Highly Selective Fluorescent Probe for the Sensitive Detection of Inorganic and Organic Mercury Species Assisted by H₂O₂

Abstract

Mercury (Hg) and its derivatives pose a serious threat to the environment and human health because of their durability, easy transference and high biological accumulation. Thus, the development of methods for selective and sensitive determination of Hg 2+ is very important to understand its distribution and implement more detailed toxicological studies.Herein, we report a simple 4-hydroxynaphthalimide-derived fluorescent probe (1) for the detection of both inorganic and organic mercury species in aqueous solution. Probe 1 could quantificationally detect mercury species by fluorescence spectroscopy with high selectivity and sensitivity. Importantly, probe 1 could serve as a "naked-eye" indicator for mercury species and exhibited a large fluorescent enhancement with the help of hydrogen peroxide (H 2 O 2 ). Additionally, the mechanism of the reaction between probe 1 and mercury species was confirmed using 1 H-NMR and ESI-MS. And, analytical applications of probe 1 to the river water samples further demonstrated that it provided an excellent method for the determination of mercury species in the environment.

Construction of a lysosome-targetable ratiometric fluorescent probe for H2O2tracing and imaging in living cells and an inflamed model

“…Materials and equipment are described in the ESI. † Synthesis of 5 and dye 2 (NPT) 5 and 2 was synthesized according to the previous methods, 38 and the synthesis steps were shown in Fig. 1.…”

“…[18][19][20][21][22] Recently, a lot of H 2 O 2 uorescent probes have been successfully developed for the detection and imaging of H 2 O 2 . [23][24][25][26][27][28][29][30][31][32] However, most of the reported H 2 O 2 uorescent probes were constructed from enhancing or reducing the output signal by a single-channel, which affected by the concentration of probe molecules, environmental factors, instrumental efficiency, etc. In contrast, ratiometric uorescent probes have a built-in correction of the two emission bands, the ratiometric uorescent probes can eliminate most, if not all, such interference.…”

A Highly Selective and Ultrasensitive Fluorescent Probe for Monitoring Hg2+ and Its Applications in Real Water Samples

“…[26][27][28][29][30][31] In addition, most of these reported probes detect Hg 2+ with difficulty in the aqueous solution, and have low sensitive in actual operations, which make the detection become harder, and even failure. [32][33][34][35][36][37] Therefore, it is vital to research new fluorescent probes with water-solubility, high sensitivity and selectivity to detect mercury ions.…”

A carbonothioate‐based highly selective fluorescent probe with a large Stokes shift for detection of Hg2+

“…[1][2][3][4] Once it has entered the organism, it is difficult to be excreted, and will have a serious effect on the central nervous, endocrine systems, kidney, respiratory system, blood, etc. [5][6][7] Based on the standard 2 ppb (0.01 μM) of mercury contamination in water which was set by the Environment Protection Agency (EPA), [8,9] very small amount of mercury will cause serious harm to human, so it is very important to develop simple and effective methods for sensitive and selective detection of Hg 2+ . [10] Many methodologies have been used to detect Hg 2+ , such as atomic absorption spectroscopy, electrochemical analysis, inductively coupled plasma mass spectrometry, optical probes and so on.…”