeration of Escherichia coli in Whole Blood

“…The amino-functionalized electrochemical biosensor was constructed for the identification of E. coli in a lower concentration of 10 1 cfu/mL and the maximum detection range of 10 5 cfu/mL from water [10]. The electrochemical biosensor can work as a standalone instrument and transmit data through wi-fi for remote monitoring and control of the water quality as the enhancement of water treatment.…”