Highly Porous, Molecularly Imprinted Core–Shell Type Boronate Affinity Sorbent with a Large Surface Area for Enrichment and Detection of Sialic Acid Isomers

Kip, Çiğdem; Demir, Mustafa Cihan; Yıldırım, Duygu; Hamaloğlu, Kadriye Özlem; Çelikbıçak, Ömür; Tuncel, Alï

doi:10.1007/s10904-021-01890-w

Cited by 2 publications

(1 citation statement)

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“…Many methods have been developed for the detection of sialic acids on the cell membrane surface (Table S2 ). For example, inductively coupled plasma mass spectrometry has been utilized to detect sialic acids on the surface of different cell membrane [ 45 ]. Quartz crystal microbalance method can sensitively detect sialic acid on the surface of red blood cells by using gold nanoparticles for signal amplification [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

et al. 2022

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Evaluating tumor development is of great importance for clinic treatment and therapy. It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell. So, in this work, cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids, i.e., N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac). Specifically, PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface, followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin. Subsequently, the activated Cas12a indiscriminately cleaves DNA, resulting in the release of PbS and CdS quantum dots, both of which can be simultaneously detected by anodic stripping voltammetry. Consequently, Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL, respectively. Therefore, a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface, which can be further used as a tool to identify bladder cancer cells at different development stages. Our method to evaluate tumor development is simple and easy to be operated, so it can be potentially applied for the detection of tumor occurrence and development in the future.

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Section: Discussionmentioning

confidence: 99%