Highly sensitive and label-free electrochemical biosensor based on gold nanostructures for studying the interaction of prostate cancer gene sequence with epirubicin anti-cancer drug

Abedi, Rokhsareh; Raoof, Jahan Bakhsh; Hashkavayi, Ayemeh Bagheri; Asghary, Maryam

doi:10.1016/j.microc.2021.106668

Cited by 24 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With higher scan speeds, the currents at the oxidation and reduction peaks both grow. As seen in Figure 2 c, a linear increase in the current as a function of root of scan rate is observed with a high R2 value of 0.99 for both bare and Aptamer modified bare electrode, which suggests diffusion-controlled behavior on the part of the electrode [ 51 , 52 ]. The effective working area of the electrodes was determined using the Randles-Sevcik relationship [ 53 ] as shown in Equation (1):

where I p is the peak current (A), n is the number of electrons transferred, A is the effective area of the electrode (cm 2) , D is the diffusion coefficient of [Fe(CN) 6 ] 3− considered to be 6.70 × 10 −6 cm 2 , C is the concentration (5 mM) and V is the scan rate (Vs −1 ).…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

View full text Add to dashboard Cite

Prostate cancer is one of the most frequently diagnosed male malignancies and can be detected by prostate-specific antigen (PSA) as a biomarker. To detect PSA, several studies have proposed using antibodies, which are not economical and require a long reaction time. In this study, we propose to use self-assembled thiolated single-strand DNA on electrodes functionalized by multi-walled carbon nanotubes (MWCNT) modified with gold nanoparticles (AuNPs) to realize a low-cost label-free electrochemical biosensor. In this regard, the PSA aptamer was immobilized via electrostatic adsorption on the surface of a screen-printed MWCNT/AuNPs electrode. The immobilization process was enhanced due to the presence of Au nanoparticles on the surface of the electrode. Surface characterization of the electrode at different stages of modification was performed by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) and contact angle for surface tension properties. The results showed an increase in surface roughness due to the absorbance of the aptamer on the electrode surfaces. The developed sensor has an extended linear range of 1–100 ng/mL, and a very low limit of detection down to 1 pg/mL. In addition, the reaction has a binding time of only five minutes on the developed electrodes. Investigations of the biosensor selectivity against several substances revealed an efficient selectivity for PSA detection. With this approach, low-cost biosensors with high sensitivity can be realized which have a wide linearity range and a low limit of detection, which are necessary for the early detection of prostate cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Hence, they are a perfect choice as transducers for biosensors. In this sense, we can find several works using spherical or other shape gold nanoparticle for DNA biosensors [ 7 , [18] , [19] , [20] ] but as far as we know there have not been reported the use of mixtures of gold nanomaterials (AuNMs) of different shapes (gold nanoparticles (AuNPs) and gold nanotriangles (AuNTs)) as platforms for DNA biosensor development.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

Gutiérrez-Gálvez

Caño

Menéndez-Luque

et al. 2022

Talanta

View full text Add to dashboard Cite

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy) 3 ] 2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy) 3 ] 2+ /CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.

show abstract

“…Compared to AuNPs, gold nanocubes (AuNC) tend to increase biosensor stability. In addition, the edges and vertices of AuNC provide higher surface energy, so there are more reaction sites available, resulting in more reactive facets [ 15 , 16 ]. Furthermore, Bollalla et al [ 17 ] have also observed that the shape of the gold nanomaterials has a crucial effect on the biocatalytic current related to the oxidation of fructose.…”

Section: Introductionmentioning

confidence: 99%

Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides

et al. 2022

View full text Add to dashboard Cite

Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment. Graphical abstract

show abstract

Highly sensitive and label-free electrochemical biosensor based on gold nanostructures for studying the interaction of prostate cancer gene sequence with epirubicin anti-cancer drug

Cited by 24 publications

References 60 publications

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides

Contact Info

Product

Resources

About