Highly selective biomarkers for pesticides developed in Eisenia fetida using SELDI-TOF MS

Park, Doo-San; Jeon, Hwang‐Ju; Park, Eun-Sil; Bae, In Kyung; Kim, Yong-Eun; Lee, Sung‐Eun

doi:10.1016/j.etap.2014.12.020

Cited by 10 publications

(7 citation statements)

References 12 publications

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“…4B). As also reported in other papers in which the effects of environmental pollutant on protein expression were evaluated by SELDI-TOF-MS, it is possible to efficiently discriminate between samples exposed to different environmental conditions on the basis of a set of differentially expressed peaks (Moon et al, 2014;Park et al, 2015).…”

Section: Seldi-tof-ms Protein Profilingmentioning

confidence: 67%

“…Traditional endpoints, such as growth curves (Moon et al, 2014;Morelli et al, 2013Morelli et al, , 2012 or other commonly used biomarkers such as the antioxidant molecules (Lee et al, 2013(Lee et al, , 2008Mukherjee et al, 2014) or metal chelating compounds (Gomes et al, 2011), which are reported to be activated as a response to stress induced by nanoparticles, can be useful parameters, but are too unspecific for the kind of stress and quite time-consuming to be measured. In recent papers (Moon et al, 2014;Park et al, 2015), it was demonstrated the ability of SELDI-TOF-MS to discriminate, by protein profiles, even slightly different forms of the same kind of pollutant, thus confirming the superior capability of SELDI in detecting biomarkers that can be used as an early alert for the presence of toxic substances in the environment.…”

Section: Seldi-tof-ms Protein Profilingmentioning

confidence: 85%

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A SELDI-TOF approach to ecotoxicology: comparative profiling of low molecular weight proteins from a marine diatom exposed to CdSe/ZnS quantum dots

Scebba

Tognotti

Presciuttini

et al. 2016

Ecotoxicology and Environmental Safety

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Quantum dots (QDs), namely semiconductor nanocrystals, due to their particular optical and electronic properties, have growing applications in device technology, biotechnology and biomedical fields. Nevertheless, the possible threat to human health and the environment have attracted increasing attention as the production and applications of QDs increases rapidly while standard evaluation of safety lags. In the present study we performed proteomic analyses, by means of 2D gel electrophoresis and Surface Enhanced Laser Desorption Ionization-Time of Flight-Mass Spectrometry (SELDI-TOF-MS). We aimed to identify potential biomarkers of exposure to CdSe/ZnS quantum dots. The marine diatom Phaeodactylum tricornutum exposed to 2.5nM QDs was used as a model system. Both 2DE and SELDI showed the presence of differentially expressed proteins. By Principal Component Analysis (PCA) we were able to show that the differentially expressed proteins can discriminate between exposed and not exposed cells. Furthermore, a protein profile specific for exposed cells was obtained by SELDI analysis. To our knowledge, this is the first example of the application of SELDI technology to the analysis of microorganisms used as biological sentinel model of marine environmental pollution.

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Section: Seldi-tof-ms Protein Profilingmentioning

confidence: 67%

Section: Seldi-tof-ms Protein Profilingmentioning

confidence: 85%

A SELDI-TOF approach to ecotoxicology: comparative profiling of low molecular weight proteins from a marine diatom exposed to CdSe/ZnS quantum dots

Scebba

Tognotti

Presciuttini

et al. 2016

Ecotoxicology and Environmental Safety

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“…[19,20] We recently analyzed lipids and small proteins using MALDI-TOF MS/MS and SELDI-TOF MS in chlorpyrifos-and endosulfan-exposed fish samples. [14,15,21] In this study, MALDI-TOF MS/MS identified phospholipids that had been differentially biosynthesized after exposure to chlorpyrifos in Japanese rice fish. [21] In addition, a phospholipid with a mass ion of 556.32 significantly decreased in concentration after exposure to chlorpyrifos in Japanese medaka.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…[5] Its toxicity to fish has been reported at LC 50 values lower than those of terrestrial animals. [14,15] In tiger earthworms, Eisenia fetida, endosulfan showed acute toxicity with an LC 50 value of 7.7 g L −1 following direct contact. [15] In Japanese medaka (Oryzias latipes), endosulfan exhibited toxicity with an LC 50 value of 22.18 μg L −1…”

Section: Introductionmentioning

confidence: 99%

Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Moon

Jeon

Nam

et al. 2016

Chemical Speciation & Bioavailability

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Endosulfan has been listed as a persistent organic pollutant, and is frequently found in agricultural environments during monitoring processes owing to its heavy use and persistent characteristics. This study was conducted to understand the effects of endosulfan on the development of zebrafish (Danio rerio) embryos by exposing them to a specific range of endosulfan concentrations. Exposing zebrafish embryos to endosulfan for 96 h yielded no acute toxicity until the concentration reached 1500 μg L −1 , whereas malformed zebrafish larvae developed severely curved spines and shortened tails. About 50% of zebrafish larvae were malformed when exposed to 600 μg L −1 of endosulfan. Comparative gene expression using realtime quantitative polymerase chain reaction was assessed using endosulfan-exposed zebrafish embryos. CYP1A and CYP3A were significantly enhanced in response to endosulfan treatment. Two genes, acacb and fasn, encoding acetyl-CoA carboxylase b and fatty acid synthase proteins, respectively, were also up-regulated after treating zebrafish embryos with endosulfan. These genes are also involved in fatty acid biosynthesis. The genes encoding vitellogenin and Hsp70 increased in a concentration-dependent manner in embryos. Finally, biochemical studies showed that acetylcholinesterase activity was reduced, whereas glutathione S-transferase and carboxylesterase activities were enhanced in zebrafish embryos after endosulfan treatment. These biochemical and molecular biological differences might be used for tools to determine contamination of endosulfan in the aquatic environment.

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“…specific for human cancers (Silsirivanit et al 2014), or for endosulfan exposure in Japanese rice fish (Oryzias latipes) (Leeet al 2013). In a recent paper, selective protein biomarkers for 6 pesticides (captan, carbaryl, carbofuran, and α-endosulfan chlorpyrifos, propoxur) were found in E. fetida, by means of SELDI-TOF MS technology(Park et al 2015).…”

mentioning

confidence: 99%

Fungi as a toolbox for sustainable bioremediation of pesticides in soil and water

Spina

Cecchi

Landínez-Torres

et al. 2018

Plant Biosystems - An International Journal Dealing with all As

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Pesticides can help reduce yield losses caused by pests, pathogens, and weeds, but their 22 overuse causes serious environmental pollution. They are persistent in the environment and 23 are biomagnified through the food chain, becoming a serious health hazard for humankind. 24 Bioremediation, where microbes are used to degrade pesticides in situ, is a useful technology. 25 This review summarizes data on the fungi involved in the biodegradation of chemical 26 pesticides; and their application in soil and water bioremediation. Indications for future 27 studies in this field are given.

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Highly selective biomarkers for pesticides developed in Eisenia fetida using SELDI-TOF MS

Cited by 10 publications

References 12 publications

A SELDI-TOF approach to ecotoxicology: comparative profiling of low molecular weight proteins from a marine diatom exposed to CdSe/ZnS quantum dots

A SELDI-TOF approach to ecotoxicology: comparative profiling of low molecular weight proteins from a marine diatom exposed to CdSe/ZnS quantum dots

Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Fungi as a toolbox for sustainable bioremediation of pesticides in soil and water

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