Highly sensitive and reliable detection of microRNA for clinically disease surveillance using SERS biosensor integrated with catalytic hairpin assembly amplification technology

Weng, Shuyun; Lin, Duo; Lai, Shuxia; Tao, Hong; Chen, Tong; Peng, Min; Qiu, Sufang; Feng, Shouhua

doi:10.1016/j.bios.2022.114236

Cited by 50 publications

(26 citation statements)

References 58 publications

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“…100% accurate clinical diagnosis of breast cancer was achieved by Weng et al for 60 samples [ 278 ]. In the following study, the targeted miRNA-21 and miRNA-155 were amplified by applying the isothermal catalytic hairpin assembly (CHA) strategy before SERS analysis.…”

Section: Sers Clinical Applicationsmentioning

confidence: 99%

“…The application of the signal second order peak of Si 936 cm −1 as an internal standard calibration enabled the reliable quantitative detection of miRNA with the correlation coefficient (R2) of 0.99. Thus, the ultralow concentration of miRNA-21 and miRNA-155 was detected (0.398 fM and 0.215 fM, respectively) and a 100% accurate diagnosis of breast cancer was performed [ 278 ].…”

Section: Sers Clinical Applicationsmentioning

confidence: 99%

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Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

et al. 2022

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This article compares the applications of traditional gold and silver-based SERS substrates and less conventional (Pd/Pt, Cu, Al, Si-based) SERS substrates, focusing on sensing, biosensing, and clinical analysis. In recent decades plethora of new biosensing and clinical SERS applications have fueled the search for more cost-effective, scalable, and stable substrates since traditional gold and silver-based substrates are quite expensive, prone to corrosion, contamination and non-specific binding, particularly by S-containing compounds. Following that, we briefly described our experimental experience with Si and Al-based SERS substrates and systematically analyzed the literature on SERS on substrate materials such as Pd/Pt, Cu, Al, and Si. We tabulated and discussed figures of merit such as enhancement factor (EF) and limit of detection (LOD) from analytical applications of these substrates. The results of the comparison showed that Pd/Pt substrates are not practical due to their high cost; Cu-based substrates are less stable and produce lower signal enhancement. Si and Al-based substrates showed promising results, particularly in combination with gold and silver nanostructures since they could produce comparable EFs and LODs as conventional substrates. In addition, their stability and relatively low cost make them viable alternatives for gold and silver-based substrates. Finally, this review highlighted and compared the clinical performance of non-traditional SERS substrates and traditional gold and silver SERS substrates. We discovered that if we take the average sensitivity, specificity, and accuracy of clinical SERS assays reported in the literature, those parameters, particularly accuracy (93–94%), are similar for SERS bioassays on AgNP@Al, Si-based, Au-based, and Ag-based substrates. We hope that this review will encourage research into SERS biosensing on aluminum, silicon, and some other substrates. These Al and Si based substrates may respond efficiently to the major challenges to the SERS practical application. For instance, they may be not only less expensive, e.g., Al foil, but also in some cases more selective and sometimes more reproducible, when compared to gold-only or silver-only based SERS substrates. Overall, it may result in a greater diversity of applicable SERS substrates, allowing for better optimization and selection of the SERS substrate for a specific sensing/biosensing or clinical application.

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Section: Sers Clinical Applicationsmentioning

confidence: 99%

Section: Sers Clinical Applicationsmentioning

confidence: 99%

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

et al. 2022

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“…The EM enhancement, as well as CE, require a very small distance between the analyte molecule and the metallic surface, and the amplification enhancement factor (EF) of SERS is on the order of 10 8 –10 14 . The SERS has been widely used these days as the best analytical tool in trace amounts [ 119 , 120 ].…”

Section: Microneedle Used For Glucose Monitoringmentioning

confidence: 99%

Microneedle-Based Glucose Sensor Platform: From Vitro to Wearable Point-of-Care Testing Systems

Regmi

et al. 2022

Biosensors

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Significant advanced have recently been made in exploiting microneedle-based (MN-based) diabetes devices for minimally invasive wearable biosensors and for continuous glucose monitoring. Within this emerging class of skin-worn MN-based sensors, the ISF can be utilized as a rich biomarker source to diagnose diabetes. While initial work of MN devices focused on ISF extraction, the recent research trend has been oriented toward developing in vivo glucose sensors coupled with optical or electrochemical (EC) instrumentation. This outlook highlights the essential characteristics of the sensing mechanisms, rational design, sensing properties, and applications. Finally, we describe the opinions about the challenge and prospects of optical and EC MN-based device platforms for the fabrication of wearable biosensors and their application potential in the future.

