Highly sensitive and multiplexed mass spectrometric immunoassay techniques and clinical applications

Xu, Shuting; Liu, Huwei; Bai, Yu

doi:10.1007/s00216-022-03945-4

Cited by 8 publications

(7 citation statements)

References 123 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the matrix may affect both immunological and mass spectrometric methods, the effects tend to be more prevalent in immunoassays because sample extraction or separation methods are less common. Immunoassays are generally commercially developed, while mass spectrometric methods are often laboratory-developed and can therefore be substantially different from one another [71][72][73]. Both methods suffer from this lack of standardization.…”

Section: Immunoassay or Mass Spectrometry-affordabilitymentioning

confidence: 99%

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Šoša¹

2023

Preprint

View full text Add to dashboard Cite

The literature was scoped to help place mass spectrometric (MS) platforms in the spotlight of the life sciences, as a tool of OMICS and OMICS 2.0 technologies. Moreover, today’s appliances are so handy, that they literally place a whole lab on a single chip. Methods of OMICS (and the most recently developed term OMICS 2.0) are tackled, having in mind their aptness for educational purposes, as well as their feasibility. This same topic was highlighted in many reviews that have been periodically published. The author’s initial interest was the forensic application of MS, but since these interests changed, terms such as “biosensors” were also examined, so the review included means of discovery of efficient biomarkers and clinical therapeutic targets. Aside from simply reviewing the research status and the future perspectives, this article tackles terms such as Society 5.0 and OMICS 2.0. However, within the framework of this review, physical chemistry, bioinformatics, or specific software solutions were not specifically studied. Since the topic of generating permanent collections of digital content for retrospective analysis was also reviewed, the need for loads of data – cloud storage was discussed in the context of MS. These large quantities of data are easier to operate with if generated by MS, rather than by any other commercially available assay. In this manner, the review has strengthened an in-depth understanding of the applications of MS.

show abstract

Section: Immunoassay or Mass Spectrometry-affordabilitymentioning

confidence: 99%

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Šoša¹

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Immunoassays are generally commercially developed, while mass spectrometric methods are often laboratory-developed and can therefore be substantially different from one another [66][67][68]. Both methods suffer from this lack of standardization.…”

Section: Immunoassay or Ms-affordabilitymentioning

confidence: 99%

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Šoša¹

2022

Preprint

View full text Add to dashboard Cite

The literature was scoped to help place mass spectrometric (MS) platforms in the spotlight of the life sciences, as a tool of OMICS and OMICS 2.0 technologies. These methods were discussed, and their feasibility was highlighted in many reviews that have been periodically published, in addition to the most recently developed term OMICS 2.0. This author’s primary interest was the forensic application of MS, but since these interests got broadened, terms such as “biosensors” were also included. So, means of discovery of efficient biomarkers and clinical therapeutic targets were also reviewed. However, within the framework of this review, physical chemistry, bioinformatics, or specific software solutions were not specifically scoped. Aside from simply reviewing the research status and the future perspectives, this article tackles terms such as Society 5.0 and OMICS 2.0. Since the topic of generating permanent collections of digital content for retrospective analysis was reviewed, what requires loads of data – cloud storage is discussed in the context of MS. In this manner, the review has strengthened an in-depth understanding of the applications of MS. These large quantities of data are easier to operate with if generated by MS, rather than by any other commercially available assay.

show abstract

“…Mass spectrometry (MS), as a new type of readout technique for immunoassay, has the advantages of good specificity and accurate quantification . Mass spectrometric immunoassay (MSIA) has become an emerging immunoassay method and has gradually received more and more attention . However, the problems of long analysis time, high instrumental cost, and insufficient sensitivity, whether using immunoaffinity columns or MALDI-TOF analysis, have hindered the further development of mass spectrometric immunoassay (MSIA).…”

Section: Introductionmentioning

confidence: 99%

Facile and Ultrasensitive Food Allergen Quantification Using Microzone Paper-Based Mass Spectrometric Immunoassay

Lu,

Xu,

Wang

et al. 2024

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Highly sensitive and rapid measurement of food allergens is essential to avoid unanticipated food allergies and to determine whether crosscontamination occurs in the food industry. Commercial immunoassay kits offer high specificity and convenience for allergen detection but still suffer limited quantitative sensitivity, accuracy, and stability based on the optical readout. In this work, a paper-based mass spectrometric immunoassay platform was constructed to achieve facile and highly sensitive quantification of peanut allergen, which combined the advantages of good specificity and accurate quantification from mass spectrometry and simplicity from a paperbased immunoassay. In this platform, a novel quaternary ammonium-based mass tag and a paper chip with a microzone were designed and developed, contributing to a large signal enhancement. This method was able to detect Ara h1 with a linear range of 0.1−100 ng mL −1 and a detection limit of 0.08 ng mL −1 in milk matrices. It has also been successfully applied to the accurate quantification of Ara h1 in six milk-related beverages, two biscuits, and two candy bars with complicated matrices and presented a low-concentration quantitation capability. This method gives a new type of mass spectrometric immunoassay for rapid and ultrasensitive allergen regulation in the food industry and for individual allergen differentiation research.

show abstract

Highly sensitive and multiplexed mass spectrometric immunoassay techniques and clinical applications

Cited by 8 publications

References 123 publications

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Facile and Ultrasensitive Food Allergen Quantification Using Microzone Paper-Based Mass Spectrometric Immunoassay

Contact Info

Product

Resources

About