Highly Regulated Expression of Subtilisin-like Proprotein Convertase PACE4 (SPC4) during Dentinogenesis

Akamatsu, Tetsuya; Matsuda, Yoshiko; Tsumura, Keiko; Tada, J; Parvin, Most. Nahid; Wei, Wei; Kanamori, Norio; Hosoi, Kazuo

doi:10.1006/bbrc.2000.2752

Cited by 16 publications

(19 citation statements)

References 29 publications

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“…PCSK6 (protein convertase subtilisin/kexin type 6) is an endoprotease that together with other protein convertases regulates the activity of a large variety of cellular proteins, e.g., growth factors, receptors, and enzymes (59). PCSK6 was described to be colocalized with several BMPs, especially with BMP4, and its expression was found to be highly regulated during dentinogenesis (2).…”

Section: Discussionmentioning

confidence: 99%

Molecular signatures and new candidates to target the pathogenesis of rheumatoid arthritis

Ungethuem

Haeupl

Witt

et al. 2010

Physiological Genomics

115

103

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Section: Discussionmentioning

confidence: 99%

Molecular signatures and new candidates to target the pathogenesis of rheumatoid arthritis

Ungethuem

Haeupl

Witt

et al. 2010

Physiological Genomics

115

103

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“…Isolation of total RNA from the developing rat SMG Total RNAs were isolated from the SMG of 16.5-, 17.5-, 18.5-, 19.5-, 20.5-and 21.5-day rat embryos and 0-, 1-, 2-, 5-, 6-, 7-, 11-, 14-, 15-, 17-, 19-, 21-and 25-day-old postnatal rats using TRI reagent (Sigma, St. Louis, Mo., USA) according to the manufacturer's protocol, as described previously [3].…”

Section: Methodsmentioning

confidence: 99%

“…Reverse transcriptase-polymerase chain reaction RT-PCR was performed by using a kit (SuperScript One-Step RT-PCR System, Invitrogen, Carlsbad, Calif., USA) according to the manufacturer's protocol as earlier described [3]. Total RNA (1 g) from a gland at each stage was transcribed and amplified in a 25-l reaction volume composed of 1reaction mix, 5 pmol each polynucleotide of a primer set and 0.5 l RT/Taq mix.…”

Section: Methodsmentioning

confidence: 99%

“…A DIG-labelled antisense cRNA probe for each AQP was generated by in vitro transcription as described previously [2,3]. Instead of using the antisense primer shown in Table 1, we amplified the individual rat AQP cDNAs by RT-PCR using the antisense primer tagged with a promoter sequence for T7 RNA polymerase.…”

Section: Synthesis Of Digoxigenin (Dig)-labelled Antisense Crna Probesmentioning

confidence: 99%

“…Northern blot analysis RNA samples were analysed by Northern blot as described previously [3]. Total RNA (10 g) was resolved by electrophoresis in a 1% agarose gel containing 6.7% formaldehyde and transferred onto a nylon membrane, Hybond-N+ (Amersham Pharmacia Biotech, England).…”

Section: Synthesis Of Digoxigenin (Dig)-labelled Antisense Crna Probesmentioning

confidence: 99%

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Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland

Akamatsu

Parvin

Murdiastuti

et al. 2003

Pflugers Arch - Eur J Physiol

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The expression and localization of aquaporins (AQP1-AQP5), members of the water channel family, in the developing rat submandibular gland were analysed using RT-PCR, Northern blotting and immunohistochemistry to explore their relation to the development of this salivary gland. RT-PCR analysis revealed unique expression patterns of each AQP. AQP1 was expressed constitutively during prenatal development, whereas the expression of AQP5 became more intense in the course of development from embryonic day 16.5 (E16) to E20. These expression patterns concurred with the results of Northern blot analysis. AQP3 and AQP4 mRNAs in the prenatal development were not detected in Northern blots, although they were detected by RT-PCR. During postnatal development, AQP5 and AQP1 mRNAs were expressed continuously, but no message for AQP3 or AQP4 was detected. AQP2 mRNA was not detected during either prenatal or postnatal development in this tissue. Immunohistochemical studies revealed that AQP5 was first localized at the apical membrane of proacinar cells at E18, and then became clearly distributed at the apical membrane of acinar cells in accordance with the differentiation and establishment of the mature acini. In addition, some vasculature also showed immunoreactivity for AQP5. AQP1 was immunolocalized in the blood vessels, including capillaries, of the gland throughout development. These observations suggest the existence of transcriptional regulation of rat AQP5, which is one of the most probable regulators of saliva production and secretion, during the establishment of the functional submandibular salivary gland.

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Temporospatially regulated expression of subtilisin‐like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland

Akamatsu

Purwanti

Karabasil

et al. 2006

Developmental Dynamics

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The temporospatial expression of PACE4, a member of the mammalian subtilisin-like proprotein convertase family involved in the activation of growth/differentiation factors, was investigated by in situ hybridization during the development of the rat submandibular gland (SMG). At the initiation stage (day 15.5 of gestation; E15), PACE4 was intensely expressed in the submandibular epithelium, but weakly expressed in the mesenchymal cells. At E16 when the branching morphogenesis becomes obvious, the expression of PACE4 in the mesenchyme was further decreased, although its level in the submandibular epithelium had not changed remarkably from that at E15. During the next stage of embryonic development (E17-E20), PACE4 was expressed in the cells derived from the submandibular epithelium, which include the proacinar, terminal tubular, and presumptive ductal cells. In the perinatal SMG, PACE4 was still expressed intensely in the terminal portion of the SMG containing the proacinar and terminal tubular cells, whereas its expression in the ductal cells was obviously decreased at the second postnatal day (P2) and at P6. Acinar cells expressing no PACE4 appeared, and their numbers increased following their development (P9-P20). At P30 when the PACE4 expression in the acinar cells was completely suppressed, its expression in the ductal cells became intense again. This temporospatially regulated expression of PACE4 suggests its apparent association with the proliferation, differentiation, and establishment of functional acinar and ductal cells of the SMG.

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Highly Regulated Expression of Subtilisin-like Proprotein Convertase PACE4 (SPC4) during Dentinogenesis

Cited by 16 publications

References 29 publications

Molecular signatures and new candidates to target the pathogenesis of rheumatoid arthritis

Molecular signatures and new candidates to target the pathogenesis of rheumatoid arthritis

Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland

Temporospatially regulated expression of subtilisin‐like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland

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