Highly Repeated Sequences in Mammalian Genomes

Singer, Maxine

doi:10.1016/s0074-7696(08)61789-1

Cited by 631 publications

(319 citation statements)

References 211 publications

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“…The GC content was low (37.7%), and sequence homology searching using BLAST did not show the presence of any related DNA sequences. Taken together, these results suggest that this repetitive sequence is categorized as a novel satellite DNA sequence (Singer 1982) and is a good molecular cytogenetic marker for the W chromosome of O. hubbsi.…”

Section: Discussionmentioning

confidence: 60%

Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes

et al. 2011

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Among the medaka fishes of the genus Oryzias, most species have homomorphic sex chromosomes, while some species, such as Oryzias hubbsi and Oryzias javanicus, have heteromorphic ZW sex chromosomes. In this study, a novel family of repetitive sequence was molecularly cloned from O. hubbsi and characterized by chromosome in situ and filter hybridization, respectively. This repetitive element, which we designated as a BstNI family element, localized at heterochromatin regions on the W chromosome, as well as on two pairs of autosomes. Homologous sequences to this element were found only in O. javanicus, which is a sister species of O. hubbsi, suggesting that this repeated element originated in the common ancestor of these two species. However, the intensity of the hybridization signals was lower in O. javanicus than in O. hubbsi, and the chromosomal location of this element in O. javanicus was confined to heterochromatin regions on one pair of autosomes. Thus, we hypothesize that this repetitive element was extensively amplified in the O. hubbsi lineage, especially on its W chromosome, after the separation of the O. javanicus lineage. In addition, we also found the W chromosomal location of the 18S-28S ribosomal RNA genes in both O. hubbsi and O. javanicus. Our previous studies showed no linkage Chromosome Res (2012) 20:71-81

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Section: Discussionmentioning

confidence: 60%

Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes

et al. 2011

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“…Moreover, each variant class is tandemly clustered on the chromosome, physically separated from other variants. Such an arrangement as well as the existence of an undecamer within the basic units argues strongly for the evolution of the Ascaris satellite from a common ancestral sequence by the mechanism of sequence divergence followed by an amplification mechanism, as has been proposed already for other satellites (28)(29)(30)32). In a first step, the 120 bp segments evolved from the ancestral undecamer sequence.…”

Section: Structure and Organization Of The Satellite Dnamentioning

confidence: 58%

“…In Notophtalmus viridescens, transcription may initiate at promotor sites for histone genes which are embedded within variable stretches of satellite DNA sequences, and then proceed into the downstream satellite DNA sequences (35,36). Other functions that have been suggested for satellite DNA, not involving its transcription, are mainly based on its general association with heterochromatin (for review see [28][29][30][31][32] Nucleic Acids Research have to assume that the same function could be exerted in somatic tissues by the few remaining copies, which seems to be rather unlikely.…”

Section: Structure and Organization Of The Satellite Dnamentioning

confidence: 99%

Nucleotide sequence of satellite DNA contained in the eliminated genome ofAscaris lumbricoides

et al. 1982

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“…Long interspersed repeated DNA families [LINE (6) or Li families (7)] are one class of highly repeated DNA families that together account for a third or more of various mammalian genomes (6,8). Although the evolutionary origin and present day function of LINE DNA is not known, this class of sequences, like other highly repeated interspersed sequences, must have possessed at some time, or have been unusually susceptible to, one or more means of amplification and transposition.…”

Section: Introductionmentioning

confidence: 99%

Target sites for the transposition of rat long interspersed repeated DNA elements (LINEs) are not random

Furano¹,

Somerville²,

Tsichlis

et al. 1986

Nucl Acids Res

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The long interspersed repeated DNA family of rats (LINE or LIRn family) contains about 40,000 6.7-kilobase (kb) long members (1). LINE members may be currently mobile since their presence or absence causes allelic variation at three single copy loci (2, 3): insulin 1, Moloney leukemia virus integration 2 (Mlvi-2) (4), and immunoglobulin heavy chain (Igh). To characterize target sites for LINE insertion, we compared the DNA sequences of the unoccupied Mlvi-2 target site, its LINE-containing allele, and several other LINE-containing sites. Although not homologous overall, the target sites share three characteristics: First, depending on the site, they are from 68% to 86% (A+T) compared to 58% (A+T) for total rat DNA (5). Depending on the site, a 7-to 15-bp target site sequence becomes duplicated and flanks the inserted LINE member. The second is a version (O or 1 mismatch) of the hexanucleotide, TACTCA, which is also present in the LINE member, in a highly conserved region located just before the A-rich right end of the LINE member.The third is a stretch of alternating purine/pyrimidine (PQ). The A-rich right ends of different LINE members vary in length and composition, and the sequence of a particularly long one suggests that it contains the A-rich target site from a previous transposition.

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Highly Repeated Sequences in Mammalian Genomes

Cited by 631 publications

References 211 publications

Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes

Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes

Nucleotide sequence of satellite DNA contained in the eliminated genome ofAscaris lumbricoides

Target sites for the transposition of rat long interspersed repeated DNA elements (LINEs) are not random

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