Highly sensitive and cost-effective ITO-based immunosensor system modified by 11-CUTMS: Analysis of SOX2 protein in real human serum

Özcan, Burcu; Sezgintürk, Mustafa Kemal

doi:10.1016/j.ijbiomac.2019.02.112

Cited by 14 publications

(3 citation statements)

References 28 publications

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“…When antibodies are synchronized with effective transducers, they can be produced and increase as immunosensors for identification of viruses, hormones, pesticides, bacteria, medications and molecules causing environmental pollution . The advantage of immunosensors is that they are based on the antigen‐antibody interaction, and have the capacity to be used for more than one aim, making them promising biosensors for advancing technology .…”

Section: Introductionmentioning

confidence: 68%

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

Tarimeri

Sezgintürk

2020

Electroanalysis

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In this study, an ITO (indium tin oxide) based biosensor was constructed to detect SOX2. SOX2 helps the regulation of cell pluripotency and is closely related to early embryonic development, neural and sexual differentiation. SOX2 is amplified and overexpressed in some malignant tumors such as squamous cell, lung, prostate, breast, esophageal cell, colon, ovarian, glioblastoma, pancreatic cancer, gastric cancer, head and neck squamous cell carcinoma. To generate a hydroxylated clean electrode surface, ITO electrodes were treated with NH4OH/H2O2/H2O. Later, ITO‐PET electrode surfaces were modified with 3‐glycidoxypropyl trimethoxysilane (3‐GOPS). Then, Anti‐SOX2 was covalently immobilized onto the electrode surfaces. 3‐GOPS concentration, Anti‐SOX2 concentration and incubation time, SOX2 incubation time were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized in order to follow up the immobilization processes and the optimization steps of the biosensor. To characterize the analytical properties of constructed immunosensor; linear range, repeatability, reproducibility and regeneration studies were investigated. The linear range of the immunosensor was detected as 0.625 pg/mL–62.5 pg/mL. Square wave voltammetry technique was also applied to the biosensor. Storage life of the biosensor was determined for identifying the possible usability of the biosensor in clinical field. Finally, the designed biosensor was applied to the real human serum samples. The results obtained with the presented biosensor were also compared with ELISA results.

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Section: Introductionmentioning

confidence: 68%

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

Tarimeri

Sezgintürk

2020

Electroanalysis

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“…Impedance spectroscopy is an effective method for investigating electrochemical systems . EIS, unlike other electrochemical methods, can be utilized in both volume studies and interfacial processes associated with time constants (from minutes to microseconds). , As can be seen in Figure A, the semidiameter of the impedance curve is very low because the surface of the GP electrode is highly conductive.…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05–25 pg mL–1) and a low limit of detection (0.0033 pg mL–1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL–1), creatine kinase (50 pg mL–1), parathyroid hormone (50 pg mL–1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels.

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“…Using ITO-PET electrode in the designed biosensor; it has provided very important advantages such as being economical, enabling effective covalent immobilizations, high stability, easy miniaturization and being suitable for disposable design. [10][11][12] For effective immobilization of anti-ST2 to the ITO-PET surface, a suitable modification method must be selected. For this purpose, the silane agent method was applied in this study.…”

Section: Introductionmentioning

confidence: 99%

A Novel Disposable Immunosensor for Early Diagnosis of Cardiovascular Diseases

2022

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Cardiovascular diseases are highly common cause of mortality and morbidity. It is envisaged that there is a relationship between cardiovascular diseases and ST2 (Suppression of Tumorigenicity 2) concentration of protein. In patients with cardiac insufficiency, the ST2 concentration was reported as higher than people without cardiac insufficiency. In this study ITO-PET sheets (indium tin oxide covered terephthalate) was used as a working electrode for the fabrication of the immunosensor for ST2 protein. In the optimization experiments, important parameters responsible for biosensor fabrica-tion have been optimized. After determining optimal conditions, reproducibility, repeatability, regeneration possibility, and analytical properties such as storage life are determined. The immunosensor showed a linear detection range for ST2 between 1-1500 fg/mL with a LOD value of 3.98 fg/mL. We also carried out SFI (single frequency impedance) experiments to monitor the specific interaction between ST2 and anti-ST2, simultaneously. The developed immunosensor was successively applied to the determination of ST2 in commercial human serum sample.

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Highly sensitive and cost-effective ITO-based immunosensor system modified by 11-CUTMS: Analysis of SOX2 protein in real human serum

Cited by 14 publications

References 28 publications

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

A Novel Disposable Immunosensor for Early Diagnosis of Cardiovascular Diseases

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