Highly selective red- and green-emitting two-photon fluorescent probes for cysteine detection and their bio-imaging in living cells

Abstract

Two highly selective two-photon fluorescent probes for cysteine over homocysteine, N-acetyl-L-cysteine, dithiothreitol, glutathione and other amino acids, and their fluorescent imaging in living cells have been shown.

An ‘OFF–ON’ fluorescent probe for specially recognize on Cys and its application in bioimaging

“…Up to date, the design of highly selective probes for Cys, Hcy, and GSH is still a challenge and very few examples have been reported so far. Particularly, only a few sensors can detect Cys over Hcy and GSH in biological systems [42][43][44][45][46]. In our group, we always expect to obtain the specific probe for distinguishing among Cys, Hcy and GSH.…”

Push–pull fluorophores with viscosity dependent and aggregation induced emissions insensitive to polarity

“…A C C E P T E D ACCEPTED MANUSCRIPT 2 applications in various fields like organic light emitting diodes (OLED) [4], dye-sensitized solar cells [5,6], non linear optics [7], photoconductors [8], solid state lasers [9], and bioimaging [10]. The carbazole derivatives are also appealing molecules because of their intense luminescence and electron efficiency [11].…”

A ratiometric fluorescent probe for cysteine and its application in living cells

“…Certainly, many thiol probes are reported based on the inherent nucleophilicity of sulfydryl group which commonly consist in Cys, GSH and homocysteine (Hcy). The different mechanisms such as conjugate addition/cyclization reaction [12][13][14], aldehyde addition [15][16][17], cleavage reaction [18][19][20], and Michael addition [21][22][23][24] and so on [25][26][27][28][29][30] have also been proposed. However, it is seriously hampered to distinguish Cys from Hcy and GSH because of their similar structure.…”