Highly selective optical sensor N/S-doped carbon quantum dots (CQDs) for the assessment of human chorionic gonadotropin β-hCG in the serum of breast and prostate cancer patients

AlZahrani, Yasmeen M.; Alharthi, Salha; Alghamdi, Hanan M.; Youssef, A.M.; Ahmed, Shaheda; Mohamed, Ekram H.; Mahmoud, Safwat A.; Attia, M. S.

doi:10.1039/d3ra01570j

Cited by 2 publications

(4 citation statements)

References 51 publications

(65 reference statements)

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“…The sensor, engineered to emit blue luminescence at specific wavelengths, responds to the varying concentrations of beta-hCG, offering a sensitive method for the assessment of this cancer biomarker in serum samples. This innovation promises the highly selective and sensitive detection of beta-hCG levels with low detection thresholds (Figure 5) [83].…”

Section: Quantum Dot Applications In Hormone and Protein Biomarker De...mentioning

confidence: 99%

“…biomarker in serum samples. This innovation promises the highly selective and sensitive detection of beta-hCG levels with low detection thresholds (Figure 5) [83]. An electrochemical biosensor was devised for the ultrasensitive detection of MCF-7 breast cancer cells in human serum employing nitrogen-doped graphene quantum dots (NGQDs) and phytohemagglutinin-L (PHA-L) deposited on screen-printed electrodes.…”

Section: Quantum Dot Applications In Hormone and Protein Biomarker De...mentioning

confidence: 99%

“…Quantum Dot Synthesis and Characterization: This involves assessing optical, photophysical, and surface properties to optimize the QDs for specific applications [5,34,39,66,67,83,88,[90][91][92]112,119]. Advanced characterization techniques such as transmission electron microscopy (TEM), Scanning Electron microscopy (SEM), Fouriertransform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-Visible spectroscopy (UV-Vis), photoluminescence (PL), high-resolution transmission electron microscopy (HRTEM), Field Emission Scanning Electron microscopy (FESEM), dynamic light scattering (DLS), photoluminescence spectroscopy (PL), and atomic force microscopy (AFM), are crucial for uncovering detailed structural and functional insights [5,14,15,30,34,35,79,[88][89][90]112].…”

Section: Testing and Evaluation Of The Qd Productsmentioning

confidence: 99%

“…Shows results achieved for luminescence emission spectra of N/S-dopes CQDs in human chorionic gonadotropin β-hCG in the serum of the patients with breast and prostate cancer created from a low-cost highly selective method in the presence of different concentrations of β-hCG in DMSO at λ ex = 288 nm. The method was successful in measuring the PCT in samples, used to assess biomarkers for cancer diseases in the body, achieving a dynamic range of 1.35-22.95 mU mL −1 , with the limit of detection (LOD) and quantitation limit of detection (LOQ) of 0.235 and 0.670 mU mL −1 , respectively[83].…”

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confidence: 99%

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Quantum Dot Research in Breast Cancer: Challenges and Prospects

Omidian,

Wilson,

Cubeddu

2024

Materials

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The multifaceted role of quantum dots (QDs) in breast cancer research highlights significant advancements in diagnostics, targeted therapy, and drug delivery systems. This comprehensive review addresses the development of precise imaging techniques for early cancer detection and the use of QDs in enhancing the specificity of therapeutic delivery, particularly in challenging cases like triple-negative breast cancer (TNBC). The paper also discusses the critical understanding of QDs’ interactions with cancer cells, offering insights into their potential for inducing cytotoxic effects and facilitating gene therapy. Limitations such as biocompatibility, toxicity concerns, and the transition from laboratory to clinical practice are critically analyzed. Future directions emphasize safer, non-toxic QD development, improved targeting mechanisms, and the integration of QDs into personalized medicine, aiming to overcome the current challenges and enhance breast cancer management.

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Section: Quantum Dot Applications In Hormone and Protein Biomarker De...mentioning

confidence: 99%

Section: Quantum Dot Applications In Hormone and Protein Biomarker De...mentioning

confidence: 99%

Section: Testing and Evaluation Of The Qd Productsmentioning

confidence: 99%

mentioning

confidence: 99%

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Quantum Dot Research in Breast Cancer: Challenges and Prospects

Omidian,

Wilson,

Cubeddu

2024

Materials

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Designing an Electrochemical Biosensor Based on Voltammetry for Measurement of Human Chorionic Gonadotropin

Samare-Najaf,

Dehghanian,

Asadikaram

et al. 2024

Journal of Medical Signals &Amp; Sensors

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Background: Human chorionic gonadotropin (hCG) is a polypeptide hormone synthesized during pregnancy and is also upregulated in some pathologic conditions such as certain tumors. Its measurement is essential for diagnosing pregnancy and malignancies. Despite numerous attempts to introduce an accurate method capable of detecting hCG levels, several limitations are found in previous techniques. This study aimed to address the limitations of current hCG assay methods by designing an electrochemical biosensor based on voltammetry for the rapid, selective, inexpensive, and sensitive measurement of hCG levels. Methods: A carbon paste electrode was prepared and functionalized by para-aminobenzoic acid. The primary anti-β-hCG monoclonal antibody was immobilized on the electrode surface by activating the carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide solutions. The study also involved optimizing parameters such as the time for primary antibody fixation, the time for hCG attachment, and the pH of the hydrogen peroxide solution to maximize the biosensor response. Different concentrations of hCG hormone were prepared and loaded on the electrode surface, the secondary antibody labeled with HRP enzyme was applied, thionine in phosphate-buffered saline solution was placed on the electrode surface, and the differential pulse electrical signal was recorded. Results: The linear range ranged from 5 to 100 mIU/ml, and the limit of detection was calculated as 0.11 mIU. The relative standard deviation was 3% and 2% for five repeated measurements of commercial standard samples with concentrations of 2 and 20 mIU/mL, respectively. The percent recovery was obtained from 98.3% to 101.5%. Conclusion: The sensor represents a promising advancement in hCG level measurement, offering a potential solution to overcome the existing limitations in current diagnostic strategies. Simple and inexpensive design, detecting hCG in its important clinical range during early pregnancy, and successful measurement of hCG in real serum samples are the advantages of this sensor.

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Highly selective optical sensor N/S-doped carbon quantum dots (CQDs) for the assessment of human chorionic gonadotropin β-hCG in the serum of breast and prostate cancer patients

Abstract: A low-cost, accurate, and highly selective method was used for the assessment of the human chorionic gonadotropin β-hCG in the serum of breast and prostate cancer patients.

Cited by 2 publications

References 51 publications

Quantum Dot Research in Breast Cancer: Challenges and Prospects

Quantum Dot Research in Breast Cancer: Challenges and Prospects

Designing an Electrochemical Biosensor Based on Voltammetry for Measurement of Human Chorionic Gonadotropin

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