Highly selective and sensitive detection of glutamate by an electrochemical aptasensor

Wu, Changtong; Barkova, Daria; Комарова, Н. В.; Offenhäusser, Andreas; Andrianova, Mariia; Hu, Ziheng; Kuznetsov, Alexander; Mayer, Dirk

doi:10.1007/s00216-021-03783-w

Cited by 18 publications

(13 citation statements)

References 82 publications

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“…Aptamers are able to selectively capture certain molecules such as glutamate, dopamine, and serotonin and the capture event can be transduced electrically. [111][112][113] To also release neurotransmitters into the tissue, slow-release hydrogels could be combined into the neural interface, alternatively, a microfluidic dispensing system running in parallel with the interface, [114] or could even use ultrasound-triggered aptamers to release the chemical compound on-demand. [115] The more dimensions along which the neural network is measured and stimulated will yield ever more sophisticated interoperation possibilities that could help both in therapies and in the fundamental understanding of neuronal function (Figure 3).…”

Section: Multifunctionalitymentioning

confidence: 99%

“…Aptamers are able to selectively capture certain molecules such as glutamate, dopamine, and serotonin and the capture event can be transduced electrically. [ 111–113 ] To also release neurotransmitters into the tissue, slow‐release hydrogels could be combined into the neural interface, alternatively, a microfluidic dispensing system running in parallel with the interface, [ 114 ] or could even use ultrasound‐triggered aptamers to release the chemical compound on‐demand. [ 115 ]…”

Section: Next Generation Devicesmentioning

confidence: 99%

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Toward the Next Generation of Neural Iontronic Interfaces

Forró

Musall

Montes³

et al. 2023

Adv Healthcare Materials

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Neural interfaces are evolving at a rapid pace owing to advances in material science and fabrication, reduced cost of scalable complementary metal oxide semiconductor (CMOS) technologies, and highly interdisciplinary teams of researchers and engineers that span a large range from basic to applied and clinical sciences. This study outlines currently established technologies, defined as instruments and biological study systems that are routinely used in neuroscientific research. After identifying the shortcomings of current technologies, such as a lack of biocompatibility, topological optimization, low bandwidth, and lack of transparency, it maps out promising directions along which progress should be made to achieve the next generation of symbiotic and intelligent neural interfaces. Lastly, it proposes novel applications that can be achieved by these developments, ranging from the understanding and reproduction of synaptic learning to live‐long multimodal measurements to monitor and treat various neuronal disorders.

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Section: Multifunctionalitymentioning

confidence: 99%

Section: Next Generation Devicesmentioning

confidence: 99%

Toward the Next Generation of Neural Iontronic Interfaces

Forró

Musall

Montes³

et al. 2023

Adv Healthcare Materials

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“…The binding affinity of Capture-SELEX-generated aptamers for small molecules can be identified by a novel label-free fluorescence SGI assay [ 24 , 68 , 70 , 73 , 83 ]. SGI is a fluorescent nucleic-acid-intercalating dye that was introduced in the 1990s and is widely applied in real-time PCR [ 92 , 93 , 94 ], fluorescent gel imaging [ 95 , 96 ], and flow cytometry [ 97 , 98 ].…”

Section: Small-molecule–aptamer Interaction and Characterizationmentioning

confidence: 99%

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

2022

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Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment. Therefore, it is crucial to develop low-cost, user-friendly, and portable biosensors capable of rapidly detecting these contaminants. Antibodies have traditionally been used as biorecognition elements. However, aptamers have recently been applied as biorecognition elements in aptamer-based biosensors, also known as aptasensors. The systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro technique used to generate aptamers that bind their targets with high affinity and specificity. Over the past decade, a modified SELEX method known as Capture-SELEX has been widely used to generate DNA or RNA aptamers that bind small molecules. In this review, we summarize the recent strategies used for Capture-SELEX, describe the methods commonly used for detecting and characterizing small-molecule–aptamer interactions, and discuss the development of aptamer-based biosensors for various applications. We also discuss the challenges of the Capture-SELEX platform and biosensor development and the possibilities for their future application.

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“…from corn rhizosphere as examples and are used as potential rhizosphere biomarkers (Table 5). [90–97] …”

Section: Aptamers For Pesticidesmentioning

confidence: 99%

“…from corn rhizosphere as examples and are used as potential rhizosphere biomarkers (Table 5). [90][91][92][93][94][95][96][97] In 2021, a DNA aptamer for L-serine, an exudate from the roots of wheat and canola, was selected by Mastronardi et al using multiple selection strategies. [98] The L-serine aptamers generated by SELEX had micromolar affinity.…”

Section: Fumonisinmentioning

confidence: 99%

Nucleic Acid Aptamers for Pesticides, Toxins, and Biomarkers in Agriculture

Shi²,

Tian

et al. 2022

ChemPlusChem

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Nucleic acid aptamers are short single-stranded DNA/RNA (ssDNA/RNA) oligonucleotides that can selectively bind to the targets. They are widely used in medicine, biosensing, and diagnostic assay. They have also been identified and extensively used for various targets in agriculture. In this review we summarize the progress of nucleic acid aptamers on pesticides (herbicides, insecticides, and fungicides), toxins, specific biomarkers of crops, and plant growth regulators in agricultural field in recent years. The basic process of aptamer selection, the already identified DNA/RNA aptamers and the aptasensors are discussed. We also discuss the future perspectives and the challenges for aptamer development in agriculture.

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Highly selective and sensitive detection of glutamate by an electrochemical aptasensor

Cited by 18 publications

References 82 publications

Toward the Next Generation of Neural Iontronic Interfaces

Toward the Next Generation of Neural Iontronic Interfaces

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

Nucleic Acid Aptamers for Pesticides, Toxins, and Biomarkers in Agriculture

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