Highly photostable wide-dynamic-range pH sensitive semiconducting polymer dots enabled by dendronizing the near-IR emitters

Chen, L.; Li, Wu; Yu, Jian; Kuo, Chun-Ting; Jian, Tang; Wu, I‐Chin; Rong, Yu; Chiu, Daniel T.

doi:10.1039/c7sc03448b

Cited by 49 publications

(33 citation statements)

References 78 publications

(76 reference statements)

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“…Compared with organic dyes and quantum dots, Pdots show higher absorptivity and brightness and better photostability and biocompatibility . The oxygen‐sensitive polymer was synthesized by a Suzuki coupling reaction .…”

Section: Methodsmentioning

confidence: 99%

Compact Conjugated Polymer Dots with Covalently Incorporated Metalloporphyrins for Hypoxia Bioimaging

Fang

Liu

et al. 2018

ChemBioChem

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Hypoxia is closely related to multiple diseases, especially in tumors, which increases the aggressiveness and drug resistance of cancer cells. Precise hypoxia imaging is of great significance for cancer diagnosis and the evaluation of therapeutic effects. A kind of hydrophobic polymer (i.e., PFPtTFPP) as an imaging probe for hypoxia with fluorene as an energy donor and an oxygen‐sensitive PtII porphyrin as an energy acceptor was developed. Compact polymer dots (Pdots) with a small size were prepared by nanoprecipitation. The PFPtTFPP Pdots showed excellent hypoxia sensing in solution with high sensitivity and full reversibility. The emission intensity, quantum yields, lifetime, and single‐particle brightness significantly increased under hypoxia conditions. Remarkably, hypoxia imaging in vitro and in vivo was realized, and a clear increase in brightness was observed under hypoxia conditions and in the tumor area. Excellent hypoxia imaging ability is beneficial to potential applications in cancer diagnosis.

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Section: Methodsmentioning

confidence: 99%

Compact Conjugated Polymer Dots with Covalently Incorporated Metalloporphyrins for Hypoxia Bioimaging

Fang

Liu

et al. 2018

ChemBioChem

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“…[61][62][63][64] Our group designed a type of Pb 2+ -sensitive Pdots in which the polymer/dye matrices were encapsulated by polydiacetylenes (PDAs) with 15-crown-3 as the functional groups. [36] Copyright 2017, the Royal Society of Chemistry Pb 2+ , the strain of the PDA structure was perturbed, resulting in the chromatic transition from blue to red. [60] Upon the chelation of [60] Copyright 2015, American Chemical Society.…”

Section: Ion Detectionmentioning

confidence: 99%

“…[36] They found that the incorporation of large steric groups on the polymer sidechains could eliminate the ACQ effect and reduce photobleaching in the aggregated state, leading to higher NIR fluorescence and exceptional pH sensing performance. Chiu's lab designed a series of squaraine-based Pdots conjugated with pH-responsive NIR fluorophores.…”

Section: Ph Sensingmentioning

confidence: 99%

Semiconducting polymer dots as near‐infrared fluorescent probes for bioimaging and sensing

Tsai

Chan

2018

J Chinese Chemical Soc

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In recent years, semiconducting polymer dots (Pdots) have emerged as a new type of ultrabright fluorescent probes, which have been proved to be very useful for biomedical imaging. Pdots possess several exceptional advantages including high fluorescence brightness, fast radiative rate, excellent photostability, and negligible cytotoxicity. Among these new types of Pdots, the near‐infrared (NIR) fluorescent Pdots appear to be the most urgent and important owing to their promising deep‐tissue imaging in the clinic. This mini‐review highlights the recent progress in the design of NIR‐emitting Pdots and their biomedical applications both in vitro and in vivo.

