Highly Sensitive and Rapid Assay of Substance P and Streptolysin O in Human Serum Using Immuno-liposomes and Gramicidin Channels

Sakamoto, Misato; Shoji, Atsushi; Sugawara, Masao

doi:10.2116/analsci.29.877

Cited by 4 publications

(3 citation statements)

References 46 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surface modifications have been employed to enhance the sensitivity of diagnostic applications. [144][145][146] Fast and visible readout has been achieved through a colorimetric strategy by incorporating certain additives that caused the aggregation of gold nanoparticles (AuNPs) upon activation of the analyte enzyme. The color changes of the AuNPs offered a signal transduction mechanism for naked-eye identification of the analyte, as shown in Figure 9B.…”

Section: Biosensors and Biodiagnosticsmentioning

confidence: 99%

Droplet-Templated Synthetic Cells

Shang

Zhao

2021

Matter

View full text Add to dashboard Cite

Section: Biosensors and Biodiagnosticsmentioning

confidence: 99%

Droplet-Templated Synthetic Cells

Shang

Zhao

2021

Matter

View full text Add to dashboard Cite

“…The gramicidin‐aided transmembrane signaling was further extended to include the procedure for enriching an analyte by agglutination/precipitation of immuno‐liposomes . A mixture of the immuno‐liposomes encapsulating a fluorescence dye (BCECF), gramicidin A and unknown concentration of an analyte is pre‐incubated in a solution and captured on an antibody‐modified cover slip, followed by the measurements of the fluorescence images after the removal of any excess liposomes.…”

Section: Transmembrane Signaling Across Spherical Bilayer‐lipid Membrmentioning

confidence: 99%

Transmembrane Signaling with Lipid‐Bilayer Assemblies as a Platform for Channel‐Based Biosensing

Sugawara

2017

The Chemical Record

Self Cite

View full text Add to dashboard Cite

Artificial and natural lipid membranes that elicit transmembrane signaling is are useful as a platform for channel-based biosensing. In this account we summarize our research on the design of transmembrane signaling associated with lipid bilayer membranes containing nanopore-forming compounds. Channel-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels, are embedded in planar and spherical bilayer lipid membranes to develop highly sensitive and selective biosensing methods for a variety of analytes. The membrane-bound receptor approach is useful for introducing receptor sites on both planar and spherical bilayer lipid membranes. Natural receptors in biomembranes are also used for designing of biosensing methods.

show abstract

“…53 As shown in Fig. 4, a mixture of the immunoliposomes encapsulating a pH-sensitive fluorescence dye BCECF, gramicidin A and a given concentration of SP (or SLO) is pre-incubated in a solution and captured on an anti-SP (or anti-SLO)-modified cover slip, followed by measurements of the fluorescence images after removal of excess liposomes.…”

Section: ·1 Immunoassay With a Liposome Arraymentioning

confidence: 99%

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

2014

Self Cite

View full text Add to dashboard Cite

119 IntroductionTranslocation of ions and small molecules across barriers of cellular membrane, lipid bilayers and solid supports is elicited by biological and artificial pore-forming compounds, which comprise protein, peptide and artificial molecules. Application of nanopores to biosensing holds much promise in developing highly sensitive methods because of their inherent ability of signal transduction and amplification.Nanopore-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels embedded in spherical and planar lipid bilayers ( Fig. 1), have been exploited as sensing elements for the development of biosensors and their uses for biosensing have been reviewed extensively. 1-13Receptor ionchannels, such as glutamate receptor ion channels, possess a 2014 © The Japan Society for Analytical Chemistry Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan Pore-forming compounds are attracting much attention due to the signal transduction ability for the development of highly sensitive biosensing. In this review, we describe an overview of the recent advances made by our group in the design of molecular sensing interfaces of spherical and planar lipid bilayers and natural bilayers. The potential uses of poreforming compounds, such as gramicidin and MCM-41, in lipid bilayers and natural glutamate receptor channels in biomembrane are presented.

show abstract

Highly Sensitive and Rapid Assay of Substance P and Streptolysin O in Human Serum Using Immuno-liposomes and Gramicidin Channels

Cited by 4 publications

References 46 publications

Droplet-Templated Synthetic Cells

Droplet-Templated Synthetic Cells

Transmembrane Signaling with Lipid‐Bilayer Assemblies as a Platform for Channel‐Based Biosensing

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

Contact Info

Product

Resources

About