Highly-selective optical filter for NADH fluorescence detection in multiphoton microscopy
R. B. Freitas,
M. J. L. F. Rodrigues,
S. Pimenta
et al.
Abstract:Colorectal cancer (CRC) is a pressing global health concern, emphasizing the need for early detection tools. In this study an optical filter for precise detection of nicotinamide adenine dinucleotide (NADH) fluorescence via two-photon excitation fluorescence (TPEF) was developed. Fabricated with silicon dioxide and titanium dioxide thin films in a Fabry-Perot structure, the filter achieved a peak transmittance of about 95% at 483 nm, with a 12 nm full-width at half maximum. TPEF measurements using a tailored s… Show more
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