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“…Semiconducting polymer nanoparticles (SPNs) have recently shown appealing performances as imaging probes and therapeutic agents owing to their high fluorescence brightness, nonblinking behavior, excellent photostability, and good biocompatibility. Owing to the amplified energy transfer ability of SPNs, some fluorescence resonance energy transfer (FRET)‐based SPNs probe for ratiometric detection of metal ion, biomolecule, as well as intracellular pH value and temperature have been reported. However, most of these systems are based on energy transfer from SPNs to small molecules, which are either susceptible to photobleaching or easy to leach from SPNs.…”

Section: Introductionmentioning

confidence: 99%

Highly Stable Core–Shell Structured Semiconducting Polymer Nanoparticles for FRET‐Based Intracellular pH Imaging

Bao

Yang

et al. 2019

Adv Healthcare Materials

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degradation. [4][5][6][7] Intracellular pH is tightly regulated within a narrow range (6.8-7.2) in the cytosol through the ability of membrane proton pumps and transporters. An abnormal pH level has also been demonstrated to trigger many diseases including Alzheimer's disease, cancer, and stroke. [8][9][10][11] Hence, designing stable and reliable probes for the detection of intracellular pH changes is necessary for studying intracellular events and would be critical to understand the biological role of pHi.Semiconducting polymer nanoparticles (SPNs) have recently shown appealing performances as imaging probes [12][13][14] and therapeutic agents [15,16] owing to their high fluorescence brightness, nonblinking behavior, excellent photostability, and good biocompatibility. Owing to the amplified energy transfer ability of SPNs, some fluorescence resonance energy transfer (FRET)based SPNs probe for ratiometric detection of metal ion, [17][18][19] biomolecule, [20][21][22] as well as intracellular pH value [23,24] and temperature [25] have been reported. However, most of these systems are based on energy transfer from SPNs to small molecules, which are either susceptible to photobleaching or easy to leach from SPNs. Furthermore, direct coprecipitation of reference dye and signal indicator may pose an interference of target-responsive group on reference dye emission. Therefore, the exploration of SPNs-based ratiometric imaging probes, which combines structural as well as optical stability and consistent ratiometric emission intensities are still highly desired but remain to be revealed.Here, we propose a new approach for the preparation of ratiometric probes (PFO/PFV-DA NPs) with a previously unreported core-shell structure for intracellular pH sensing. Semiconducting polymer PFO and PFV were selected as central donor and shell acceptor respectively because both PFO and PFV exhibit signal amplification property, excellent photostability, and biocompatibility. In additional, core-shell structured ratiometric nanoprobe with all semiconducting polymer composition will reduces leaching of materials, which is ideal for intracellular sensing and in vivo imaging. Importantly, Our PFO/PFV-DA NPs have completely different structure of PFO/PFV core-shell compared to previous reports, where PFO Highly stable semiconducting polymer nanoparticles (NPs) (poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO)/ poly(fluorene-2,7-ylenevinylene-cophenylene) (PFV)-dopamine (DA) NPs) with previously unreported core-shell structure are developed for ratiometric sensing of intracellular pH values. PFO/ PFV-DA NPs comprise central polyfluorene (PFO) as donor and PFV as acceptor, in which the donor and acceptor are spatially separated into the central core and nanoparticle shell. Specifically, thick PFV shells can not only significantly minimize the quenching interference of dopamine on the emission of standard reference (PFO), but are also able to maximize its accessibility to pH-sensitive dopamine and lead to sensitive response to pH changes. The resul...

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Highly photostable wide-dynamic-range pH sensitive semiconducting polymer dots enabled by dendronizing the near-IR emitters

Abstract: A highly photostable, ultra-bright, biocompatible and NIR emissive semiconducting polymer dot (Pdot) based pH sensor was achieved by dendronizing the squaraine probe molecule.

Cited by 49 publications

References 78 publications

Compact Conjugated Polymer Dots with Covalently Incorporated Metalloporphyrins for Hypoxia Bioimaging

Compact Conjugated Polymer Dots with Covalently Incorporated Metalloporphyrins for Hypoxia Bioimaging

Semiconducting polymer dots as near‐infrared fluorescent probes for bioimaging and sensing

Highly Stable Core–Shell Structured Semiconducting Polymer Nanoparticles for FRET‐Based Intracellular pH Imaging

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