Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles

Qiu, Suyan; Lin, Zhenyu; Zhou, Yaomin; Wang, Donggen; Yuan, Lijuan; Wei, Yihua; Dai, Tingcan; Luo, Lijun; Chen, Guonan

doi:10.1039/c4an02106a

Cited by 48 publications

(27 citation statements)

References 37 publications

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“…A chromogenic reaction occurs once the galactose moiety is cleaved by β-galactosidase. Qiu et al [ 34 ] reported that the lysozyme-capped Au nanoparticle’s colorimetric sensing system enabled the naked-eye detection of about 3.7 log CFU/mL of Bacillus subtilis suspended inside a 3-( N -morpholino)-propanesulfonic acid (MOPS) buffer. They also concluded that their colorimetric sensor is applicable for the rapid and specific microbial detection assay of complex samples.…”

Section: Colorimetric Methodsmentioning

confidence: 99%

“…Although the aforementioned studies demonstrated cost effectiveness, simplicity, ease of operation, and the rapid response of the colorimetric sensor, significant weaknesses and limitations were also observed. First and foremost, the researchers [ 32 , 33 , 34 ] suspended their target microorganisms in buffer or water. These solutions are typically cleaner and less complex than food homogenates.…”

Section: Colorimetric Methodsmentioning

confidence: 99%

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Current Technical Approaches for the Early Detection of Foodborne Pathogens: Challenges and Opportunities

Cho

2017

IJMS

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The development of novel and high-tech solutions for rapid, accurate, and non-laborious microbial detection methods is imperative to improve the global food supply. Such solutions have begun to address the need for microbial detection that is faster and more sensitive than existing methodologies (e.g., classic culture enrichment methods). Multiple reviews report the technical functions and structures of conventional microbial detection tools. These tools, used to detect pathogens in food and food homogenates, were designed via qualitative analysis methods. The inherent disadvantage of these analytical methods is the necessity for specimen preparation, which is a time-consuming process. While some literature describes the challenges and opportunities to overcome the technical issues related to food industry legal guidelines, there is a lack of reviews of the current trials to overcome technological limitations related to sample preparation and microbial detection via nano and micro technologies. In this review, we primarily explore current analytical technologies, including metallic and magnetic nanomaterials, optics, electrochemistry, and spectroscopy. These techniques rely on the early detection of pathogens via enhanced analytical sensitivity and specificity. In order to introduce the potential combination and comparative analysis of various advanced methods, we also reference a novel sample preparation protocol that uses microbial concentration and recovery technologies. This technology has the potential to expedite the pre-enrichment step that precedes the detection process.

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Section: Colorimetric Methodsmentioning

confidence: 99%

Section: Colorimetric Methodsmentioning

confidence: 99%

Current Technical Approaches for the Early Detection of Foodborne Pathogens: Challenges and Opportunities

Cho

2017

IJMS

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“…The attachments (or detachments) of specific proteins to (or from) AuNP in the presence of competing interactions with target analytes can be monitored using color transitions of test solutions. For example, a facile method for detecting Bacillus subtilis was developed using AuNP complexed with lysozyme, which normally imparts a purple color [51]. Furthermore, lysozyme has selective affinity for peptidoglycans of gram-positive bacterial cell walls.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

“…Detection of Bacillus subtilis using gold nanoparticle/lysozyme supramolecules.Reproduced with permission from The Royal Society of Chemistry[51].…”

mentioning

confidence: 99%

Analytical Applications of Nanomaterials in Monitoring Biological and Chemical Contaminants in Food

Lim

Kim

2016

Journal of Microbiology and Biotechnology

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The detection of food pathogens is an important aspect of food safety. A range of detection systems and new analytical materials have been developed to achieve fast, sensitive, and accurate monitoring of target pathogens. In this review, we summarize the characteristics of selected nanomaterials and their applications in food, and place focus on the monitoring of biological and chemical contaminants in food. The unique optical and electrical properties of nanomaterials, such as gold nanoparticles, nanorods, quantum dots, carbon nanotubes, graphenes, nanopores, and polydiacetylene nanovesicles, are closely associated with their dimensions, which are comparable in scale to those of targeted biomolecules. Furthermore, their optical and electrical properties are highly dependent on local environments, which make them promising materials for sensor development. The specificity and selectivity of analytical nanomaterials for target contaminants can be achieved by combining them with various biological entities, such as antibodies, oligonucleotides, aptamers, membrane proteins, and biological ligands. Examples of nanomaterial-based analytical systems are presented together with their limitations and associated developmental issues.

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“…Recently, considerable efforts have been devoted to the detection of protein by homogeneous assays, such as colorimetric assays based on various nanomaterials including gold nanoparticles, carbon nanotubes, metal nanocomposites, and carbon nanoparticles . These mix‐and‐measure techniques that have no need for separation steps significantly simplify operation and enhance the detection efficiency …”

Section: Introductionmentioning

confidence: 99%

A New Colorimetric Platform for Protein Detection Based on Recognition‐Induced Cascade of Polymeric Nanoparticles Disassembly

Fang

Liu

et al. 2018

Macromolecular Bioscience

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Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the molecular recognition between protein and the ligand on the surface of the polymeric micelles as the driving force to trigger the readout of the detection signal. The polymeric micelles formed via the self-assembly of the amphiphilic block polymer biotin-labeled poly(ethylene glycol)-block-poly(3-acryl aminophenylboronic acid) are endowed with colorful feature by incorporation of alizarin red S (ARS) into the hydrophobic core. Based on the response to streptavidin recognition, these micelles are further disintegrated through the competitive binding of α-cyclodextrin with boronic acid for disassociation of ARS, which achieves orange-yellow to pink-purple transition in 2 h. This work will open the way to develop one new mix-and-measure, visual, and homogeneous assay.

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Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles

Cited by 48 publications

References 37 publications

Current Technical Approaches for the Early Detection of Foodborne Pathogens: Challenges and Opportunities

Current Technical Approaches for the Early Detection of Foodborne Pathogens: Challenges and Opportunities

Analytical Applications of Nanomaterials in Monitoring Biological and Chemical Contaminants in Food

A New Colorimetric Platform for Protein Detection Based on Recognition‐Induced Cascade of Polymeric Nanoparticles Disassembly

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