Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

Guex, Anne Géraldine; Puetzer, Jennifer L.; Armgarth, Astrid; Littmann, Elena; Stavrinidou, Eleni; Giannelis, Emmanuel P.; Malliaras, George G.; Stevens, Molly M.

doi:10.1016/j.actbio.2017.08.045

Cited by 206 publications

(208 citation statements)

References 57 publications

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“…The normalized conductivity of the PEDOT‐functionalized aerogels was calculated to be 146 ± 8 S m −1 . This value compares well with the one reported in recently published studies regarding aerogels solely made from freeze‐dried PEDOT:PSS dispersions . This result is remarkable since the cellulose and alginate represent a large weight fraction of the material (37% at a loading of 1.7 g g −1 ), and can be considered as electrical insulators.…”

Section: Pedot:tos‐functionalized Aerogelssupporting

confidence: 89%

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Françon

Wang

Marais

et al. 2020

Adv Funct Materials

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This study presents a novel, green, and efficient way of preparing crosslinked aerogels from cellulose nanofibers (CNFs) and alginate using non‐covalent chemistry. This new process can ultimately facilitate the fast, continuous, and large‐scale production of porous, light‐weight materials as it does not require freeze‐drying, supercritical CO2 drying, or any environmentally harmful crosslinking chemistries. The reported preparation procedure relies solely on the successive freezing, solvent‐exchange, and ambient drying of composite CNF‐alginate gels. The presented findings suggest that a highly‐porous structure can be preserved throughout the process by simply controlling the ionic strength of the gel. Aerogels with tunable densities (23–38 kg m−3) and compressive moduli (97–275 kPa) can be prepared by using different CNF concentrations. These low‐density networks have a unique combination of formability (using molding or 3D‐printing) and wet‐stability (when ion exchanged to calcium ions). To demonstrate their use in advanced wet applications, the printed aerogels are functionalized with very high loadings of conducting poly(3,4‐ethylenedioxythiophene):tosylate (PEDOT:TOS) polymer by using a novel in situ polymerization approach. In‐depth material characterization reveals that these aerogels have the potential to be used in not only energy storage applications (specific capacitance of 78 F g−1), but also as mechanical‐strain and humidity sensors.

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Section: Pedot:tos‐functionalized Aerogelssupporting

confidence: 89%

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Françon

Wang

Marais

et al. 2020

Adv Funct Materials

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“…[5][6][7] The conducting properties of organic biopolymers originate from the presence of conjugated double bonds throughout the polymer chain, with the conductivity depending on the bandgap between the conduction and valence bands. [9] Therefore, in this study, we tested PEDOT:PSS as a polymeric bioelectrode for manipulating cellular behavior under ES. Because of their low toxicity, PEDOT:PSS blends are also potential bioelectrode materials for use within organic bioelectronics.…”

mentioning

confidence: 99%

Poly(3,4‐ethylenedioxythiophene) Polymer Composite Bioelectrodes with Designed Chemical and Topographical Cues to Manipulate the Behavior of PC12 Neuronal Cells

Tsai

She

et al. 2019

Adv Materials Inter

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Controlled extracellular chemical and topographical cues can generate physicochemical changes that influence the proliferation and differentiation of neural cells; external electrical stimulation (ES) via conductive bioelectrodes can promote neural differentiation by increasing neurite outgrowth. Rat pheochromocytoma (PC12) cells are used as a neuron‐like model cells to explore the possibility of using a well‐designed poly(ethylene oxide) (PEO)/poly(3,4‐ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) blend solution to fabricate functional bioelectrodes presenting biological fouling/antifouling surfaces, thereby regulating the cell adhesion, proliferation, and differentiation properties. In this study, it is found that a flat PEO/PEDOT:PSS composite film fabricates through spin‐coating, operates through a contact repulsion mechanism that limits cell attachment and proliferation; in contrast, the aligned and random PEO/PEDOT:PSS nanofibers fabricates through electrospinning promoted neuron adhesion efficiently and allows manipulation of the cell morphology. Furthermore, ES of PC12 cells is performed to investigate the influence of the conductive random and aligned PEO/PEDOT:PSS composite nanofiber mats on the enhancement of neurite outgrowth, as well as the relative gene expression of Nestin, Tuj1, and MAP2. Therefore, combining this unique PEDOT:PSS blend solution with various fabrication processes appears to be a facile approach toward bioelectronic interface coatings displaying tunable surface properties for manipulating the cellular behavior of neurons during ES.