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“…As a class of nonenzymatic nucleic acid amplification circuits, catalytic hairpin assembly (CHA) has proved to be a versatile tool for amplifying and transducing signals. − In CHA, the isothermal hybridization of two DNA hairpin reactants is activated and progressed by introducing an invading oligonucleotide analyte (e.g., a target DNA segment). − Based on toehold-mediated branch migration, this targeting strand is displaced and recycled, making it available for another round of CHA, analogous to a catalyst, to guide more hairpin assembly events, which can yield distinct amplification and good turnover rate. ,− As such, CHA-based circuits have been engineered for the enzyme-free transduction of specific analyte binding in diverse signaling modalities, such as fluorescence or electrochemical current, ,, realizing the detection of nucleic acids, small molecules, and proteins. ,− In traditional CHA reactions, however, two hairpin species were positioned free in a homogeneous solution, such that the low local concentration of reactants brought slow reaction kinetics and insufficient conservation, particularly for ultralow target analytes. , Thus, it is desirable to speed up CHA operation and make it become more robust with respect to external disturbances. Remarkably, it has been demonstrated theoretically and experimentally that DNA supramolecular origami was employed as an exquisite platform to localize strand displacement reactions. − With the increase of local concentration, DNA strands transferring from one “docking site” were sped up considerably.…”

Section: Introductionmentioning

confidence: 99%

Target DNA-Activating Proximity-Localized Catalytic Hairpin Assembly Enables Forming Split-DNA Ag Nanoclusters for Robust and Sensitive Fluorescence Biosensing

Zhang

Yang

et al. 2022

Anal. Chem.

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Proximity-localized catalytic hairpin assembly (plCHA) is intriguing for rapid and sensitive assay of an HIV-specific DNA segment ( T *). Using template-integrated green Ag nanoclusters (igAgNCs) as emitters, herein, we report the first design of a T *-activated plCHA circuit that is confined in a three-way-junction architecture (3WJA) for the fluorescence sensing of T *. To this end, the T *-recognizable complement is programmed in a stem-loop hairpin (H1), and two split template sequences of igAgNCs are separately overhung contiguous to the paired stems of H1 and another hairpin (H2). The hybridization among H1, H2, and two single-stranded linkers (L1 and L2) allows the stable construction of 3WJA. Upon presenting the input T *, the 3WJA-localized plCHA is operated through toehold-mediated strand displacements of H1 and H2 reactants, and T * is rationally displaced and repeatably recycled, analogous to a specific catalyst, inducing more hairpin assembly events. Resultantly, the hybridized products enable the collective combination of two splits in the parent scaffold for hosting igAgNCs, outputting T *-dependent fluorescence response. Because of 3WJA structural confinement, the spatial proximity of two reactive hairpins yielded high local concentrations to manipulate the plCHA operation, achieving rapider reaction kinetics via T *-catalyzed recycling than typical catalytic hairpin assembly (CHA). This simple assay strategy would open the arena to develop various plCHA-based circuits capable of modulating the fluorescence emission of igAgNCs for applicable biosensing and bioanalysis.

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Highly sensitive and reliable detection of microRNA for clinically disease surveillance using SERS biosensor integrated with catalytic hairpin assembly amplification technology

Cited by 50 publications

References 58 publications

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

Microneedle-Based Glucose Sensor Platform: From Vitro to Wearable Point-of-Care Testing Systems

Target DNA-Activating Proximity-Localized Catalytic Hairpin Assembly Enables Forming Split-DNA Ag Nanoclusters for Robust and Sensitive Fluorescence Biosensing

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