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“…Within bone tissues, generation of electrical signals is a quite common phenomenon, which can be caused by events such as the shear‐force of collagen and the deformation of fluid‐filled channels, and in return, these electrical signals may be vital in deciding osteogenesis . Inspired by this special bioelectricity of bone tissue, electrical stimulation (ES) in combination with conductive scaffolding materials is available as an adjunctive therapy used to enhance bone healing …”

Section: Introductionmentioning

confidence: 99%

“…Researches concerning the effects of ES on cell biological behaviors are commonly carried out on conductive substrates, which help in translating ES to those cells cultured on the substrates effectively and mimicking the electrophysiological environment in native tissues . From diverse reports, it has been confirmed that conductive substrates are of excellent osteocompatibility, and display significant promotion on osteogenesis . Biocompatible polymers with conductivity like polypyrrole (PPY), polyaniline (PANI), and poly(3,4‐ethylenedioxythiophene) (PEDOT) are widely applied in tissue regeneration studies, especially for bone, nerve, and myocardial tissue regeneration .…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Inspired by this special bioelectricity of bone tissue, electrical stimulation (ES) in combination with conductive scaffolding materials is available as an adjunctive therapy used to enhance bone healing. [6][7][8] Researches concerning the effects of ES on cell biological behaviors are commonly carried out on conductive substrates, which help in translating ES to those cells cultured on the substrates effectively and mimicking the electrophysiological environment in native tissues. 9,10 From diverse reports, it has been confirmed that conductive substrates are of excellent osteocompatibility, and display significant promotion on osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Roles of electrical stimulation in promoting osteogenic differentiation of BMSCs on conductive fibers

Wei

Huang

Wei

et al. 2019

J Biomedical Materials Res

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The strategy of using conductive materials in regenerating bone defects is attractive, benefiting from the bioelectricity feature of natural bone tissues. Thereby, POP conductive fibers were fabricated by coating polypyrrole (PPY) onto electrospun poly(l‐lactide) (PLLA) fibers, and their potentials in promoting osteogenic differentiation of bone mesenchymal stromal cells (BMSCs) were investigated. Different from the smooth‐surfaced PLLA fibers, POP fibers were rough‐surfaced and favorable for protein adsorption and mineralization nucleation. When electrical stimulation (ES) was applied, the surface charges on the conductive POP fibers further promoted the protein adsorption and the mineral deposition, while the non‐conductive PLLA fibers displayed no such promotion. When BMSCs were cultured on these fibers, strong cell viability was detected, indicating their good biocompatibility and cell affinity. In osteogenic differentiation studies, BMSCs demonstrated the strongest ability in differentiating toward osteoblasts when they were cultured on the POP fibers under ES, followed by the case without ES. In comparison with the conductive POP fibers, the non‐conductive PLLA fibers displayed significantly weaker ability in inducing the osteogenic differentiation of BMSCs with ES being applied or not. Alongside the differentiation, both the calcium deposition on BMSC/material complexes and the intracellular Ca2+ concentration were identified the most abundant when BMSCs grew on the POP fibers under ES. These findings suggested that the surface charges of conductive fibers played roles in regulating protein adsorption, ion migration and nucleation, particularly under ES, which contributed much to the increased intracellular Ca2+ ions, and thus accelerated the osteogenic differentiation of the seeded cells. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

Abstract: Graphical abstract

Cited by 206 publications

References 57 publications

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Poly(3,4‐ethylenedioxythiophene) Polymer Composite Bioelectrodes with Designed Chemical and Topographical Cues to Manipulate the Behavior of PC12 Neuronal Cells

Roles of electrical stimulation in promoting osteogenic differentiation of BMSCs on conductive fibers

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